Pharmaceuticals,
Journal Year:
2025,
Volume and Issue:
18(4), P. 508 - 508
Published: March 31, 2025
Background:
Alchornea
laxiflora
(Benth.)
Pax
&
K.
Hoffm.
(A.
laxiflora)
is
utilized
as
a
traditional
herb
for
treating
several
diseases.
Objective:
Our
study
aims
to
identify
the
active
phytochemical
candidates
from
A.
and
analyses
predict
their
anticancer
activity
mechanism
by
employing
network
pharmacology,
molecular
docking,
dynamics
(MD).
Methods:
The
phytoconstituents
of
were
retrieved
literature,
phytoconstituent-related
targets
implicated
in
hepatocellular
carcinoma
(HCC)
collected
respective
databases.
Computational
methods
employed
recognize
essential
compounds,
hub
gene
targets,
signaling
pathways.
Results:
has
identified
12
potentially
bioactive
150
potential
anti-HCC
15
laxiflora.
Molecular
docking
results
recognized
better
binding
energy
values
below
−5.6
kcal/mol.
Further,
MD
simulations
three
top-scoring
protein–ligand
complexes
(MAPK—3-acetylursolic
acid,
AKT1—quercetin,
AKT1—3-acetylursolic
acid)
allowed
us
validate
results,
evaluate
stability
complexes,
associated
conformational
changes.
Conclusions:
research
claims
that
are
crucial
liver
cancer,
protein
can
serve
biomarkers,
respectively.
Journal of Cellular and Molecular Medicine,
Journal Year:
2021,
Volume and Issue:
26(2), P. 287 - 305
Published: Dec. 14, 2021
Abstract
Hepatocellular
carcinoma
(HCC),
as
the
most
prevalent
liver
malignancy,
is
annually
diagnosed
in
more
than
half
a
million
people
worldwide.
HCC
strongly
associated
with
hepatitis
B
and
C
viral
infections
well
alcohol
abuse.
Obesity
nonalcoholic
fatty
disease
(NAFLD)
also
significantly
enhance
risk
of
cancer.
Despite
recent
improvements
therapeutic
approaches,
patients
advanced
stages
show
poor
prognosis.
Accumulating
evidence
provides
support
for
regulatory
role
non‐coding
RNAs
(ncRNAs)
There
are
variety
reports
indicating
microRNAs
(miRNAs)
different
HCC.
Long
(LncRNAs)
exert
their
effects
by
sponging
miRNAs
controlling
expression
miRNA‐targeted
genes.
Circular
(circRNAs)
perform
biological
functions
acting
transcriptional
regulators,
miRNA
sponges
protein
templates.
Diverse
studies
have
illustrated
that
dysregulation
competing
endogenous
RNA
networks
(ceRNETs)
remarkably
correlated
HCC‐causing
diseases
such
chronic
infections,
steatohepatitis
fibrosis/cirrhosis.
The
aim
current
article
was
to
provide
an
overview
molecular
mechanisms
underlying
function
ceRNETs
modulate
characteristics
uncontrolled
cell
proliferation,
resistance
death,
metabolic
reprogramming,
immune
escape,
angiogenesis
metastasis.
knowledge
highlights
potential
these
molecules
novel
diagnostic
biomarkers
targets
Metabolites,
Journal Year:
2023,
Volume and Issue:
13(10), P. 1047 - 1047
Published: Oct. 2, 2023
Hepatocellular
carcinoma
(HCC),
the
most
prevalent
form
of
liver
cancer,
is
third
leading
cause
mortality
globally.
Patients
with
HCC
have
a
poor
prognosis
due
to
fact
that
emergence
symptoms
typically
occurs
at
late
stage
disease.
In
addition,
conventional
biomarkers
perform
suboptimally
when
identifying
in
its
early
stages,
heightening
need
for
identification
new
and
more
effective
biomarkers.
Using
metabolomics
lipidomics
approaches,
this
study
aims
identify
serum
patients
cirrhosis
(LC).
