Potential Value of Expression of Receptor Accessory Protein 4 in  Lower - Grade Glioma For Antitumor Immunotherapy DOI
Zhendong Liu, Haigang Chang, Xingbo Cheng

et al.

SSRN Electronic Journal, Journal Year: 2022, Volume and Issue: unknown

Published: Jan. 1, 2022

Background: Mitosis plays an important role in tumor cell proliferation and REEP4 is involved the regulation of biological process mitosis; however, it has not been reported malignant tumors to date.Methods: We collected transcriptome data, DNA methylation data clinical characteristics thousands patients with lower-grade glioma (LGG). Various big analysis methods molecular biology experiments were employed reveal impact on pathological LGG. Findings: It was found that expression significantly elevated negatively regulated by its site. Therefore, both high low state cg16311504 showed exhibited reduced survival time. In addition, promotes various cancer-related cellular signaling pathways, such as cycle, MAPK pathway, NOD-like receptor etc. More importantly, a significant increase infiltration level immune cells LGG microenvironment positive correlation PD-L1 other checkpoints. Interpretation: brief, this study first introduce tumors, which can be used independent pathogenic gene participates potential become new star field anti-tumor immunotherapy.Funding Information: This work supported Tackling key problems science technology Henan Province (Grant ID: 222102310002) The Medical Science research plan LHGJ20210013). Declaration Interests: No conflicts interest disclosed.Ethics Approval Statement: All tissue samples obtained from operating room Provincial People's Hospital placed into -80 refrigerator for storage until use. collection approved ethics committee (2020107).

Language: Английский

The Involvement of Long Non-Coding RNAs in Glutamine-Metabolic Reprogramming and Therapeutic Resistance in Cancer DOI Open Access
Jungwook Roh,

Mijung Im,

Yeonsoo Chae

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(23), P. 14808 - 14808

Published: Nov. 26, 2022

Metabolic alterations that support the supply of biosynthetic molecules necessary for rapid and sustained proliferation are characteristic cancer. Some cancer cells rely on glutamine to maintain their energy requirements growth. Glutamine is an important metabolite in because it not only links tricarboxylic acid cycle by producing α-ketoglutarate glutaminase glutamate dehydrogenase but also supplies other non-essential amino acids, fatty components nucleotide synthesis. Altered metabolism associated with cell survival, proliferation, metastasis, aggression. Furthermore, altered known be involved therapeutic resistance. In recent studies, lncRNAs were shown act transporters glutamine-metabolic enzymes, resulting regulation metabolism. The expression include abhydrolase domain containing 11 antisense RNA 1, LINC00857, plasmacytoma variant translocation Myc-induced long non-coding RNA, opa interacting protein 5 all which play oncogenic roles. When comes several lncRNAs, including nuclear paraspeckle assembly transcript XLOC_006390, urothelial thymopoietin show activities, others such as lncRNA glutaminase, lincRNA-p21, ataxin 8 opposite strand serve tumor suppressors. addition, glutamine-dependent dysregulation promote metastasis increasing chemo- radio-resistance. Therefore, understanding roles will helpful establishment strategies patients.

Language: Английский

Citations

11
Exosomal long non-coding RNAs in glioblastoma DOI
Mehdi Fattahi,

Vahab Alamdari-Palangi,

Khojaste Rahimi Jaberi

et al.

Clinica Chimica Acta, Journal Year: 2023, Volume and Issue: 553, P. 117705 - 117705

Published: Dec. 10, 2023

Language: Английский

Citations

6
HOTTIP Mediated Therapy Resistance in Glioma Cells Involves Regulation of EMT-Related miR-10b DOI Creative Commons
Zhang Li, Ming Li, Pengcheng Xia

et al.

Frontiers in Oncology, Journal Year: 2022, Volume and Issue: 12

Published: March 24, 2022

The advanced grade glioblastomas are characterized by dismal five-year survival rates and associated with worse outcomes. Additionally, resistance to therapies is an additional burden responsible for glioma mortality. We studied the against temozolomide (TMZ) as a surrogate understand mechanism of therapy in cancer cells. Screening three cells lines, A172, LN229 SF268 revealed that were particularly resistant TMZ IC-50 this cell line ten times higher than other two lines. A role lncRNAs progression has been identified recent years and, therefore, we focused on their regulating lncRNA HOTTIP was found be elevated over-expression both A172 remarkably increased IC-50s, along proliferation, migration, clonogenicity markers angiogenesis metastasis. As observed expression miRNA-10b mesenchymal Zeb1/Zeb2 reduced E-cadherin indicating EMT resistance. overexpressed also had similarly induced miR-10b levels. Further, silencing overexpressing well reversed sensitization all tested TMZ. Our results thus present case native acquired chemotherapy, key mechanistic miR-10b. Thus, critical targets therapy, need further.

