Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 249, P. 114506 - 114506
Published: Jan. 15, 2025
Language: Английский
Cell Communication and Signaling, Journal Year: 2023, Volume and Issue: 21(1)
Published: Jan. 23, 2023
Abstract MicroRNAs (miRNAs) are a group of small non-coding RNAs that regulate gene expression by targeting mRNA. Moreover, it has been shown miRNAs changed in various diseases, such as cancers, autoimmune disease, infectious and neurodegenerative Diseases. The suppression miRNA function can be easily attained utilizing anti-miRNAs. In contrast, an enhancement achieved through the utilization modified mimetics. discovery appropriate carriers body become interesting subject for investigators. Exosomes (EXOs) therapeutic efficiency safety transferring different cellular biological components to recipient cell have attracted significant attention their capability carriers. Mesenchymal stem cells (MSCs) recognized generate wide range EXOs (MSC-EXOs), showing MSCs may effective EXO generation clinically measure compared other origins. MSC-EXOs widely investigated because immune attributes, tumor-homing flexible characteristics. this article, we summarized features MSC-EXOs, including production, purification, loading methods modification targeted delivery diseases. Graphical abstract
Language: Английский
Citations
78
Advanced Science, Journal Year: 2023, Volume and Issue: 10(17)
Published: April 20, 2023
Extracellular vesicles (EVs) are transport secreted by living cells and released into the extracellular environment. Recent studies have shown that EVs serve as "messengers" in intercellular inter-organismal communication, both normal pathological processes. EVs, natural nanocarriers, can deliver bioactivators therapy with their endogenous properties. This review article describes engineering of sources, isolation method, cargo loading, boosting approach, adjustable targeting EVs. Furthermore, summarizes recent progress made EV-based delivery systems applications, including cancer, cardiovascular diseases, liver, kidney, nervous system COVID-19 emphasizes obstacles challenges therapies possible strategies.
Language: Английский
Citations
62
International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 7923 - 7940
Published: Dec. 1, 2023
Exosomes are nano-sized membrane vesicles that transfer bioactive molecules between cells and modulate various biological processes under physiological pathological conditions. By applying bioengineering technologies, exosomes can be modified to express specific markers or carry therapeutic cargo emerge as novel platforms for the treatment of cancer, neurological, cardiovascular, immune, infectious diseases. However, there many challenges uncertainties in clinical translation exosomes. This review aims provide an overview recent advances engineered exosomes, with a special focus on methods strategies loading drugs into pros cons different methods, optimization exosome production based encapsulated. Moreover, we also summarize current applications prospects well potential risks limitations need addressed engineering, including standardization preparation engineering protocols, quality quantity control drug release, immunogenicity cytotoxicity Overall, represent exciting frontier nanomedicine, but they still face large-scale production, maintenance storage stability, translation. With continuous this field, exosome-based formulation could offer great promise targeted human
Language: Английский
Citations
59
International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 1537 - 1560
Published: March 1, 2023
Abstract: Diabetes mellitus (DM) induced wound healing impairment remains a serious health problem and burden on the clinical obligation for high amputation rates. Based features of microenvironment, biomaterials loading specific drugs can benefit diabetic treatment. Drug delivery systems (DDSs) carry diverse functional substances to site. Nano-drug (NDDSs), benefiting from their related nano size, overcome limitations conventional DDSs application are considered as developing process in treatment field. Recently, number finely designed nanocarriers efficiently various (bioactive non-bioactive factors) have emerged circumvent constraints faced by traditional DDSs. This review describes recent advances nano-drug involved mitigating diabetes mellitus-based non-healing wounds. Keywords: drug system, nanotechnology, healing, nanoparticles
Language: Английский
Citations
49
Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 356, P. 463 - 480
Published: March 15, 2023
Complexity and difficulties in wound management are pressing concerns that affect patients' quality of life may result tissue infection, necrosis, loss local systemic functions. Hence, novel approaches to accelerate healing being actively explored over the last decade. Exosomes as important mediators intercellular communications promising natural nanocarriers due their biocompatibility, low immunogenicity, drug loading targeting capacities, innate stability. More importantly, exosomes developed a versatile pharmaceutical engineering platform for repair. This review provides an overview biological physiological functions derived from variety origins during phases, strategies exosomal engineering, therapeutic applications skin regeneration.
