Contrast-Enhancing Staining Agents for ex vivo Contrast-Enhanced Computed Tomography: a review

Tomography of Materials and Structures, Journal Year: 2025, Volume and Issue: 7, P. 100052 - 100052

Published: Feb. 1, 2025

Language: Английский

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Quantitative susceptibility mapping of the human carotid artery: Assessing sensitivity to elastin and collagen ex vivo

Magnetic Resonance in Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Abstract Purpose The aim is to establish the relationship between carotid susceptibility and microstructural components in diseased arteries. Methods Excised cadaveric arteries ( n = 5) were scanned using high‐resolution QSM at 7 Tesla. After ex vivo imaging, all samples brought histology stained for elastin, collagen, cells, calcium. An image registration pipeline was used combination with semi‐quantitative, regional analysis evaluate relationships MRI components. Results Weak, non‐significant p > 0.05) correlations found magnitude R 2 * measurements. A significant, moderate negative correlation elastin fraction magnetic susceptibility, r −0.63 < 0.0001) found, as well a collagen −0.59 0.0001). Conclusion Tissue human shown be significantly correlated dominant of pathological cadaver samples—elastin collagen. Knowing that are disrupted vascular disease progression, offers clinically translatable potential novel biomarkers.

Language: Английский

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Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Oct. 20, 2022

Abstract Biological tissues comprise a spatially complex structure, composition and organization at the microscale, named microstructure. Given close structure-function relationships in tissues, structural characterization is essential to fully understand functioning of healthy pathological as well impact possible treatments. Here, we present nondestructive imaging approach perform quantitative 3D histo(patho)logy biological termed Cryogenic Contrast-Enhanced MicroCT (cryo-CECT). By combining sample staining, using an X-ray contrast-enhancing staining agent, with freezing optimal rate, cryo-CECT enables visualization analysis individual tissue constituents, such muscle collagen fibers. We applied on murine hearts subjected pressure overload following transverse aortic constriction surgery. Cryo-CECT allowed analyze, unprecedented manner, orientation diameter fibers entire heart, localization fibrotic regions within myocardial layers. foresee further applications optimization tissue/food preservation donor banking, showing that also has clinical industrial potential.

Language: Английский

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Multimodal experimental studies of the passive mechanical behavior of human aortas: Current approaches and future directions

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 178, P. 1 - 12

Published: Feb. 22, 2024

Cardiovascular diseases are the leading cause of death worldwide and include, among others, critical conditions aortic wall. Importantly, such require effective diagnosis treatment, which not yet accurate enough. However, they could be significantly strengthened with predictive material models In particular, support surgical decisions, preoperative planning, estimation postoperative tissue remodeling. developing a model requires experimental data showing both structural parameters mechanical behavior. Such can obtained using multimodal experiments. This review therefore discusses current approaches to strength wall is determined primarily by its passive components, i.e., mainly collagen, elastin, proteoglycans. Therefore, this focuses on experiments that relate behavior human structure organization components. classified according expected results. Multiple examples provided for each class summarized highlighted advantages disadvantages method. Finally, future directions envisioned evaluated. Multimodal innovative have gained interest very quickly, but also recently. presents first clear summary groundbreaking research in field The benefits limitations various types thoroughly discussed, comprehensive overview possible results provided. Although performed tissues, methods used described limited tissues extended other soft materials.

Language: Английский

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Phosphotungstic acid (PTA) preferentially binds to collagen- rich regions of porcine carotid arteries and human atherosclerotic plaques observed using contrast enhanced micro-computed tomography (CE-µCT)

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 2, 2023

Background and aims: Atherosclerotic plaque rupture in the carotid artery can cause small emboli to travel cerebral arteries, causing blockages preventing blood flow leading stroke. Contrast enhanced micro computed tomography (CEμCT) using a novel stain, phosphotungstic acid (PTA) provide insights into microstructure of vessel wall atherosclerotic plaque, hence their likelihood rupture. Furthermore, it has been suggested that collagen content orientation be related mechanical integrity. This study aims build on existing literature establish robust reproducible staining imaging technique non-destructively quantify within arteries plaques as an alternative routine histology. Methods: Porcine human were stained with concentration 1% PTA solution imaged MicroCT situ architecture tissue measure content. A histological assessment was also performed from picrosirius red (PSR) staining. Results: arterial samples highlight reproducibility used quantitative analysis showing positive correlation between measured CEμCT collagen-rich areas clearly visualised both plaque. 3D reconstruction different layers various components differentiated Hounsfield Unit (HU) values. Conclusion: The work presented here is unique offers method segmenting its individual quantifying these tissues, whilst delivering visual representation fibrous structure single contrast agent.

Language: Английский

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Non-destructive 3D characterization of the blood vessel wall microstructure in different species and blood vessel types using contrast-enhanced microCT and comparison with synthetic vascular grafts

Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 164, P. 303 - 316

Published: April 16, 2023

Language: Английский

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3-Dimensional Investigation of the Vascular Anatomy of the Triquetrum with Micro-Computed Tomography

Annals of Anatomy - Anatomischer Anzeiger, Journal Year: 2025, Volume and Issue: 259, P. 152377 - 152377

Published: Jan. 16, 2025

Language: Английский

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Beneath the Perforators: Unravelling Fascia Lata Intrinsic Vascular Architecture using microfocus X-ray computed tomography

Annals of Anatomy - Anatomischer Anzeiger, Journal Year: 2025, Volume and Issue: unknown, P. 152386 - 152386

Published: Feb. 1, 2025

Language: Английский

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A review of in-situ mechanical testing combined with X-ray microfocus computed tomography: application and current challenges for biological tissues.

Tomography of Materials and Structures, Journal Year: 2025, Volume and Issue: unknown, P. 100062 - 100062

Published: April 1, 2025

Language: Английский

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X-Ray-Based 3D Histo(patho)logy of Biological Tissues Using Contrast-Enhanced MicroCT

Reference series in biomedical engineering, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 43

Published: Jan. 1, 2025

Language: Английский

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