Language: Английский

Citations

9
LINC02774 inhibits glycolysis in glioma to destabilize HIF‐1α dependent on transcription factor RP58 DOI Creative Commons

Yuanbing Chen,

Yating Liu, Jian‐Bing Xiong

et al.

MedComm, Journal Year: 2023, Volume and Issue: 4(5)

Published: Sept. 11, 2023

Glioma, the most common of malignant tumors in brain, is responsible for majority deaths from primary brain tumors. The regulation long noncoding RNAs (lncRNAs) HIF-1α-driven tumor development remains unclear. LINC02774 a nuclear lncRNA and that it being reported first time this study. We found downregulation glioma decreased with degree malignant, its expression showing negative correlation relative index enhanced magnetic resonance (RIEMR). RIEMR-associated was to inhibit glycolysis by modulating hypoxia pathway rather than response itself. interacted neighboring gene, RP58 (ZBTB18), enhance PHD3, which catalyzed HIF-1α hydroxylase ubiquitination, leading expression. also function LINC02774, dependent on diminished upon depletion. Notably, higher associated favorable prognosis. In conclusion, these findings reveal role relies neighbor RP58, regulate as novel suppressor, suggesting potential be prognostic marker molecular target therapy glioma.

Language: Английский

Citations

4
Ferroptosis-related lncRNAs: Distinguishing heterogeneity of the tumour microenvironment and predicting immunotherapy response in bladder cancer DOI Creative Commons
Zhan Yang, Xiaoqi Li,

Lijun Zhou

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(11), P. e32018 - e32018

Published: May 31, 2024

Ferroptosis, a cell death pathway dependent on iron, has been shown in research to play role the development, advancement, and outlook of tumours through ferroptosis-related lncRNAs (FRLRs). However, value FRLRs bladder cancer (BLCA) not thoroughly investigated. This project involved developing predictive model using ten specific (AC099850.4, AL731567.1, AL133415.1, AC021321.1, SPAG5-AS1, HMGA2-AS1, RBMS3-AS3, AC006160.1, AL583785.1, AL662844.4) univariate COX LASSO regression techniques. The validation this signature as standalone predictor was confirmed group 65 patients from urology tumour database at First Affiliated Hospital Wenzhou Medical University Wenzhou, China. Patients were categorized based their median risk score into either low-risk or high-risk group. Enrichment analysis identified possible molecular mechanisms that could explain variations clinical outcomes observed groups. Moreover, we explored correlation between FLPS immunotherapy-related indicators. ability forecast effectiveness immunotherapy validated by elevated levels immune checkpoint genes (PD-L1, CTLA4, PD-1) high risk. We also screened crucial FRLR (HMGA2-AS1) congruent expression prognostic conditions established ceRNA network, indicating HMGA2-AS1 may affect epithelial-mesenchymal transition modulating Wnt signalling mechanism. top five mRNAs (NFIB, NEGR1, JAZF1, JCAD, ESM1) random forest algorithm analysed relationship mRNAs, immunotherapy, interactions with drug sensitivities. Our results suggest BLCA have greater sensitivity four drugs (dasatinib, pazopanib, erismodegib olaparib). study provides new insights TME, key pathways, genome, potential therapeutic targets BLCA, future guidance for targeted precision drugs.

Language: Английский

Citations

1
CREB-induced LINC00473 promotes chemoresistance to TMZ in glioblastoma by regulating O6-methylguanine-DNA-methyltransferase expression via CEBPα binding DOI Creative Commons

Liya Jiang,

Guan-Hao Wang,

Jing-Jiao Xu

et al.

Neuropharmacology, Journal Year: 2023, Volume and Issue: 243, P. 109790 - 109790

Published: Nov. 21, 2023

Language: Английский

Citations

3
Hippo-YAP/TAZ pathway regulation: the crucial roles of lncRNAs in cancer DOI Creative Commons

Wonyi Jang,

Mijung Im,

Jungwook Roh

et al.