Serum
samples
from
20
cases
LC
were
analyzed
using
ultra-high-performance
liquid
chromatography-Q
Exactive
mass
spectrometry
(UHPLC-Q-Exactive-MS).
Metabolites
lipids
are
significantly
altered
between
identified.
These
include
organic
acids,
amino
TCA
cycle
intermediates,
fatty
bile
glycerophospholipids,
sphingolipids,
glycerolipids.
The
significant
variability
was
observed
concentrations
glycerophospholipids.
context
cases,
there
notable
increase
levels
phosphatidylethanolamine
triglycerides,
but
acids
phosphatidylcholine
exhibited
substantial
decrease.
it
all
identified
metabolites
superior
area
under
receiver
operating
characteristic
(ROC)
curve
comparison
alpha-fetoprotein
(AFP).
pathway
analysis
these
revealed
acid,
lipid,
energy
metabolism
as
impacted
pathways.
Putative
will
be
validated
future
studies
via
targeted
quantification.
OncoTargets and Therapy,
Journal Year:
2022,
Volume and Issue:
Volume 15, P. 291 - 298
Published: March 1, 2022
Hepatocellular
carcinoma
(HCC)
is
one
of
the
deadliest
cancers
in
world
with
a
five-year
survival
rate
less
than
20%.
Nonetheless,
selecting
an
appropriate
therapeutic
agent
to
inhibit
development
hepatoma
cells
still
challenge.
Bufalin,
component
traditional
Chinese
medicine
Chansu,
has
been
shown
proliferation,
invasion
and
metastasis
HCC
through
various
signaling
pathways.
In
addition,
bufalin
sorafenib
demonstrate
synergistic
effect
cancer
therapeutics.
This
review
highlighted
on
several
focal
pathways
involved
inhibitory
effects
its
mechanisms
sorafenib.
The
immunotherapy
also
discussed
as
novel
property.
Life,
Journal Year:
2023,
Volume and Issue:
13(7), P. 1532 - 1532
Published: July 10, 2023
AKT1
is
a
serine/threonine
kinase
necessary
for
the
mediation
of
apoptosis,
angiogenesis,
metabolism,
and
cell
proliferation
in
both
normal
cancerous
cells.
The
mutations
gene
have
been
associated
with
different
types
cancer.
Further,
are
also
reported
to
be
other
diseases
such
as
Proteus
syndrome
Cowden
syndromes.
Hence,
this
study
aims
identify
deleterious
missense
SNPs
predict
their
effect
on
function
structure
protein
using
various
computational
tools.Extensive
silico
approaches
were
applied
human
assessment
impact
protein.
association
these
highly
forms
cancers
was
analyzed.
approach
can
help
reducing
cost
time
required
diseases.In
study,
12
identified
which
could
affect
Out
12,
four
SNPs-namely,
G157R,
G159V,
G336D,
H265Y-were
predicted
located
at
conserved
residues.
G157R
ligand
binding
Another
SNP,
R273Q,
liver
cancer.This
useful
pharmacogenomics,
molecular
diagnosis
diseases,
developing
inhibitors
oncogene.
Cancers,
Journal Year:
2022,
Volume and Issue:
14(18), P. 4524 - 4524
Published: Sept. 19, 2022
Circular
RNAs
(circRNAs)
have
been
shown
to
play
an
important
role
in
the
pathogenesis
of
hepatocellular
carcinoma
(HCC).
By
implementing
available
transcriptomic
analyses
HCC
patients,
we
identified
upregulated
circRNA
hsa_circ_0062682.
Stable
perturbations
hsa_circ_0062682
Huh-7
and
SNU-449
cell
lines
influenced
colony
formation,
migration,
proliferation,
sorafenib
sensitivity,
additionally
induced
morphological
changes
lines,
indicating
oncogenesis.
Pathway
enrichment
analysis
gene
set
transcriptome
data
from
knockdown
explained
observed
phenotypes
exposed
transcription
factors
E2F1,
Sp1,
HIF-1α,
NFκB1
as
potential
downstream
targets.
