Light‐Up Sensing Citrate Using a Capture‐Selected DNA Aptamer DOI
Lide Gu, Yao Zhang, Deli Wang

et al.

Advanced Sensor Research, Journal Year: 2024, Volume and Issue: 3(6)

Published: Feb. 22, 2024

Abstract Citrate is a key metabolite and nutrient in humans. Its level associated with many diseases from tumor growth to bone diseases. Detection of citrate has relied on its high negative charge, metal chelating properties as an enzyme substrate. In this work, the capture‐selection method used isolate DNA aptamers for citrate. After 18 rounds selection, highly converged library obtained first two sequences reached 99.6% library. Using most abundant sequence named CA1, thioflavin T fluorescence spectroscopy isothermal titration calorimetry show dissociation constants 7.4 4.4 µ m citrate, respectively. CA1 does not require sodium binding but requires 1.0 magnesium. Among tested carboxylate molecules, only can bind aptamer. A light‐up strand displacement biosensor developed it detect simulated urine detection limit 1.1 . This short 42‐nucleotide aptamer be readily adapted other types sensing mechanisms

Language: Английский

An Overview: The Diversified Role of Mitochondria in Cancer Metabolism DOI Creative Commons
Yue Liu,

Yihong Sun,

Yadong Guo

et al.

International Journal of Biological Sciences, Journal Year: 2023, Volume and Issue: 19(3), P. 897 - 915

Published: Jan. 1, 2023

Mitochondria are intracellular organelles involved in energy production, cell metabolism and signaling.They essential not only the process of ATP synthesis, lipid nucleic acid metabolism, but also tumor development metastasis.Mutations mtDNA commonly found cancer cells to promote rewiring bioenergetics biosynthesis, various metabolites especially oncometabolites mitochondria regulate progression.And mutation enzymes TCA cycle leads unusual accumulation certain oncometabolites.Mitochondria have been demonstrated as target for treatment.Cancer rely on two main resources: oxidative phosphorylation (OXPHOS) glycolysis.By manipulating OXPHOS genes or adjusting production mitochondria, growth can be restrained.For example, enhanced complex I activity increases NAD + /NADH prevent metastasis progression cancers.In this review, we discussed mitochondrial function specially explored unique role stem microenvironment.Targeting pathway mitochondria-related emerging a potential therapeutic strategy cancers.

Language: Английский

Citations

111
Role of AMPK in autophagy DOI Creative Commons
Shengyuan Wang, Hongyan Li, Minghao Yuan

et al.

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13

Published: Nov. 25, 2022

Adenosine monophosphate-activated protein kinase (AMPK) is a significant energy sensor in the maintenance of cellular homeostasis. Autophagy highly conserved catabolic process that involves an intracellular degradation system which cytoplasmic components, such as aggregates, organelles, and other macromolecules, are directed to lysosome through self-degradative maintain Given triggered autophagy various situations including nutrient deficit, AMPK potentially linked with different stages autophagy. Above all, increases ULK1 activity by directly phosphorylating Ser467, Ser555, Thr574, Ser637 at least four sites, recruitment autophagy-relevant proteins (ATG proteins) membrane domains affects initiation stage. Secondly, inhibits VPS34 complexes do not contain pro-autophagic factors thus involved isolation forming processes, direct phosphorylation on Thr163 Ser165. After phosphorylation, can govern autophagosome formation recruiting downstream autophagy-related site. Finally, AMPK-SIRT1 signaling pathway be activated upregulating transcription genes, thereby enhancing autophagosome-lysosome fusion. This review provides introduction role

Language: Английский

Citations

102
Acetyl-CoA regulates lipid metabolism and histone acetylation modification in cancer DOI
Weijing He, Qingguo Li, Xinxiang Li

et al.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2022, Volume and Issue: 1878(1), P. 188837 - 188837

Published: Nov. 17, 2022

Language: Английский

Citations

73
Multimetallic transition metal complexes:Luminescent probes for biomolecule sensing, ion detection, imaging and therapeutic application DOI
Bishnu Das, Parna Gupta

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 504, P. 215656 - 215656

Published: Jan. 19, 2024

Language: Английский

Citations

26
The strategic roles of four enzymes in the interconnection between metabolism and oncogene activation in non-small cell lung cancer: Therapeutic implications DOI Creative Commons
Philippe Icard, Luca Simula,

Ludovic Fournel

et al.

