Milk exosomes-mediated miR-31-5p delivery accelerates diabetic wound healing through promoting angiogenesis

Drug Delivery, Journal Year: 2022, Volume and Issue: 29(1), P. 214 - 228

Published: Jan. 5, 2022

The refractory diabetic wound has remained a worldwide challenge as one of the major health problems. impaired angiogenesis phase during healing partly contributes to pathological process. MicroRNA (miRNA) is an essential regulator gene expression in crucial biological processes and promising nucleic acid drug therapeutic fields wound. However, miRNA therapies have limitations due lacking effective delivery system. In present study, we found significant reduction miR-31-5p full-thickness wounds mice compared normal mice. Further, been proven promote proliferation, migration, endothelial cells. Thus, conceived idea exogenously supplementing mimics treat We used milk-derived exosomes novel system for successfully encapsulated into milk through electroporation. Then, proved that loaded achieved higher cell uptake was able resist degradation. Moreover, our miRNA-exosomal formulation demonstrated dramatically improved functions vitro, together with promotion enhanced vivo. Collectively, data showed feasibility scalable, biocompatible, cost-effective enhance bioavailability efficacy miRNAs.

Language: Английский

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High-quality milk exosomes as oral drug delivery system

Biomaterials, Journal Year: 2021, Volume and Issue: 277, P. 121126 - 121126

Published: Sept. 9, 2021

Language: Английский

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Exosome-Derived MicroRNAs of Human Milk and Their Effects on Infant Health and Development

Biomolecules, Journal Year: 2021, Volume and Issue: 11(6), P. 851 - 851

Published: June 7, 2021

Multiple biologically active components of human milk support infant growth, health and development. Milk provides a wide spectrum mammary epithelial cell-derived extracellular vesicles (MEVs) for the infant. Although whole MEVs appears to be functional importance growing infant, majority recent studies report on MEV subfraction exosomes (MEX) their miRNA cargo, which are in focus this review. MEX dominant miRNA-148a play key role intestinal maturation, barrier function suppression nuclear factor-κB (NF-κB) signaling may thus helpful prevention treatment necrotizing enterocolitis. miRNAs reach systemic circulation impact epigenetic programming various organs including liver, thymus, brain, pancreatic islets, beige, brown white adipose tissue as well bones. Translational evidence indicates that control expression global cellular regulators such DNA methyltransferase 1-which is important up-regulation developmental genes insulin, insulin-like growth factor-1, α-synuclein forkhead box P3-and receptor-interacting protein 140, regulation multiple receptors. MEX-derived miRNA-30b stimulate uncoupling 1, inducer thermogenesis converting into beige/brown tissue. have considered signalosomes derived from maternal lactation genome emitted promote immunological metabolic offspring. Deeper insights milk's molecular biology allow conclusion infants both "breast-fed" "breast-programmed". In regard, miRNA-deficient artificial formula not an adequate substitute breastfeeding, birthright all mammals.

Language: Английский

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Extracellular vesicles and particles impact the systemic landscape of cancer

The EMBO Journal, Journal Year: 2022, Volume and Issue: 41(18)

Published: Sept. 2, 2022

Language: Английский

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Engineering Extracellular Vesicles as Delivery Systems in Therapeutic Applications

Advanced Science, Journal Year: 2023, Volume and Issue: 10(17)

Published: April 20, 2023

Extracellular vesicles (EVs) are transport secreted by living cells and released into the extracellular environment. Recent studies have shown that EVs serve as "messengers" in intercellular inter-organismal communication, both normal pathological processes. EVs, natural nanocarriers, can deliver bioactivators therapy with their endogenous properties. This review article describes engineering of sources, isolation method, cargo loading, boosting approach, adjustable targeting EVs. Furthermore, summarizes recent progress made EV-based delivery systems applications, including cancer, cardiovascular diseases, liver, kidney, nervous system COVID-19 emphasizes obstacles challenges therapies possible strategies.