Animal Cells and Systems, Journal Year: 2023, Volume and Issue: 27(1), P. 309 - 320

Published: Nov. 21, 2023

The Hippo signaling pathway is a conserved that regulates cell proliferation, apoptosis, and differentiation plays essential roles in the maintenance of organ size tissue homeostasis. Also, this critical regulator malignant progression wide range cancers. Furthermore, it has been established YAP TAZ are major effectors transcription co-activators regulate expressions genes involved growth differentiation. Long non-coding RNAs (lncRNAs) transcripts longer than 200 nucleotides several biological processes, including chromatin remodeling, transcriptional regulation, post-transcriptional processing. Evidence suggests aberrant expression dysregulation lncRNAs contribute to development various malignancies by acting as oncogenes or tumor suppressors. In addition, many studies have shown can directly indirectly Hippo-YAP/TAZ cancer. Here, we overview played with focus on their regulation discuss potential usages clinical biomarkers therapeutic targets.

Language: Английский

Citations

3
LncRNA PANTR1 is Associated with Poor Prognostic and Suppresses Apoptosis in Glioma DOI Creative Commons
Fei Shi, Jie Hu, Ping Zheng

et al.

Journal of Oncology, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 14

Published: Feb. 20, 2023

Glioma is the most common tumor in central nervous system. High-grade gliomas confer a poor prognosis, being serious health and economic burden. Current literature suggests important role of long noncoding RNA (lncRNA) mammals, especially tumorigenesis various tumors. The functions lncRNA POU3F3 adjacent transcript 1 (PANTR1) have been investigated hepatocellular carcinoma but remain yet unclear gliomas. We evaluated PANTR1 glioma cells using published data from Cancer Genome Atlas (TCGA), then validated it by ex vivo experiments. To investigate potential cellular mechanism different levels expression cells, we used siRNA-mediated knockdown low-grade (grade II) cell lines GBM IV) (SW1088 SHG44, respectively). On molecular level, low caused significantly reduced viability enhanced death. Moreover, identified importance for migration both lines, critical foundation invasiveness recurrent In conclusion, this study provides first evidence that has relevant human influencing

Language: Английский

Citations

2
Potential value of expression of receptor accessory protein 4 for evaluating the prognosis of lower-grade glioma patients DOI Creative Commons

Shuping Luo,

Zhendong Liu, Haigang Chang

et al.

Aging, Journal Year: 2024, Volume and Issue: unknown

Published: March 28, 2024

Background: REEP4 is involved in the regulation of biological process mitosis. Lower grade glioma (LGG), as a malignant tumor, accompanied by abnormalities mitosis, but there have been no reports so far. Methods: We collected transcriptome data, DNA methylation data and clinical characteristics thousands patients with LGG. Various big analysis methods molecular biology experiments were employed to reveal impact on pathological Results: It was found that expression significantly elevated negatively regulated its site. Therefore, both high low state cg16311504 showed are correlated lower patient survival rate. In addition, participates various cancer-related cellular signaling pathways, such cell cycle, MAPK pathway, NOD-like receptor etc. More importantly, level immune infiltration increased group LGG tumor microenvironment has positive correlation PD-L1 other checkpoints. Conclusions: brief, this study first introduce tumors, which can be used an independent risk factor potential become new star field anti-tumor treatment.

Language: Английский

Citations

0
Gene Expression Profiling Regulated by lncRNA H19 Using Bioinformatic Analyses in Glioma Cell Lines DOI Open Access

Y.S. Chae,

Jungwook Roh, Myung Im

et al.

Cancer Genomics & Proteomics, Journal Year: 2024, Volume and Issue: 21(6), P. 608 - 621

Published: Oct. 28, 2024

Background/Aim: Glioma, the most common type of primary brain tumor, is characterized by high malignancy, recurrence, and mortality. Long non-coding RNA (lncRNA) H19 a potential biomarker for glioma diagnosis treatment due to its overexpression in human tissues involvement cell division metastasis regulation. This study aimed identify therapeutic targets involved development analyzing gene expression profiles regulated H19. Materials Methods: To elucidate role A172 U87MG lines, counting, colony formation, wound healing assays were conducted. RNA-seq data analysis bioinformatics analyses performed reveal molecular interactions Results: Cell-based experiments showed that elevated levels related cancer survival, proliferation, migration. Bioinformatics identified 2,084 differentially expressed genes (DEGs) influenced which are progression. Specifically, ANXA5, CLEC18B, RAB42, CXCL8, OASL, USP18, CDCP1 positively correlated with expression, while CSDC2 FOXO4 negatively correlated. These DEGs predicted function as oncogenes or tumor suppressors gliomas, association Conclusion: findings highlight associated diagnostic emphasizing their clinical significance patient survival.

Language: Английский

Citations

0