Passive sampling to scale wastewater surveillance of infectious disease: Lessons learned from COVID-19

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 835, P. 155347 - 155347

Published: April 21, 2022

Much of what is known and theorized concerning passive sampling techniques has been developed considering chemical analytes. Yet, historically, biological analytes, such as Salmonella typhi, have collected from wastewater via with Moore swabs. In response to the COVID-19 pandemic, re-emerging a promising technique monitor SARS-CoV-2 RNA in wastewater. Method comparisons disease surveillance using composite, grab, for detection found variety materials routinely produced qualitative results superior grab samples useful sub-sewershed COVID-19. Among individual studies, concentrations derived samplers demonstrated heterogeneous correlation paired composite ranging weak (R2 = 0.27, 0.31) moderate 0.59) strong 0.76). sampler materials, electronegative membranes shown great promise linear uptake observed exposure durations 24 48 h several cases positivity on par samples. Continuing development methods infectious diseases diverse forms fecal waste should focus optimizing efficient recovery kit-free extraction, resource-efficient testing capable rapidly producing or quantitative data. With refinements could prove be fundamental tool scaling disease, especially among 1.8 billion persons living low-resource settings served by non-traditional collection infrastructure.

Language: Английский

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Tracing COVID-19 Trails in Wastewater: A Systematic Review of SARS-CoV-2 Surveillance with Viral Variants

Water, Journal Year: 2023, Volume and Issue: 15(6), P. 1018 - 1018

Published: March 7, 2023

The emergence of new variants SARS-CoV-2 associated with varying infectivity, pathogenicity, diagnosis, and effectiveness against treatments challenged the overall management COVID-19 pandemic. Wastewater surveillance (WWS), i.e., monitoring infections in communities through detecting viruses wastewater, was applied to track spread globally. However, there is a lack comprehensive understanding use WWS for variants. Here we systematically reviewed published articles reporting different wastewater by following PRISMA guidelines provided current state art this study area. A total 80 studies were found that reported until November 2022. Most these (66 out 80, 82.5%) conducted Europe North America, resource-rich countries. There high variation sampling strategy around world, composite (50/66 studies, 76%) as primary method In contrast, grab more common (8/14 57%) resource-limited Among detection methods, reverse transcriptase polymerase chain reaction (RT-PCR)-based sequencing quantitative RT-PCR commonly used wastewater. variants, B1.1.7 (Alpha) variant appeared earlier pandemic most (48/80 studies), followed B.1.617.2 (Delta), B.1.351 (Beta), P.1 (Gamma), others All same pattern clinical within timeline, demonstrating tracked all timely way when emerged. Thus, may be utilized identify presence or absence follow development transmission existing emerging Routine powerful infectious disease tool implemented

Language: Английский

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Structured Ethical Review for Wastewater-Based Testing in Support of Public Health

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(35), P. 12969 - 12980

Published: Aug. 23, 2023

Wastewater-based testing (WBT) for SARS-CoV-2 has rapidly expanded over the past three years due to its ability provide a comprehensive measurement of disease prevalence independent clinical testing. The development and simultaneous application WBT measured biomarkers research activities pursuit public health goals, both areas with well-established ethical frameworks. Currently, practitioners do not employ standardized review process, introducing potential adverse outcomes professionals community members. To address this deficiency, an interdisciplinary workshop developed framework structured WBT. employed consensus approach create as set 11 questions derived from primarily guidance. This study retrospectively applied these monitoring programs covering emergent phase pandemic (3/2020-2/2022 (

Language: Английский

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Performance evaluation of virus concentration methods for implementing SARS-CoV-2 wastewater based epidemiology emphasizing quick data turnaround

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 801, P. 149656 - 149656

Published: Aug. 14, 2021

Wastewater based epidemiology (WBE) has drawn significant attention as an early warning tool to detect and predict the trajectory of COVID-19 cases in a community, conjunction with public health data. This means monitoring for outbreaks been used at municipal wastewater treatment centers analyze trends entire communities, well by universities other community living environments monitor spread buildings. Sample concentration is crucial, especially when viral abundance raw below threshold detection RT-qPCR analysis. We evaluated performance rapid ultrafiltration-based virus method using InnovaPrep Concentrating Pipette (CP) Select compared this established electronegative membrane filtration (EMF) method. sensitivity SARS-CoV-2 quantification, surrogate recovery rate, sample processing time. Results suggest that CP concentrator more efficient concentrating from EMF About 25% samples tested negative concentrated produced positive signal protocol. Increased control confirms observation. optimized protocol adding AVL lysis buffer sonication, increase virus. Sonication increased Bovine Coronavirus (BCoV) 19%, which seems compensate loss during centrifugation. Filtration time decreases approximately 30% protocol, making optimal choice building surveillance applications where quick turnaround necessary.

