Endogenous and Exogenous Antioxidants in Skeletal Muscle Fatigue Development during Exercise

Antioxidants, Journal Year: 2023, Volume and Issue: 12(2), P. 501 - 501

Published: Feb. 16, 2023

Muscle fatigue is defined as a decrease in maximal force or power generated response to contractile activity, and it risk factor for the development of musculoskeletal injuries. One many stressors imposed on skeletal muscle through exercise increased production reactive oxygen species (ROS) nitrogen (RNS), which intensifies function intensity duration. Exposure ROS/RNS can affect Na+/K+-ATPase intramyofibrillar calcium turnover sensitivity, actin–myosin kinetics reduce production. On other hand, low concentrations likely upregulate an array cellular adaptative responses related mitochondrial biogenesis, glucose transport hypertrophy. Consequently, growing evidence suggests that exogenous antioxidant supplementation might hamper exercise-engendering upregulation signaling pathways mitogen-activated protein kinases (MAPKs), peroxisome-proliferator activated co-activator 1α (PGC-1α), mammalian target rapamycin (mTOR). Ultimately, both high (exercise-induced) (antioxidant intervention) ROS trigger beneficial long they do not override threshold range redox balance. The mechanisms underlying two faces exercise, well role antioxidants fatigue, are presented detail this review.

Language: Английский

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Salivary Gland Dysfunction in Stroke Patients Is Associated with Increased Protein Glycoxidation and Nitrosative Stress

Oxidative Medicine and Cellular Longevity, Journal Year: 2020, Volume and Issue: 2020, P. 1 - 14

Published: Dec. 10, 2020

Stroke is one of the leading causes disability and death worldwide. Despite intensive medical care, many complaints directly threatening patient’s life marginalize their dental needs after stroke. Recent studies indicate reduced saliva secretion in stroke patients addition to increased incidence caries periodontal disease. Since oxidative stress plays a vital role pathogenesis salivary gland hypofunction neurodegenerative disorders (including stroke), this first evaluate relationship between activity protein glycoxidation nitrosative damage. The content glycation oxidation products was assessed nonstimulated (NWS) stimulated (SWS) whole with normal hyposalivation (reduced production). study included 30 stroke’s subacute phase healthy controls matched by age sex. We have shown that show contents (↑Amadori ↑advanced end products), (↑dityrosine), nitration (↑nitrotyrosine) compared cases control group. Interestingly, higher oxidative/nitrosative found NWS, which strongly correlates flow rate, total content, amylase activity. Such relationships were not observed Summarizing, may be mechanisms responsible for impairment patients. However, extraglandular sources cannot excluded. Further assess are necessary.

Language: Английский

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Association of Tumour Microenvironment with Protein Glycooxidation, DNA Damage, and Nitrosative Stress in Colorectal Cancer

Cancer Management and Research, Journal Year: 2021, Volume and Issue: Volume 13, P. 6329 - 6348

Published: Aug. 1, 2021

In this study, we evaluated the total antioxidant capacity, nitrosative stress, and protein/DNA oxidation glycoxidation products in patients with colorectal cancer regarding histopathological parameters associated tumour microenvironment, such as inflammatory infiltration budding compare all determined between tumours located right left side of colon normal mucosa.Ferric reducing power (FRAP), stress (myeloperoxidase (MPO), nitrogen oxide (NO), peroxynitrite, nitrotyrosine), protein (protein carbonyls (PC), thiols, ischemia modified albumin (IMA)), glycooxidation (tryptophan, kynurenine, N-formylkynurenine, dityrosine, Amadori product, advanced glycation end (AGE)) 8-hydroxydeoxyguanosine (8-OHdG) were measured homogenates from cancerous tissue 30 cancer.Levels FRAP (p=0.0009), IMA (p=0.0002), kynurenine (p<0.0001), N-formylkynurenine dityrosine (p=0.0024), AGE MPO NO (p<0.0001) nitrotyrosine (p=0.0011) increased, whereas PC (p=0.0004), tryptophan 8-OHdG peroxynitrite (p=0.0003) decreased left-side compared to right-side mucosa.Our results showed that is related disturbances defense increased oxidative damages proteins DNA. These may be useful for evaluation progression differentiation location. We also demonstrated redox indicators depend on histological type influence invasion depth, presence lymph node distant metastasis, vascular neural invasion, infiltration, budding, which are part microenvironment.

