The roles of miRNA, lncRNA and circRNA in the development of osteoporosis

Biological Research, Journal Year: 2020, Volume and Issue: 53(1)

Published: Sept. 16, 2020

Abstract Osteoporosis is a common metabolic bone disease, influenced by genetic and environmental factors, that increases fragility fracture risk and, therefore, has serious adverse effect on the quality of life patients. However, epigenetic mechanisms involved in development osteoporosis remain unclear. There accumulating evidence modifications may represent underlying links factors with increased fracture. Some RNAs, such as microRNAs (miRNAs), long non-coding RNAs (lncRNAs), circular (circRNAs), have been shown to be regulators significant involvement control gene expression, affecting multiple biological processes, including metabolism. This review summarizes results recent studies miRNA-, lncRNA-, circRNA-mediated associated osteoblasts osteoclasts. Deeper insights into roles these three classes RNA could provide unique opportunities for developing novel diagnostic therapeutic approaches this disease.

Language: Английский

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Cancer drug resistance induced by EMT: novel therapeutic strategies

Archives of Toxicology, Journal Year: 2021, Volume and Issue: 95(7), P. 2279 - 2297

Published: May 18, 2021

Over the last decade, important clinical benefits have been achieved in cancer patients by using drug-targeting strategies. Nevertheless, drug resistance is still a major problem most therapies. Epithelial-mesenchymal plasticity (EMP) and tumour microenvironment described as limiting factors for effective treatment many types. Moreover, epithelial-to-mesenchymal transition (EMT) has also associated with therapy different preclinical models, although limited evidence obtained from studies samples. In this review, we particularly deepen into mechanisms of which intermediate epithelial/mesenchymal (E/M) states its interconnection to influence resistance. We describe how use bioinformatics pharmacogenomics will help figure out biological impact EMT on develop novel pharmacological approaches future.

Language: Английский

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Involvement of the long noncoding RNA H19 in osteogenic differentiation and bone regeneration

Stem Cell Research & Therapy, Journal Year: 2021, Volume and Issue: 12(1)

Published: Jan. 21, 2021

Abstract Osteogenic differentiation and bone regeneration are complex processes involving multiple genes steps. In this review, we summarize the effects of long noncoding RNA (lncRNA) H19 on osteogenic differentiation. includes matrix secretion calcium mineralization as hallmarks osteoblast absorption phosphorus osteoclast Mesenchymal stem cells (MSCs) form osteoprogenitor cells, pre-osteoblasts, mature osteoblasts, osteocytes through induction lncRNAs regulate expression coding play essential roles in regeneration. The lncRNA is known to have vital induction. This review highlights role a novel target for promotion

Language: Английский

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The Involvement of Long Non-Coding RNAs in Bone

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(8), P. 3909 - 3909

Published: April 10, 2021

A harmonious balance between osteoblast and osteoclast activity guarantees optimal bone formation resorption, pathological conditions affecting the may arise. In recent years, emerging evidence has shown that epigenetic mechanisms play an important role during osteoblastogenesis osteoclastogenesis processes, including long non-coding RNAs (lncRNAs). These molecules are a class of ncRNAs with lengths exceeding 200 nucleotides not translated into protein, have attracted attention scientific community as potential biomarkers to use for future development novel diagnostic therapeutic approaches several pathologies, diseases. This review aims provide overview lncRNAs their possible molecular in processes. The deregulation expression profiles common diseases associated altered turnover is also described. perspective, could be considered innovative help earlier diagnosis metabolism-related disorders new strategies.

Language: Английский

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Identification of mitophagy-related biomarkers in human osteoporosis based on a machine learning model

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 8, 2024

Osteoporosis (OP) is a chronic bone metabolic disease and serious global public health problem. Several studies have shown that mitophagy plays an important role in metabolism disorders; however, its osteoporosis remains unclear.

Language: Английский

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Unraveling the molecular and immunological landscape: Exploring signaling pathways in osteoporosis

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 177, P. 116954 - 116954

Published: June 20, 2024

Osteoporosis, characterized by compromised bone density and microarchitecture, represents a significant global health challenge, particularly in aging populations. This comprehensive review delves into the intricate signaling pathways implicated pathogenesis of osteoporosis, providing valuable insights pivotal role signal transduction maintaining homeostasis. The exploration encompasses cellular such as Wnt, Notch, JAK/STAT, NF-κB, TGF-β, all which play crucial roles remodeling. dysregulation these is contributing factor to necessitating profound understanding their complexities unveil molecular mechanisms underlying loss. highlights pathological significance disrupted emphasizing how deviations impact functionality osteoblasts osteoclasts, ultimately resulting heightened resorption formation. A nuanced analysis crosstalk between provided underscore relevance pathophysiology osteoporosis. Furthermore, study addresses some most long non-coding RNAs (lncRNAs) associated with adding an additional layer academic depth immune system involvement various types Finally, we propose that SKP1 can serve potential biomarker