Biotinylated
oligonucleotide
pulldown
combined
with
proteomic
protein
interaction
partners
which
YBX1,
a
known
oncogene,
was
confirmed
by
RNA
immunoprecipitation.
Furthermore,
discovered
complex
cell-type-specific
phenotype
response
oncogenic
This
finding
is
line
different
classes
tumours,
more
studies
are
needed
shed
light
on
molecular
complexity
liver
cancer.
Biomedicines,
Journal Year:
2021,
Volume and Issue:
9(11), P. 1527 - 1527
Published: Oct. 24, 2021
Trytanthrin,
found
in
Ban-Lan-Gen,
is
a
natural
product
containing
an
indoloquinazoline
moiety
and
has
been
shown
to
possess
anti-inflammatory
anti-viral
activities.
Chronic
inflammation
hepatitis
B
are
known
be
associated
with
the
progression
of
hepatocellular
carcinoma
(HCC).
In
this
study,
series
tryptanthrin
derivatives
were
synthesized
generate
potent
anti-tumor
agents
against
HCC.
This
effort
yielded
two
compounds,
A1
A6,
that
exhibited
multi-fold
higher
cytotoxicity
HCC
cells
than
parent
compound.
Flow
cytometric
analysis
demonstrated
A6
caused
S-phase
arrest
downregulated
expression
cyclin
A1,
B1,
CDK2,
p-CDC2.
addition
inducing
caspase-dependent
apoptosis,
similar
regulation
phosphorylation
or
multiple
signaling
targets,
including
Akt,
NF-κB,
mitogen-activated
protein
kinases.
The
activities
also
attributable
generation
reactive
oxygen
species,
accompanied
by
increase
p-p53
levels.
Therefore,
have
potential
clinical
applications
since
they
target
diverse
aspects
cancer
cell
growth
Carcinogenesis,
Journal Year:
2023,
Volume and Issue:
44(10-11), P. 748 - 759
Published: Sept. 22, 2023
Copine
1
(CPNE1),
a
membrane-binding
protein,
influences
the
prognosis
of
various
cancers.
According
to
cBioPortal,
CPNE1
amplification
is
prevalent
genetic
mutation
in
ovarian
cancer
but
with
unknown
oncogenic
mechanism.
International Journal of Molecular Sciences,
Journal Year:
2022,
Volume and Issue:
23(24), P. 16206 - 16206
Published: Dec. 19, 2022
Hepatocellular
carcinoma
(HCC)
is
one
of
the
leading
causes
cancer-related
mortality
worldwide.
The
AKT
pathway
often
activated
in
HCC
cases,
and
a
longer
exposure
to
tyrosine
kinase
inhibitors
such
as
sorafenib
may
lead
over-activation
pathway,
resistance.
Here,
we
studied
efficacy
new
generation
allosteric
inhibitor,
vevorisertib,
alone
or
combination
with
sorafenib.
To
identify
specific
adverse
effects
related
background
cirrhosis,
used
diethylnitrosamine
(DEN)-induced
cirrhotic
rat
model.
Vevorisertib
was
tested
vitro
on
Hep3B,
HepG2,
HuH7
PLC/PRF
cell
lines.
Rats
were
treated
weekly
intra-peritoneal
injections
DEN
for
14
weeks
obtain
cirrhosis
fully
developed
HCC.
After
that,
rats
randomized
into
four
groups
(n
=
7/group):
control,
sorafenib,
vevorisertib
+
6
weeks.
Tumor
progression
followed
by
MRI.
We
demonstrated
that
highly
potent
treatment,
blocking
phosphorylation
AKT.
tumor
liver
significantly
reduced
treatment
(49.4%)
compared
control
group
(158.8%,
p
<
0.0001).
size,
number
proliferation
both
group.
Sirius
red
staining
showed
an
improvement
fibrosis
treatment.
Moreover,
associated
normalization
vasculature.
Altogether,
single
agent
its
exerted
strong
suppression
improved
fibrosis.
Thus,
results
provide
rationale
testing
clinical
settings
confirm
importance
targeting