Language: Английский

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A Short‐Term Resistance Training Circuit Improved Antioxidants in Sedentary Adults with Down Syndrome

Oxidative Medicine and Cellular Longevity, Journal Year: 2021, Volume and Issue: 2021(1)

Published: Jan. 1, 2021

Previous studies have found aerobic training improved oxidative damage in people with Down syndrome (DS). However, there is a lack of information regarding the influence resistance on redox imbalance this population. Accordingly, study was conducted to determine effect (RT) antioxidant defence system sedentary adults DS. Thirty‐six male DS were recruited through different community support groups. Eighteen randomly assigned perform circuit RT program 6 stations, 3 days/week for 12 weeks. Plasma total status (TAS), reduced glutathione (GHS), ascorbate, serum α ‐tocopherol, and erythrocyte reductase activity assessed. malondialdehyde (MDA) carbonyl groups (CG) assessed as markers damage. Muscle strength also measured. Dynamic torque knee extensors flexors well maximal handgrip significantly after completion program. levels TAS (GR) increased. Conversely, MDA CG reduced. It concluded Further, long‐term are required whether increased may improve clinical outcomes

Language: Английский

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Effect of Normobaric Hypoxia on Alterations in Redox Homeostasis, Nitrosative Stress, Inflammation, and Lysosomal Function following Acute Physical Exercise

Oxidative Medicine and Cellular Longevity, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 18

Published: Feb. 25, 2022

Hypoxia is a recognized inducer of oxidative stress during prolonged physical activity. Nevertheless, previous studies have not systematically examined the effects normoxia and hypoxia acute exercise. The study aimed at evaluating relationship between enzymatic nonenzymatic antioxidant barrier, total antioxidant/oxidant status, nitrosative damage, inflammation, lysosomal function in different exercise protocols under hypoxia. Fifteen competitive athletes were recruited for study. They subjected to two types cycling with intensities durations: graded until exhaustion (GE) simulated 30 km individual time trial (TT). Both performed normoxic hypoxic (FiO2 = 16.5%) conditions. number subjects was determined based on our experiment, assuming test power 0.8 α 0.05. We demonstrated enhanced systems (GE: ↑ catalase (CAT), superoxide dismutase; TT: CAT) concomitant decrease plasma reduced glutathione. In exercising hypoxia, redox status shifted favor oxidation reactions oxidant ↓ ratio), leading increased oxidation/nitration proteins advanced protein products (AOPP), ischemia-modified albumin, 3-nitrotyrosine, S-nitrosothiols; AOPP) lipids malondialdehyde). Concentrations nitric oxide its metabolites (peroxynitrite) significantly higher exercisers an associated increase inflammatory mediators myeloperoxidase, tumor necrosis factor-alpha) exoglycosidase activity N-acetyl-β-hexosaminidase, β-glucuronidase). Our indicates that even single intensive session disrupts barrier leads damage systemic level. High-intensity alters homeostasis more than less intense (TT, near anaerobic threshold) longer duration (20.2 ± 1.9 min vs. 61.1 5.4 min-normoxia; 18.0 63.7 3.0 min-hypoxia), while exacerbates stress, dysfunction athletic subjects.

Language: Английский

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Treadmill Training Effect on the Myokines Content in Skeletal Muscles of Mice With a Metabolic Disorder Model

Frontiers in Physiology, Journal Year: 2021, Volume and Issue: 12

Published: Nov. 10, 2021

The effect of treadmill training loads on the content cytokines in mice skeletal muscles with metabolic disorders induced by a 16 week high fat diet (HFD) was studied. study included accounting age and biorhythmological aspects. In experiment, were used at 4 32 weeks, end experiment-respectively 20 48 weeks. HFD feeding lasted Treadmill carried out for last weeks six times week, duration 60 min speed from 15 to 18 m/min. Three modes loading applied. first subgroup subjected stress morning hours (light phase); second evening (dark third shift mode (the first- third-weeks hours, fourth hours). 20-week-old animals, exercise does not depend regime, however, 48-week-old decrease body weight regime more profound. affected muscle myokine levels. all myokines, except LIF, decreased, while concentration CLCX1 decreased only young animals response HFD. caused multidirectional changes myokines tissue. IL-6 changed most profoundly. These observed groups animals. depended greatest extent time scheme. physical activity IL-15 tissue mostly mice. led an increase LIF when applied under during dark phase or group, this significantly pronounced. CXCL1 did change use almost Physical activity, introduced considering circadian rhythms, is promising way influencing processes both cellular systemic levels, which important search new ways correcting disorders.

