Improved Passive Sampling Methods for Wastewater to Enable More Sensitive Detection of Sars-Cov-2 and its Variants DOI
Md Alamin,

Pelumi Oladipo,

James Hartrick

et al.

Published: Jan. 1, 2024

Early detection of SARS-CoV-2 in wastewater is key to mitigating the spread new outbreaks COVID-19. Wastewater-based epidemiology can surveil communities while preserving individual anonymity, especially as clinical testing for COVID-19 has decreased. However, grab samples may miss marker presence, and auto-sampling throughout a day technically challenging costly. Tampon swabs passively collect markers over hours, but recovery captured challenge. Our goal was develop more efficient tampon elution methods virus variant analysis. Counts N1 N2 were compared eluted from tampons that had been immersed 3 sewersheds 4 - 6 hr during June December 2023. Tampons either by "SwabM" adding 20 mL extraction buffer (0.5X TE +0.075% Tween-20), or "SwabM1" directly squeezed 50 syringe, "SwabM2" syringe after 2 0.5 X + 0.075% Tween-20. A subset extracted sequenced compare variants versus samples. method SwabM averaged two- three-fold higher counts than paired (p<0.0001). Eluting SwabM2 gave five-fold signals & double (both, p<0.0001) trended SwabM1 (3 5-fold grab, p<0.001). Method also quality sequences likely have enough nucleic acids sequencing. Variant analysis Fall 2023 transition XBB JN "H" lineages. Collecting with – hours improves SARS-CoV-2, potentially enabling earlier identification variants. The yields counts, detections near Limit Detection, sequences.

Language: Английский

Wastewater surveillance for viral pathogens: A tool for public health DOI Creative Commons
Matheus Carmo dos Santos,

Ana Clara Cerqueira Silva,

Carine dos Reis Teixeira

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(13), P. e33873 - e33873

Published: June 29, 2024

A focus on water quality has intensified globally, considering its critical role in sustaining life and ecosystems. Wastewater, reflecting societal development, profoundly impacts public health. Wastewater-based epidemiology (WBE) emerged as a surveillance tool for detecting outbreaks early, monitoring infectious disease trends, providing real-time insights, particularly vulnerable communities. WBE aids tracking pathogens, including viruses, sewage, offering comprehensive understanding of community health lifestyle habits. With the rise global COVID-19 cases, gained prominence, aiding SARS-CoV-2 levels worldwide. Despite advancements treatment, poorly treated wastewater discharge remains threat, amplifying spread water-, sanitation-, hygiene (WaSH)-related diseases. WBE, serving complementary surveillance, is pivotal community-level viral infections. However, there untapped potential to expand surveillance. This review emphasizes importance link between health, highlighting need further integration into management.

Language: Английский

Citations

9
To sample or not to sample: A governance-focused decision tree for wastewater service providers considering participation in wastewater-based epidemiology (WBE) in support of public health programs DOI
Rasha Maal‐Bared, Kari Fitzmorris Brisolara, Mark A. Knight

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 905, P. 167128 - 167128

Published: Sept. 16, 2023

Language: Английский

Citations

4
Improved passive sampling methods for wastewater to enable more sensitive detection of SARS-CoV-2 and its variants DOI
Md Alamin,

Pelumi Oladipo,

James Hartrick

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 950, P. 175044 - 175044

Published: July 27, 2024

Language: Английский

Citations

1
Title: Qualitative Assessment of a Novel Results-Based Partnership between National Wastewater Surveillance Centers of Excellence and Utility Companies, Houston (Texas), Colorado, Wisconsin, and California, 2023. DOI Creative Commons
Hannah Turner, Libby Horter, Michael Welton

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Abstract Background The U.S. Centers for Disease Control and Prevention (CDC) initiated the National Wastewater Surveillance System (NWSS) in September 2020. Four of Excellence (COEs) were established between 2021–2023 Houston (Texas), Colorado, Wisconsin, California to guide wastewater surveillance efforts public health. Our objective was increase understanding factors that facilitated implementation from perspectives experiences shared by health department COEs utility partners. Methods We used a purposive sampling strategy select eight key respondents four NWSS respective conducted in-depth interviews related identified common points interview transcription files. Insights on WWS partners distilled responses into lessons learned. Results Three primary themes emerged after we analyzed responses: perceived community benefits surveillance, collaboration trust building among helped program advancement, sustainability strategies considerations. Conclusion This analysis has highlighted importance partnerships collaborations.

Language: Английский

Citations

1
Improved Passive Sampling Methods for Wastewater to Enable More Sensitive Detection of Sars-Cov-2 and its Variants DOI
Md Alamin,

Pelumi Oladipo,

James Hartrick

et al.

Published: Jan. 1, 2024

Early detection of SARS-CoV-2 in wastewater is key to mitigating the spread new outbreaks COVID-19. Wastewater-based epidemiology can surveil communities while preserving individual anonymity, especially as clinical testing for COVID-19 has decreased. However, grab samples may miss marker presence, and auto-sampling throughout a day technically challenging costly. Tampon swabs passively collect markers over hours, but recovery captured challenge. Our goal was develop more efficient tampon elution methods virus variant analysis. Counts N1 N2 were compared eluted from tampons that had been immersed 3 sewersheds 4 - 6 hr during June December 2023. Tampons either by "SwabM" adding 20 mL extraction buffer (0.5X TE +0.075% Tween-20), or "SwabM1" directly squeezed 50 syringe, "SwabM2" syringe after 2 0.5 X + 0.075% Tween-20. A subset extracted sequenced compare variants versus samples. method SwabM averaged two- three-fold higher counts than paired (p<0.0001). Eluting SwabM2 gave five-fold signals & double (both, p<0.0001) trended SwabM1 (3 5-fold grab, p<0.001). Method also quality sequences likely have enough nucleic acids sequencing. Variant analysis Fall 2023 transition XBB JN "H" lineages. Collecting with – hours improves SARS-CoV-2, potentially enabling earlier identification variants. The yields counts, detections near Limit Detection, sequences.

Language: Английский

Citations

0