Language: Английский

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Tumor-targeted exosomes for delivery of anticancer drugs

Cancer Letters, Journal Year: 2023, Volume and Issue: 558, P. 216093 - 216093

Published: Feb. 22, 2023

Exosomes are small phospholipid bilayer vesicles that naturally produced by all living cells, both prokaryotes and eukaryotes. The exosomes due to their unique size, reduced immunogenicity, ability mimic synthetic liposomes in carrying various anticancer drugs have been tested as drug delivery vehicles for cancer treatment. An added advantage of developing a carrier is the ease manipulating intraluminal content surface modification achieve tumor-targeted delivery. In past ten-years, there has an exponential increase number exosome-related studies cancer. Preclinical demonstrate exosomes-mediated chemotherapeutics, biologicals natural products produce potent activity both, vitro vivo. contrast, exosome-based clinical trials few challenges manufacturing scalability related large-scale production storage stability. Herein, we discuss recent advances treatment preclinical conclude with be overcome translating larger therapies into clinic.

Language: Английский

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Biogenesis, Isolation, and Detection of Exosomes and Their Potential in Therapeutics and Diagnostics

Biosensors, Journal Year: 2023, Volume and Issue: 13(8), P. 802 - 802

Published: Aug. 10, 2023

The increasing research and rapid developments in the field of exosomes provide insights into their role significance human health. Exosomes derived from various sources, such as mesenchymal stem cells, cardiac tumor to name a few, can be potential therapeutic agents for treatment diseases could also serve biomarkers early detection diseases. Cellular components exosomes, several proteins, lipids, miRNAs hold promise novel structure enables them drug delivery vehicles. Since exhibit applications, efficient isolation complex biological/clinical samples precise real-time analysis becomes significant. With advent microfluidics, nano-biosensors are being designed capture efficiently rapidly. Herein, we have summarized history, biogenesis, characteristics, functions, applications along with isolation, detection, quantification techniques. implications surface modifications enhance specificity been outlined. review sheds light on engineered nanoplatforms developed exosome capture.

Language: Английский

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The emerging role of exosomes in innate immunity, diagnosis and therapy

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 16, 2023

Exosomes, which are nano-sized transport bio-vehicles, play a pivotal role in maintaining homeostasis by exchanging genetic or metabolic information between different cells. Exosomes can also vital transferring virulent factors the host and parasite, thereby regulating gene expression immune interphase. The association of inflammation with disease development potential exosomes to enhance mitigate inflammatory pathways support notion that have alter course disease. Clinical trials exploring cancer, osteoporosis, renal, neurological, pulmonary disorders currently underway. Notably, available on signatory efficacy immune-related remains elusive sporadic. In this review, we discuss cell-derived their application immunotherapy, including those against autoimmune connective tissue diseases. Further, elucidated our views major issues pathophysiological processes. Therefore, presented review highlights as promising strategies clinical tools for regulation.

Language: Английский

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Therapeutic potential of plant-derived extracellular vesicles as nanocarriers for exogenous miRNAs

Pharmacological Research, Journal Year: 2023, Volume and Issue: 198, P. 106999 - 106999

Published: Nov. 19, 2023

Cell-to-cell communication strategies include extracellular vesicles (EVs) in plants and animals. The bioactive molecules a diet rich vegetables fruits are associated with disease-preventive effects. Plant-derived EVs (PDEVs) biogenetically morphologically comparable to mammalian transport molecules, including miRNAs. However, the biological functions of PDEVs not fully understood, standard isolation protocols lacking. Here, were isolated from four foods combination ultracentrifugation size exclusion chromatography, evaluated as vehicles for enhanced synthetic In addition, role food-derived carriers dietary (poly)phenols other secondary metabolites was investigated. broccoli, pomegranate, apple, orange efficiently characterized. all sources, 4 miRNA families present tissues EVs. miRNAs broccoli fruit-derived showed reduced RNase degradation ferried inside exposed cells. transfected ath-miR159a, ath-miR162a-3p, ath-miR166b-3p, ath-miR396b-5p toxic effects on human cells, did natural alone. trace amounts phytochemicals, flavonoids, anthocyanidins, phenolic acids, or glucosinolates. Thus, can act nanocarriers functional that could be used RNA-based therapy.

Language: Английский

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Exosomes: a review of biologic function, diagnostic and targeted therapy applications, and clinical trials

Journal of Biomedical Science, Journal Year: 2024, Volume and Issue: 31(1)

Published: July 11, 2024

Abstract Exosomes are extracellular vesicles generated by all cells and they carry nucleic acids, proteins, lipids, metabolites. They mediate the exchange of substances between cells,thereby affecting biological properties activities recipient cells. In this review, we briefly discuss composition exocomes exosome isolation. We also review clinical applications exosomes in cancer biology as well strategies exosome-mediated targeted drug delivery systems. Finally, application context therapeutics both practice literature discussed.

Language: Английский

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