Exploring the Gut Microbiota–Muscle Axis in Duchenne Muscular Dystrophy

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5589 - 5589

Published: May 21, 2024

The gut microbiota plays a pivotal role in maintaining the dynamic balance of intestinal epithelial and immune cells, crucial for overall organ homeostasis. Dysfunctions these intricate relationships can lead to inflammation contribute pathogenesis various diseases. Recent findings uncovered existence gut–muscle axis, revealing how alterations disrupt regulatory mechanisms muscular adipose tissues, triggering immune-mediated inflammation. In context Duchenne dystrophy (DMD), permeability stand as potential origin molecules that could trigger muscle degeneration via pathways. Metabolites produced by bacteria, or fragments bacteria themselves, may have ability migrate from into bloodstream ultimately infiltrate distant exacerbating localized pathologies. These insights highlight alternative pathological pathways DMD beyond musculoskeletal system, paving way nutraceutical supplementation adjuvant therapy. Understanding complex interplay between microbiota, health offers new perspectives therapeutic interventions conventional approaches efficiently counteract multifaceted nature DMD.

Language: Английский

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Omega-3 polyunsatured fatty acids and physical performance across the lifespan: a narrative review

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: June 20, 2024

Background and Aims Physical performance is a major contributor of mobility independence during older life. Despite progressive decline in musculoskeletal function starts from middle age, several factors acting the life-course can negatively influence functional capacities. Lifestyle interventions incorporating nutrition physical exercise help maximizing muscle capacities early life as well preserving them later Among various dietary compounds, omega-3 polyunsaturated fatty acids (PUFAs) are gaining growing attention for their potential effects on membrane composition function. Indeed, pathways enhanced, such an attenuation pro-inflammatory oxidative stress, mitochondrial function, activation mammalian target rapamycin (mTOR) signaling reduction insulin resistance. Methods We performed narrative review to explore existing literature relationship between PUFAs across life-course. Results Growing evidence randomized controlled trials (RCTs) suggests beneficial including parameters mid On other hand, despite direct association not available literature, some mechanisms by which may contribute improved adult could be hypothesized. Conclusion Omega-3 positive effect parameters. The integration measures future studies would great interest whether starting extending throughout lifespan. However, larger high-quality RCTs needed fully elucidate supplementation mass

Language: Английский

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Differential analysis of chromatin accessibility and gene expression profiles identifies cis-regulatory elements in rat adipose and muscle

Genomics, Journal Year: 2021, Volume and Issue: 113(6), P. 3827 - 3841

Published: Sept. 20, 2021

Chromatin accessibility is a key factor influencing gene expression. We optimized the Omni-ATAC-seq protocol and used it together with RNA-seq to investigate cis-regulatory elements in rat white adipose skeletal muscle, two tissues contrasting metabolic functions. While promoter correlated RNA expression, integration of datasets identified tissue-specific differentially accessible regions (DARs) that predominantly localized intergenic intron regions. DARs were mapped expressed (DE) genes enriched distinct biological processes each tissue. Randomly selected DE validated by qPCR. Top motifs predicted binding sites for transcription factors (TFs) showing up-regulation. The correlation between differential chromatin at given TF motif expression target further supported functional relevance motif. Our study likely play major role regulation muscle.

Language: Английский

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Molecular Mechanisms and Current Treatment Options for Cancer Cachexia

Cancers, Journal Year: 2022, Volume and Issue: 14(9), P. 2107 - 2107

Published: April 23, 2022

Cancer cachexia is a condition marked by functional, metabolic, and immunological dysfunctions associated with skeletal muscle (SM) atrophy, adipose tissue loss, fat reduction, systemic inflammation, anorexia. Generally, the caused variety of mediators produced cancer cells in tumor microenvironments. Myostatin activin signaling, IGF-1/PI3K/AKT JAK-STAT signaling are known to play roles cachexia, thus, these pathways considered potential therapeutic targets. This review discusses current state knowledge molecular mechanisms underlying available options was undertaken increase understanding various factors/pathways/mediators involved identify treatment options.

Language: Английский

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Transcriptome-wide analysis of RNA m6A methylation regulation of muscle development in Queshan Black pigs

BMC Genomics, Journal Year: 2023, Volume and Issue: 24(1)

Published: May 4, 2023

Abstract Background N 6 -methyladenosine (m A) refers to the methylation modification of position RNA adenine, a dynamic reversible epigenetic that plays an important regulatory role in variety life processes. In this study, we used MeRIP-Seq and RNA-Seq longissimus dorsi (LD) muscle adult (QA) newborn (QN) Queshan Black pigs screen key genes with m A involved growth by bioinformatics analysis. Results total 23,445 25,465 peaks were found whole genomes QA QN, respectively. Among them, 613 significantly different (DMPs) 579 defined as differentially methylated (DMGs). Compared QN group, there 1,874 expressed (DEGs) including 620 up-regulated 1,254 down-regulated genes. order investigate relationship between mRNA expression at periods, combined analysis showed 88 both levels. Gene Ontology Kyoto Encyclopedia Genes Genomes results DEGs DMGs mainly skeletal tissue development, FoxO signaling pathway, MAPK insulin PI3K–Akt Wnt pathway. Four ( IGF1R , CCND2 MYOD1 FOS ) four PHKB BIN1 FUT2 ), which are closely related selected candidate for verification, consistent sequencing results, indicated reliability results. Conclusions These lay foundation understanding specific mechanisms pigs, provide theoretical references further research on development breed optimization selection.

Language: Английский

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