Mechanosensing by Piezo1 and its implications in the kidney

Acta Physiologica, Journal Year: 2024, Volume and Issue: 240(6)

Published: April 29, 2024

Abstract Piezo1 is an essential mechanosensitive transduction ion channel in mammals. Its unique structure makes it capable of converting mechanical cues into electrical and biological signals, modulating (patho)physiological processes a wide variety cells. There increasing evidence demonstrating that the piezo1 plays vital role renal physiology disease conditions. This review summarizes current on properties Piezo1, gating modulation, pharmacological characteristics, with special focus distribution significance kidney, which may provide insights potential treatment targets for diseases involving this channel.

Language: Английский

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Bone Health in Children with Rheumatic Disorders: Focus on Molecular Mechanisms, Diagnosis, and Management

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(10), P. 5725 - 5725

Published: May 20, 2022

Bone is an extremely dynamic and adaptive tissue, whose metabolism homeostasis influenced by many different hormonal, mechanical, nutritional, immunological pharmacological stimuli. Genetic factors significantly affect bone health, through their influence on cells function, cartilage quality, calcium vitamin D homeostasis, sex hormone pubertal timing. In addition, optimal nutrition physical activity contribute to mass acquisition in the growing age. All these attainment of peak mass, a critical determinant health fracture risk adulthood. Secondary osteoporosis important issue clinical care children with acute chronic diseases. Systemic autoimmune disorders, like juvenile idiopathic arthritis, can skeletal system, causing reduced mineral density high fragility fractures during childhood. patients, multiple reduce strength, including systemic inflammation elevated cytokines, activity, malabsorption nutritional deficiency, inadequate daily intake, use glucocorticoids, poor growth delay. more prominent at femoral neck radius compared lumbar spine. Nevertheless, vertebral are important, often asymptomatic manifestation, especially glucocorticoid-treated patients. A standardized diagnostic approach musculoskeletal prophylaxis, therapy follow up, therefore mandatory children. Here we discuss molecular mechanisms involved disease metabolism.

Language: Английский

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The Impact of Plasma Membrane Ion Channels on Bone Remodeling in Response to Mechanical Stress, Oxidative Imbalance, and Acidosis

Antioxidants, Journal Year: 2023, Volume and Issue: 12(3), P. 689 - 689

Published: March 10, 2023

The extracellular milieu is a rich source of different stimuli and stressors. Some them depend on the chemical-physical features matrix, while others may come from 'outer' environment, as in case mechanical loading applied bones. In addition to these forces, plethora chemical signals drives cell physiology fate, possibly leading dysfunctions when homeostasis disrupted. This variety triggers responses among tissues: bones represent particular which fragile balance between metabolic demands should be tuned maintained by concerted activity biomolecules located at interface external internal environments. Plasma membrane ion channels can viewed multifunctional protein machines that act rapid selective dual-nature hubs, sensors, transducers. Here we focus some multisensory (belonging Piezo, TRP, ASIC/EnaC, P2XR, Connexin, Pannexin families) actually or potentially playing significant role bone adaptation three main stressors, oxidative stress, acidosis, through their effects cells including mesenchymal stem cells, osteoblasts, osteoclasts, osteocytes. Ion channel-mediated remodeling occurs physiological processes, aging, human diseases such osteoporosis, cancer, traumatic events.

Language: Английский

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Piezo1 activation increases the release of therapeutic extracellular vesicles after mechanical stimulation in bioreactors

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Abstract Enhancing protocols and methods for producing therapeutic extracellular vesicles (EVs) in bioreactors is crucial to achieve scalable production while ensuring both quality quantity. Studies have shown that mechanical stress can promote EV release, although the underlying mechanisms remain largely unclear. Here we investigated which are responsible increase of under shear stress. EVs were produced from adipose tissue-derived stromal cells (ASCs), also described as mesenchymal (MSCs) support regeneration injured tissues via several paracrine factors. The cultures treated with GsMTx4, an inhibitor blocks Piezo1 ion channels, or YODA1, its agonist, assess if forces pathways involved enhancing release. quantified characterized after high (HS) stimulation compared no (3D) standard (2D). These experiments showed that, by stress, increased this effect was blocked inhibition channels GsMTx4 (88%) impact on cell viability. Consistently, agonist YODA1 (149%). implications these findings significant, especially regenerative medicine cellular therapies, where efficient high-quality crucial. By understanding turbulence-induced natural mechanotransductive within cells, it may be possible optimize EVs, paving way more effective treatments future.

Language: Английский

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Research progress on PIEZO1 protein structure and activation mechanism by small-molecule agonists

Results in Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 102058 - 102058

Published: Jan. 1, 2025

Language: Английский

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