Language: Английский

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Cross-Talk Between Nitrosative Stress, Inflammation and Hypoxia-Inducible Factor in Patients with Adrenal Masses

Journal of Inflammation Research, Journal Year: 2021, Volume and Issue: Volume 14, P. 6317 - 6330

Published: Nov. 1, 2021

Adrenal masses are the most common of all human tumors. The role nitrosative stress and inflammation in cancer development has already been demonstrated. However, it is not known whether they involved pathogenesis adrenal aim study was to investigate a cross-talk between stress, hypoxia-inducible factor (HIF-1α) 75 patients with different types (non-functional incidentaloma, pheochromocytoma Cushing's/Conn's adenoma).The plasma concentrations total nitric oxide (NO), S-nitrosothiols, peroxynitrite nitrotyrosine activity serum myeloperoxidase (MPO) were measured spectrophotometrically, whereas interleukin 1 beta (IL-1β), tumor necrosis α (TNF-α) alpha using commercial ELISA kits. control group consisted 50 healthy people matched by age sex group. number subjects determined priori based on our previous experiment (power test = 0.9; 0.05).We found significantly higher (↑nitric oxide, ↑peroxynitrite, ↑S-nitrosothiols ↑nitrotyrosine) tumors, which accompanied increased inflammatory (↑myeloperoxidase, ↑interleukin ↑tumor α) hypoxia biomarkers. Peroxynitrite positively correlated aldosterone level. Nitrosative also associated HIF-1α. Interestingly, MPO differentiated from individuals high sensitivity specificity. Moreover, multivariate regression analysis, we showed that ONOO- IL-1β depended cortisol level, while ONOO-, HIF-1α aldosterone. Unfortunately, none assessed biomarkers studied, suggesting severity damage similar pheochromocytoma, Cushing's or Conn's adenoma.Adrenal tumors protein nitration/S-nitrosylation inflammation.

Language: Английский

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Aerobic training associated with an active lifestyle exerts a protective effect against oxidative damage in hypothalamus and liver: The involvement of energy metabolism

Brain Research Bulletin, Journal Year: 2021, Volume and Issue: 175, P. 116 - 129

Published: July 22, 2021

Language: Английский

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Redox Status Response of Physical Exercise Training in Women with Breast Cancer during Trastuzumab Therapy

Healthcare, Journal Year: 2022, Volume and Issue: 10(10), P. 2039 - 2039

Published: Oct. 15, 2022

Trastuzumab is indicated in the adjuvant setting for early and intermediate stages of breast cancer (BC) positive epidermal growth factor receptor 2 (HER2). Although HER2 BC patients tends to disrupt pro-oxidant inflammatory signaling, influence trastuzumab modulating this process remains unknown. Due absence any chemotherapeutic or chemoprophylactic agents trastuzumab-induced side effects, study investigated potential role regular physical exercise antioxidant defenses, oxidative stress, nitrosative damage during treatment.The aimed analyze relationship between activity redox status women with therapy.We observed 50 therapy two groups: one that undertook moderately intensive supervised exercises, a second performed according recommendations patients, along third (control) group healthy women.The enzyme non-enzymatic activities were significantly higher exercised compared other participants. The concentrations lipid protein stress products both groups than controls.Trastuzumab treatment stimulates response patients. results highlight imbalance parallel training therapy. Further studies are needed different intensities levels treatment.

Language: Английский

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Treadmill running on neuropathic pain: via modulation of neuroinflammation

Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17

Published: June 26, 2024

Neuropathic pain is a type of chronic caused by an injury or somatosensory nervous system disease. Drugs and exercise could effectively relieve neuropathic pain, but no treatment can completely stop pain. The integration into management has attracted considerable interest in recent years, treadmill training the most used among therapies. be treated if its mechanism clarified. In association between neuroinflammation been explored. Neuroinflammation trigger proinflammatory cytokines, activate microglia, inhibit descending modulatory systems, promote overexpression brain-derived neurotrophic factor, which lead to generation hypersensitivity. Treadmill alleviate mainly regulating neuroinflammation, including inhibiting activity pro-inflammatory factors over activation microglia dorsal horn, expression mu opioid receptor rostral ventromedial medulla levels γ-aminobutyric acid factor. This article reviews summarizes research on effect role regulation explore benefits for treatment.

Language: Английский

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