Anticarcinogenic effects of water extract of sporoderm-broken spores of Ganoderma lucidum on colorectal cancer in vitro and in vivo DOI Creative Commons
Kun Na, Kang Li,

Tingting Sang

et al.

International Journal of Oncology, Journal Year: 2017, Volume and Issue: 50(5), P. 1541 - 1554

Published: March 29, 2017

Ganoderma lucidum (G. lucidum) polysaccharides (GLPs) have been used as traditional Chinese medicine for cancer prevention many years. However, the mechanism by which GLP exerts its chemopreventive activities remains elusive. In addition, it is unclear whether sporoderm-broken spores of G. water extract (BSGLWE), contains mainly GLPs, has anticancer effects on colorectal cancer. The present study investigated and potential mechanisms BSGLWE in vivo vitro. Our results showed that significantly inhibited HCT116 cell viability a time- dose-dependent manner. Flow cytometry analysis indicated disrupted cycle progression at G2/M phase via downregulation cyclin B1 A2, upregulation P21 mRNA levels. Moreover, induced apoptosis decreasing Bcl-2 survivin levels, reduced Bcl-2, PARP, pro-caspase-3 pro-caspase-9 protein Furthermore, suppressed tumor growth regulating expression genes proteins associated with apoptosis, was further confirmed reduction Ki67, PCNA, determined immunohistochemistry staining. NSAID activated gene-1 (NAG-1), pro-apoptotic gene, upregulated vitro upon treatment both relative amounts secreted NAG-1 culture medium or serum nude mice were all treatments, suggesting role BSGLWE-induced anticolorectal activity. This first to show inhibits carcinogenesis through responsible proliferation, cascades. These findings indicate possesses may serve promising agent clinical applications.

Language: Английский

A Review on Free Radicals and Antioxidants DOI
Hourieh Alkadi

Infectious Disorders - Drug Targets, Journal Year: 2018, Volume and Issue: 20(1), P. 16 - 26

Published: June 28, 2018

Free radicals are generated in our body by several systems. A balance among free and antioxidants is an important matter for appropriate physiological function. If become greater than the ability of to control them, a case known as oxidative stress appears, result that, number human diseases spread body. Antioxidants can contribute facingthis stress. The present review provides brief overview radicals, stress, some natural relationship between them.

Language: Английский

Citations

275
lncRNA H19 prevents endothelial–mesenchymal transition in diabetic retinopathy DOI Open Access
Anu Thomas, Saumik Biswas, Biao Feng

et al.

Diabetologia, Journal Year: 2019, Volume and Issue: 62(3), P. 517 - 530

Published: Jan. 5, 2019

Language: Английский

Citations

189
Zebrafish: Development of a Vertebrate Model Organism DOI Open Access
Jason R. Meyers

Current Protocols Essential Laboratory Techniques, Journal Year: 2018, Volume and Issue: 16(1)

Published: May 1, 2018

Abstract Zebrafish ( Danio rerio ) are a small‐bodied tropical, freshwater fish species originally from South Asia. The ease of care, year‐round prolific breeding, and transparent, external development have made these popular model vertebrate for many fields biology. Over the past 40 years, methods genetic developmental manipulation driven rapid rise in their use research. This review provides an orientation to zebrafish as system, its ecology life history early research adoption key development. Its current uses techniques that highlight future areas system also discussed. Finally, introduction essentials care presented, highlighting this teaching use. © 2018 by John Wiley & Sons, Inc.

Language: Английский

Citations

176
Role of polydopamine’s redox-activity on its pro-oxidant, radical-scavenging, and antimicrobial activities DOI Creative Commons
Huan Liu,

Xue Qu,

Haoqi Tan

et al.

Acta Biomaterialia, Journal Year: 2019, Volume and Issue: 88, P. 181 - 196

Published: Feb. 25, 2019

Language: Английский

Citations

172
Pesticides With Potential Thyroid Hormone-Disrupting Effects: A Review of Recent Data DOI Creative Commons
Michelle Leemans, Stephan Couderq, Barbara Demeneix

et al.

Frontiers in Endocrinology, Journal Year: 2019, Volume and Issue: 10

Published: Dec. 9, 2019

Plant Protection Products, more commonly referred to as pesticides and biocides, are used control a wide range of yield-reducing pests including insects, fungi, nematodes, weeds. Concern has been raised that some may act endocrine disrupting chemicals (EDCs) with the potential interfere hormone systems non-target invertebrates vertebrates, humans. EDCs at low doses particularly vulnerable periods exposure include pre- perinatal development. Of critical concern is number developing nervous system brain, notably thyroid signaling. Across orchestrates metamorphosis, brain development, metabolism. Pesticide action on homeostasis can involve interference TH production its control, displacement from distributor proteins liver Here we focused endpoints for each different classes reviewing epidemiological experimental studies carried out both in vivo vitro. We conclude first, many were placed market insufficient testing, other than acute or chronic toxicity, second, thyroid-specific neurodevelopmental effects mixture assessment largely absent regulatory directives.