Language: Английский

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TGF-β1 promotes epithelial-to-mesenchymal transition and stemness of prostate cancer cells by inducing PCBP1 degradation and alternative splicing of CD44

Cellular and Molecular Life Sciences, Journal Year: 2020, Volume and Issue: 78(3), P. 949 - 962

Published: May 21, 2020

Language: Английский

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Role of LncRNAs and CircRNAs in Bone Metabolism and Osteoporosis

Frontiers in Genetics, Journal Year: 2020, Volume and Issue: 11

Published: Nov. 13, 2020

Bone is a mechanosensitive organ that provides strength and support. Many bone cells, various pathways, signaling molecules coordinate the metabolism also determine course of diseases such as osteoporosis, osteonecrosis, osteopenia, etc. Osteoporosis caused by increased resorption reduced formation due to changes in level different proteins RNAs osteoclast or/and osteoblasts. The available therapeutic interventions can significantly reduce or enhance formation, but their prolonged use has deleterious side-effects. Therefore, noncoding therapeutics emerged an interesting field research. Despite advancements molecular not much known about role long circular homeostasis osteoporosis. this article, we summarize (lncRNAs) (circRNAs) cells So it might help development osteoporotic therapeutics.

Language: Английский

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lncRNA SNHG1 induced by SP1 regulates bone remodeling and angiogenesis via sponging miR-181c-5p and modulating SFRP1/Wnt signaling pathway

Molecular Medicine, Journal Year: 2021, Volume and Issue: 27(1)

Published: Nov. 3, 2021

Abstract Background We aimed to investigate the functions and underlying mechanism of lncRNA SNHG1 in bone differentiation angiogenesis development osteoporosis. Methods The differential gene or proteins expressions were measured by qPCR western blot assays, respectively. targeted relationships among molecular confirmed through luciferase reporter, RIP ChIP Alkaline phosphatase (ALP), alizarin red S (ARS) TRAP staining performed measure osteoblast/osteoclast BMSCs. viability, migration BM-EPCs validated CCK-8, clone formation, transwell tube formation Western immunofluorescence detected cytosolic/nuclear localization β-catenin. Ovariectomized (OVX) mice established confirm findings vitro. Results was enhanced miR-181c-5p decreased serum femoral tissue from OVX mice. directly inhibited activate Wnt3a/β-catenin signaling upregulating SFRP1. In addition, knockdown promoted osteogenic BMSCs increasing miR-181c-5p. contrast, overexpression advanced osteoclast BM-EPCs, whereas these effects all reversed overexpression. vivo experiments indicated that silencing alleviated osteoporosis stimulating osteoblastogenesis inhibiting osteoclastogenesis modulating Importantly, could be induced SP1 Conclusions Collectively, SP1-induced modulated SFRP1/Wnt/β-catenin pathway via sponging miR-181c-5p, thereby osteoblast while promoting formation. Further, silence might provide a potential treatment for Graphic abstract

Language: Английский

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Capsaicin Attenuates Arterial Calcification Through Promoting SIRT6-Mediated Deacetylation and Degradation of Hif1α (Hypoxic-Inducible Factor-1 Alpha)

Hypertension, Journal Year: 2022, Volume and Issue: 79(5), P. 906 - 917

Published: March 2, 2022

Sustained Hif1α (hypoxic-inducible factor-1 alpha) accumulation plays a central role in osteogenic transdifferentiation and subsequent calcification. Capsaicin, the potent agonist of TRPV1 (transient receptor potential vanilloid type 1), was found to mitigate hypoxic-related injury reverse phenotypic switch vascular smooth muscle cells. However, its arterial calcification underlying mechanisms remain unexplored.We used data from Multi-Ethnic Study Atherosclerosis examine association coronary artery chili consumption. Chronic kidney disease mice high phosphate-induced cells models were established investigate anticalcification effect capsaicin, evaluated by calcium deposition changes phenotype markers.Chili consumption negatively correlated with conferred smaller progression burden during follow-up. Capsaicin reduced both vivo vitro. Using siTRPV1 (small interfering RNA antagonist TRPV1), capsaicin abrogated. increased phosphate-treated degradation accelerated capsaicin. Retaining stability using cobalt chloride (CoCl2) or MG132 abolished protective We further identified an expression SIRT6 (Sirtuin 6) response confirmed physical interaction between Hif1α. Acetylated decreased, whereas hydroxylated under treatment. immunohistochemistry analysis, we observed transgenic capsaicin-treated chronic mice.Capsaicin facilitates deacetylation upregulating SIRT6, which inhibits protects against These highlight promising therapeutic target for management

Language: Английский

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