Recent advances in keratinase production via protein engineering, breeding, and fermentation

Advanced Agrochem, Journal Year: 2024, Volume and Issue: 3(3), P. 188 - 196

Published: Sept. 1, 2024

The gene editing and synthetic biological tools have led to the implementation of diverse metabolic engineering approaches enhance production specific enzymes. Microbial keratinases can convert keratin wastes into valuable compounds for mankind. Since market cannot be satisfied by from wild hosts, it is obligatory develop hosts with high keratinase yields. intention this review evaluate microbial advancement through protein engineering, breeding techniques, fermentation optimization. main aim improve heat resistance ability catalytic activity employing mutagenesis methods. Moreover, modifying expression elements host are also two unique ways augment yield. Intending accelerate modified keratinase, attempts highlight optimization elements, such as promoter UTR, signal peptide, codon including strengthening precursor supply, membrane surface secretion pathways were explained here. Furthermore, essential optimize medium composition condition This addressed present advancements, difficulties, tendencies in field production, along its potential.

Language: Английский

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Keratin–PNIPAM Hybrid Microgels: Preparation, Morphology and Swelling Properties

Gels, Journal Year: 2024, Volume and Issue: 10(6), P. 411 - 411

Published: June 20, 2024

Combinations of synthetic polymers, such as poly(N-isopropylacrylamide) (PNIPAM), with natural biomolecules, keratin, show potential in the field biomedicine, since these hybrids merge thermoresponsive properties PNIPAM bioactive characteristics keratin. This synergy aims to produce that can respond environmental stimuli while maintaining biocompatibility and functionality, making them suitable for various medical biotechnological uses. In this study, we exploit keratin derived from wool waste textile industry, extracted via sulfitolysis, synthesize microgel. Utilizing two distinct methods-polymerization NIPAM (HYB-P) mixing preformed microgels (HYB-M)-resulted 20% 25% content, respectively. Dynamic light scattering (DLS) transmission electron microscopic (TEM) analyses indicated formation colloidal systems particle sizes around 110 nm HYB-P 518 HYB-M. The presence both systems, 25%, respectively, was confirmed by spectroscopic (FTIR NMR) elemental analyses. Distinct structural differences were observed between HYB-M, suggesting a graft copolymer configuration former hybrid complexation latter one. Furthermore, demonstrated temperature responsiveness akin pH responsiveness, underscoring their diverse biomedical applications.

Language: Английский

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Adsorption of Cadmium from Wastewater with Activated Carbons Derived from Pig Fur Biowaste: A Comparative Study of In-Situ and Ex-Situ Activation Routes

Heliyon, Journal Year: 2024, Volume and Issue: 10(18), P. e37768 - e37768

Published: Sept. 1, 2024

The environmental challenges associated with cadmium contamination in wastewater have necessitated the development of high-performing activated carbons (ACs) for effective treatment. Adsorption capacity depends on both surface area and adsorption-active functional groups developed adsorbent's during activation. Proper manipulation key process variables using appropriate activation route produces highly efficient economically viable ACs. This research investigates viability pig fur biowaste as a novel precursor two distinct methods-in-situ ex-situ. Using central composite design (CCD) Response Surface Methodology (RSM), study systematically examines effects impregnation ratio, carbonization temperature, time adsorption capacities resulting optimal conditions in-situ were found to be 691 °C, 175.11 min, an ratio 1.784 g/g, 91.57 %. For ex-situ activation, 468.8 80.81 2.915 which achieved higher 91.21 Both types maintained high efficiency after five regeneration cycles, indicating they are suitable long-term applications requiring repeated regeneration. Although methods produced ACs comparable removal efficiency, proved more due its lower temperature shorter processing time. demonstrates potential sustainable underutilized resource AC production highlights cost-effective approach producing high-performance adsorbents.

Language: Английский

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The degradation of chicken feathers by Ochrobactrum intermedium results in antioxidant and metal chelating hydrolysates and proteolytic enzymes for staphylococcal biofilm dispersion

3 Biotech, Journal Year: 2023, Volume and Issue: 13(6)

Published: May 20, 2023

Language: Английский

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Structure prediction, docking studies and molecular cloning of novel Pichia kudriavzevii YK46 metalloprotease (MetPr) for improvement of feather waste biodegradation

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Nov. 15, 2023

This study addresses the environmental risks associated with accumulation of keratin waste from poultry, which is resistant to conventional protein degradation methods. To tackle this issue, microbial keratinases have emerged as promising tools for transforming resilient materials into valuable products. We focus on Metalloprotease (MetPr) gene isolated novel Pichia kudriavzevii YK46, sequenced, and deposited in NCBI GenBank database accession number OQ511281. The MetPr encodes a consisting 557 amino acids demonstrates keratinase activity 164.04 U/ml. 3D structure was validated using Ramachandran's plot, revealing that 93% 97.26% residues were situated within most favoured region proteins template strain 129 respectively. Computational analyses employed determine binding affinities between deduced beta keratin. Molecular docking studies elucidated optimal metalloprotease beta-keratin, yielding values - 260.75 kcal/mol 257.02 strains Subsequent molecular cloning expression E. coli DH5α led significantly higher 281 ± 12.34 These findings provide insights potential its encoded biotransformation, implications addressing concerns related keratinous accumulation.

Language: Английский

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