Drug Resistance Updates, Journal Year: 2022, Volume and Issue: 63, P. 100852 - 100852

Published: July 1, 2022

Language: Английский

Citations

40
Epigenetic modification of ferroptosis by non-coding RNAs in cancer drug resistance DOI Creative Commons
Hongquan Wang, Joshua S. Fleishman,

Sihang Cheng

et al.

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: Aug. 27, 2024

Abstract The development of drug resistance remains a major challenge in cancer treatment. Ferroptosis, unique type regulated cell death, plays pivotal role inhibiting tumour growth, presenting new opportunities treating chemotherapeutic resistance. Accumulating studies indicate that epigenetic modifications by non-coding RNAs (ncRNA) can determine vulnerability to ferroptosis. In this review, we first summarize the growth/development. Then, core molecular mechanisms ferroptosis, its upstream regulation, and downstream effects on Finally, review recent advances understanding how ncRNAs regulate ferroptosis from such modulate This aims enhance general ncRNA-mediated regulatory which highlighting ncRNA-ferroptosis axis as key druggable target overcoming

Language: Английский

Citations

15
Cancer immunometabolism: advent, challenges, and perspective DOI Creative Commons
Qin Dang, Borui Li,

Bing Jin

et al.

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: April 5, 2024

Abstract For decades, great strides have been made in the field of immunometabolism. A plethora evidence ranging from basic mechanisms to clinical transformation has gradually embarked on immunometabolism center stage innate and adaptive immunomodulation. Given this, we focus changes immunometabolism, a converging series biochemical events that alters immune cell function, propose roles played by diversified metabolic derivatives enzymes, emphasize key metabolism-related checkpoints distinct types, discuss ongoing upcoming realities treatment. It is expected future research will reduce current limitations immunotherapy provide positive hand responses exert broader therapeutic role.

Language: Английский

Citations

13
Biological and genetic determinants of glycolysis: Phosphofructokinase isoforms boost energy status of stored red blood cells and transfusion outcomes DOI
Travis Nemkov, Daniel Stephenson, Eric J. Earley

et al.

Cell Metabolism, Journal Year: 2024, Volume and Issue: 36(9), P. 1979 - 1997.e13

Published: July 3, 2024

Language: Английский

Citations

12
The Tricarboxylic Acid Cycle Metabolites for Cancer: Friend or Enemy DOI Creative Commons
Jie Wu, Nian Liu, Jing Chen

et al.

Research, Journal Year: 2024, Volume and Issue: 7

Published: Jan. 1, 2024

The tricarboxylic acid (TCA) cycle is capable of providing sufficient energy for the physiological activities under aerobic conditions. Although tumor metabolic reprogramming places glycolysis in a dominant position, TCA remains indispensable cells as hub linkage and interconversion glucose, lipids, certain amino acids. intermediates such citrate, α-ketoglutarate, succinate, fumarate are altered tumors, they regulate metabolism, signal transduction, immune environment to affect tumorigenesis progression. This article provides comprehensive review modifications occurring relation cycle, which affects pathogenesis current therapeutic strategy therapy through targeting cancer cells.

Language: Английский

Citations

9
Terahertz Metamaterial Sensor for Sensitive Detection of Citrate Salt Solutions DOI Creative Commons
Xinxin Deng,

Yanchun Shen,

Bingwei Liu

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 12(6), P. 408 - 408

Published: June 13, 2022

Citrate salts (CSs), as one type of organic salts, have been widely used in the food and pharmaceutical industries. Accurate quantitative detection CSs medicine is very important for health safety. In this study, an asymmetric double-opening ring metamaterial sensor designed, fabricated, to detect citrate combined with THz spectroscopy. Factors that influence sensitivity including opening positions arrangement metal unit, refraction index thickness analyte deposited on were analyzed discussed from electromagnetic simulations spectroscopy measurements. Based high refractive analyte, six different salt solutions low concentrations well identified. Therefore, a metamaterials can provide new, rapid, accurate salts.

Language: Английский

Citations

36