Language: Английский

Citations

162
Integrator Recruits Protein Phosphatase 2A to Prevent Pause Release and Facilitate Transcription Termination DOI Creative Commons
Kai-Lieh Huang,

David Jee,

Chad B. Stein

et al.

Molecular Cell, Journal Year: 2020, Volume and Issue: 80(2), P. 345 - 358.e9

Published: Sept. 22, 2020

Language: Английский

Citations

155
Immunosenescence Study of T Cells: A Systematic Review DOI Creative Commons
Ivón Johanna Rodríguez,

Nicolás Lalinde Ruiz,

Manuela Llano León

et al.

Frontiers in Immunology, Journal Year: 2021, Volume and Issue: 11

Published: Jan. 15, 2021

Aging is accompanied by alterations in immune response which leads to increased susceptibility infectious diseases, cancer, autoimmunity, and inflammatory disorders. This decline function termed as immunosenescence; however, the mechanisms are not fully elucidated. Experimental approaches of adaptive immunity, particularly for T cells, have been main focus immunosenescence research. systematic review evaluates discusses cell markers implicated immunosenescence.To determine best flow cytometry circulating cells associated with immunosenescence.We systematically queried PubMed, MEDLINE, EBSCO, BVS databases original articles focused on two age groups healthy humans: 18-44 (young adults) >60 (older years. In accordance Cochrane methodology, we synthesized data through qualitative descriptions quantitative random effects meta-analysis due extensive heterogeneity.A total 36 studies conducted last 20 years were included analysis four out these used perform meta-analysis. A significant decrease naïve subset was observed older adults compared young adults. Primary identify senescent loss CD28 expression CD57 KLRG1 terminally-differentiated memory Moreover, an increase proinflammatory cytokines telomere length old adult cells. It possible synthesis markers, cytokines, because variations between groups, attributed differences protocols unreported measurements, thus generating a high risk bias.Heterogeneity among terms report, measurement techniques bias major impediments performing robust statistical that could aid identification eligible phenotype

Language: Английский

Citations

146
AGE/RAGE in diabetic kidney disease and ageing kidney DOI
Xiaqing Wu, Dandan Zhang, Yanni Wang

et al.

Free Radical Biology and Medicine, Journal Year: 2021, Volume and Issue: 171, P. 260 - 271

Published: May 18, 2021

Language: Английский

Citations

146
Effects of polysaccharides from purple sweet potatoes on immune response and gut microbiota composition in normal and cyclophosphamide treated mice DOI
Chao Tang, Jian Sun, Bo Zhou

et al.

Food & Function, Journal Year: 2017, Volume and Issue: 9(2), P. 937 - 950

Published: Dec. 13, 2017

In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble (DASP) and concentrated (CASP) were extracted from purple sweet potatoes then administered to normal cyclophosphamide (CTX) treated mice by gavage. The results showed that WSP CASP could restore the spleen index immune cytokine (IL-2 IL-6) levels in CTX mice, while DASP enhance of TNF-α, IL-2 IL-6. As compared control group, treatment groups exhibited increased Bacteroidetes, Lachnospiraceae Oscillospira, but decreased Firmicutes, Alcaligenaceae Sutterella mice. When with a model all relatively higher abundances Ruminococcaceae, Lachnospiraceae, Ruminococcus Oscillospira lower Proteobacteria, Moreover, production acetic acid, propionic acid butyric upregulate these short chain fatty acids

Language: Английский

Citations

170
The Parsortix™ Cell Separation System—A versatile liquid biopsy platform DOI Creative Commons
Michael Craig Miller,

Peggy S. Robinson,

Christopher Wagner

et al.

Cytometry Part A, Journal Year: 2018, Volume and Issue: 93(12), P. 1234 - 1239

Published: Aug. 14, 2018

Cancer cells from solid tumors can enter the circulatory system and survive to subsequently form distant metastases. The CellSearch® (Menarini-Silicon Biosystems, Huntingdon Valley, PA) was first, FDA-cleared that provided a reliable tool for investigation of circulating tumor (CTCs), which have been shown be strongly associated with poor survival therapy failure. Since time, number new technologies introduced improve CTC detection and/or isolation further characterization. continued growing interest in "liquid biopsy" field has spurred development numerous different technologies. However, selecting most appropriate platform individual applications challenging. No consensus yet reached community regarding liquid biopsy technology is optimal. Here, we introduce Parsortix™ Cell Separation System (ANGLE North America, Inc., King Prussia, PA), microfluidic based captures rare on size deformability, offers reproducibly high capture efficiency, produces highly enriched, viable (viability dependent preservative used) CTCs are amenable multitude downstream analyses, including interrogation single cells. © 2018 Authors. Cytometry Part A published by Wiley Periodicals, Inc. behalf International Society Advancement Cytometry.

Language: Английский

Citations

141