Language: Английский

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Assessment of two types of passive sampler for the efficient recovery of SARS-CoV-2 and other viruses from wastewater

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 838, P. 156580 - 156580

Published: June 9, 2022

Language: Английский

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Research needs for optimising wastewater-based epidemiology monitoring for public health protection

Journal of Water and Health, Journal Year: 2022, Volume and Issue: 20(9), P. 1284 - 1313

Published: Aug. 12, 2022

Abstract Wastewater-based epidemiology (WBE) is an unobtrusive method used to observe patterns in illicit drug use, poliovirus, and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). The pandemic need for surveillance measures have led the rapid acceleration of WBE research development globally. With infrastructure available monitor SARS-CoV-2 from wastewater 58 countries globally, there potential expand targets applications public health protection, such as other viral pathogens, antimicrobial resistance (AMR), pharmaceutical consumption, or exposure chemical pollutants. Some been explored academic but are not inform decision-making. We reflect on current knowledge these identify barriers opportunities expanding beyond SARS-CoV-2. This paper critically reviews identifies important gaps be a useful tool health. It considers possible uses pathogenic viruses, AMR, chemicals. summarises evidence following: (1) presence markers stool urine; (2) environmental factors influencing persistence wastewater; (3) methods sample collection storage; (4) prospective detection quantification; (5) reducing uncertainties; (6) further considerations use.

Language: Английский

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Comparison of Auto Sampling and Passive Sampling Methods for SARS-CoV-2 Detection in Wastewater

Pathogens, Journal Year: 2022, Volume and Issue: 11(3), P. 359 - 359

Published: March 16, 2022

Wastewater-based surveillance is emerging as an important tool for the COVID-19 pandemic trending. Current methods of wastewater collection, such grab and auto-composite sampling, have drawbacks that impede effective surveillance, especially from small catchments with limited accessibility. Passive samplers, which are more cost-effective require fewer resources to process, promising candidates monitoring SARS-CoV-2. Here, we compared traditional auto sampling passive SARS-CoV-2 detection in wastewater. A torpedo-style 3D-printed sampler device containing both cotton swabs electronegative filter membranes was used. Between April June 2021, fifteen samplers were placed at a local hospital's outflow alongside autosampler. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) used detect samples after processing RNA extraction. The swab membrane showed similar rates cycle threshold (Ct) values N1 N2 gene targets. method performed well grab/auto no significant differences between Ct values. There discrepant results on two days negative positive samples, might be related longer duration study. Overall, rapid, reliable, cost-effective, could alternative

Language: Английский

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Adsorption of SARS-CoV-2 onto granular activated carbon (GAC) in wastewater: Implications for improvements in passive sampling

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 847, P. 157548 - 157548

Published: July 23, 2022

Based on recent studies, passive sampling is a promising method for detecting SARS-CoV-2 in wastewater surveillance (WWS) applications. Passive has many advantages over conventional approaches. However, the potential benefits of are also coupled with apparent limitations. We established technique severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using electronegative filters. Though, it was evident that adsorption capacity filters constrained their use. This work intends to demonstrate an optimized granular activated carbon (GAC). Through bench-scale batch-adsorption studies and sewershed deployments, we characteristics two human feacal viruses (PMMoV CrAssphage) onto GAC. A pseudo-second-order model best-described kinetics either deionized (DI) water SARS-CoV-2, CrAssphage, PMMoV wastewater. In both laboratory experiments in-situ maximum amount adsorbed by GAC occurred at ~60 h wastewater, CrAssphage h. contrast, reached DI seeded after ~35 The equilibrium assay modeled quantity (q

Language: Английский

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Simple methods for early warnings of COVID-19 surges: Lessons learned from 21 months of wastewater and clinical data collection in Detroit, Michigan, United States

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 864, P. 161152 - 161152

Published: Dec. 23, 2022

Language: Английский

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Surveillance of SARS-CoV-2 in sewage from buildings housing residents with different vulnerability levels

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 872, P. 162116 - 162116

Published: Feb. 10, 2023

During the last two years, various restrictions have been set up to limit transmission of Coronavirus Disease (COVID-19). While these rules apply at a large scale (e.g., country-wide level) human-to-human virus that causes COVID-19, Severe Acute Respiratory Syndrome 2 (SARS-CoV-2), occurs small scale. Different preventive policies and testing protocols were implemented in buildings where COVID-19 poses threat elderly residences) or constitutes disruptive force schools). In this study, we sampled sewage from different (a school, university campus, residence, an residence) host residents levels vulnerability. Our main goal was assess agreement between SARS-CoV-2 concentration wastewater applied buildings. All using passive samplers while 24-h composite samples also collected residence. Results showed performed comparably well being cost-effective keep track prevalence. comparison sampling (passive vs. active) combined with strict clinical allowed us compare sensitivities methods. Active more sensitive than sampling, as former able detect prevalence 0.4 %, compared 2.2 % for sampling. The number COVID-19-positive individuals tracked clinically all monitored More frequent detection observed residential non-residential samplers. buildings, surveillance can be used complement regimes, remained positive even when no reported. Passive is useful building managers adapt their mitigation policies.

Language: Английский

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