Language: Английский
Citations
48
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: Jan. 3, 2024
Abstract Exosomes are nanoscale extracellular vesicles secreted by cells and enclosed a lipid bilayer membrane containing various biologically active cargoes such as proteins, lipids, nucleic acids. Engineered exosomes generated through genetic modification of parent show promise drug delivery vehicles, they have been demonstrated to great therapeutic potential for treating cancer, cardiovascular, neurological, immune diseases, but systematic knowledge is lacking regarding optimization loading assessment efficacy. This review summarizes current approaches engineering evaluating their effects, techniques assessing exosome release kinetics, cell targeting, biodistribution, pharmacokinetics, outcomes critically examined. Additionally, this synthesizes the latest applications in clinical translation. The compiled provides framework rational design rigorous therapeutics. Continued advancement robust characterization methods reporting standards will accelerate development technologies pave way studies. Graphical
Language: Английский
Citations
40
Bioactive Materials, Journal Year: 2024, Volume and Issue: 36, P. 126 - 156
Published: March 2, 2024
Small extracellular vesicles (sEVs) are known to be secreted by a vast majority of cells. These sEVs, specifically exosomes, induce specific cell-to-cell interactions and can activate signaling pathways in recipient cells through fusion or interaction. nanovesicles possess several desirable properties, making them ideal for regenerative medicine nanomedicine applications. properties include exceptional stability, biocompatibility, wide biodistribution, minimal immunogenicity. However, the practical utilization particularly clinical settings at large scale, is hindered expensive procedures required their isolation, limited circulation lifetime, suboptimal targeting capacity. Despite these challenges, sEVs have demonstrated remarkable ability accommodate various cargoes found extensive applications biomedical sciences. To overcome limitations broaden potential applications, researchers should strive deepen understanding current loading, characterization techniques. Additionally, acquiring fundamental knowledge about origins employing state-of-the-art methodologies expand research scope. This review provides comprehensive overview exosome-based strategies diverse domains, encompassing cancer therapy, immunotherapy, biomarker Furthermore, we emphasize immense exosomes medicine.
Language: Английский
Citations
32
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: July 5, 2024
Abstract Diabetic wounds are characterized by incomplete healing and delayed healing, resulting in a considerable global health care burden. Exosomes lipid bilayer structures secreted nearly all cells express characteristic conserved proteins parent cell-associated proteins. harbor diverse range of biologically active macromolecules small molecules that can act as messengers between different cells, triggering functional changes recipient thus endowing the ability to cure various diseases, including diabetic wounds. accelerate wound regulating cellular function, inhibiting oxidative stress damage, suppressing inflammatory response, promoting vascular regeneration, accelerating epithelial facilitating collagen remodeling, reducing scarring. from tissues or potentially possess functions varying levels promote healing. For example, mesenchymal stem cell-derived exosomes (MSC-exos) have favorable potential field due their superior stability, permeability, biocompatibility, immunomodulatory properties. Exosomes, which derived skin components, modulate inflammation regeneration key turn promotes Therefore, this review mainly emphasizes roles mechanisms sources, represented MSCs improving A deeper understanding therapeutic will yield promising candidates perspectives for management.
Language: Английский
Citations
24
Frontiers in Genetics, Journal Year: 2022, Volume and Issue: 13
Published: Oct. 21, 2022
Exosomes (EXOs) are natural nanoparticles of endosome origin that secreted by a variety cells in the body. have been found bio-fluids such as urine, saliva, amniotic fluid, and ascites, among others. Milk is only commercially available biological liquid containing EXOs. Proof exosomes essential for cell-to-cell communication increasingly being reported. Studies shown they migrate from cell to various bioactive substances, including membrane receptors, proteins, mRNAs, microRNAs, organelles, or can stimulate target directly through interactions with receptors. Because presence specific lipids, RNAs, act physiological pathological conditions vivo . Other salient features EXOs include their long half-life body, no tumorigenesis, low immune response, good biocompatibility, ability surface biomarkers, capacity carry macromolecules. introduced scientific community important, efficient, attractive nanoparticles. They be extracted different sources same characteristics parents. present milk separated size exclusion chromatography, density gradient centrifugation, (ultra) centrifugation; however, complex composition includes casein micelles fat globules makes it necessary take additional issues into consideration when employing mentioned techniques milk. As rich source EXOs, has unique properties that, addition its role carrier, promotes use treating diseases digestive problems, skin ulcers, cancer, Moreover, derived camel reported reduce risk oxidative stress cancer. Milk-derived (MDEs) yak improves gastrointestinal tract (GIT) development under hypoxic conditions. Furthermore, yak-MDEs suggested best treatment intestinal epithelial (IEC-6 line). availability well non-invasiveness cost-effectiveness preparation, isolates mammals excellent resources studies related These also make possible exploit MDEs clinical trials. The current study aimed investigate therapeutic applications isolated sources.
Language: Английский
Citations
60
Asian Journal of Pharmaceutical Sciences, Journal Year: 2022, Volume and Issue: 18(1), P. 100772 - 100772
Published: Dec. 31, 2022
In the inflammatory microenvironment, there are numerous exosomes secreted by immune cells (Macrophages, neutrophils, dendritic cells), mesenchymal stem (MSCs) and platelets as intercellular communicators, which participate in regulation of inflammation modulating gene expression releasing anti-inflammatory factors. Due to their good biocompatibility, accurate targeting, low toxicity immunogenicity, these able selectively deliver therapeutic drugs site through interactions between surface-antibody or modified ligand with cell surface receptors. Therefore, role exosome-based biomimetic delivery strategies diseases has attracted increasing attention. Here we review current knowledge techniques for exosome identification, isolation, modification drug loading. More importantly, highlight progress using treat chronic such rheumatoid arthritis (RA), osteoarthritis (OA), atherosclerosis (AS), bowel disease (IBD). Finally, also discuss potential challenges carriers.
Language: Английский
Citations
52