Cancer-associated fibroblasts drive colorectal cancer cell progression through exosomal miR-20a-5p-mediated targeting of PTEN and stimulating interleukin-6 production DOI Creative Commons

Mahsa Ghofrani-Shahpar,

Katayoon Pakravan,

Ehsan Razmara

et al.

BMC Cancer, Journal Year: 2024, Volume and Issue: 24(1)

Published: April 1, 2024

Abstract Background This study evaluated the clinical relevance of a set five serum-derived circulating microRNAs (miRNAs) in colorectal cancer (CRC). Additionally, we investigated role miR-20a-5p released by exosomes derived from cancer-associated fibroblasts (CAFs) context CRC. Methods The expression levels miRNAs (miR-20a-5p, miR-122-5p, miR-139-3p, miR-143-5p, and miR-193a-5p) were quantified real-time quantitative PCR (RT-qPCR), their associations with clinicopathological characteristics CRC patients assessed. diagnostic accuracy these was determined through Receiver Operating Characteristic (ROC) curve analysis. CAFs normal (NFs) isolated tissue samples, subsequently, cells meticulously characterized using electron microscopy Western blotting. cellular internalization fluorescent-labeled visualized confocal microscopy. Gain- loss-of-function experiments conducted to elucidate oncogenic transferred progression. underlying mechanisms uncovered luciferase reporter assay, blotting, enzyme-linked immunosorbent assays, as well proliferation migration assays. Results miR-122-5p significantly higher positively correlated advanced stages tumorigenesis lymph node metastasis (LNM). In contrast, miR-193a-5p down-regulated associated early tumorigenesis. Except for they showed negative correlation LNM status. Among candidate miRNAs, elevated observed both exosomal fractions CAFs. Our findings indicated that induces EMT markers, partly targeting PTEN. Exosomal miR-20a secreted emerged key factor enhancing cells. inhibition impaired proliferative migratory potential CAF-derived SW480 cells, suggesting effects are mediated transfer miR-20a. Furthermore , originating induced increased nuclear translocation NF-kB p65 transcription leading interleukin-6 (IL-6) production. Conclusions We established non-invasive biomarker diagnosis. our shed light on intricate underpinning impacts underscore pivotal

Language: Английский

Tumor Vaccines: Unleashing the Power of the Immune System to Fight Cancer DOI Creative Commons
Dequan Liu, Xiangyu Che, Xiaoxi Wang

et al.

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(10), P. 1384 - 1384

Published: Sept. 29, 2023

This comprehensive review delves into the rapidly evolving arena of cancer vaccines. Initially, we examine intricate constitution tumor microenvironment (TME), a dynamic factor that significantly influences heterogeneity. Current research trends focusing on harnessing TME for effective vaccine treatments are also discussed. We then provide detailed overview current state concerning immunity and mechanisms vaccines, describing complex immunological processes involved. Furthermore, conduct an exhaustive analysis contemporary landscape with particular focus peptide DNA/RNA-based viral-vector-based dendritic-cell-based whole-cell-based analyze summarize these categories highlighting their individual advantages, limitations, factors influencing effectiveness. In our survey each category, commonly used aiming to readers more understanding research. delve innovative strategy combining vaccines other therapies. By studying effects immune checkpoint inhibitors, radiotherapy, chemotherapy, targeted therapy, oncolytic virotherapy, establish this approach can enhance overall treatment efficacy offset limitations single-treatment approaches, offering patients options. Following this, undertake meticulous entire process personalized elucidating from design, through production, clinical application, thus helping gain thorough its complexities. conclusion, exploration in aims highlight promising potential treatment. As field continues evolve, it undeniably holds immense promise improving patient outcomes.

Language: Английский

Citations

34
Current Understanding of Microbiomes in Cancer Metastasis DOI Open Access
Jiaqi Liu,

Feiyang Luo,

Liyan Wen

et al.

Cancers, Journal Year: 2023, Volume and Issue: 15(6), P. 1893 - 1893

Published: March 22, 2023

Cancer has been the first killer that threatens people’s lives and health. Despite recent improvements in cancer treatment, metastasis continues to be main reason for death from cancer. The functions of microbiome have studied recently, it is proved can influence tumor metastasis, as well positive or negative responses therapy. Here, we summarize mechanisms microorganisms affecting which include epithelial-mesenchymal transition (EMT), immunity, fluid shear stress (FSS), matrix metalloproteinases (MMPs). This review will not only give a further understanding relationship between but also provide new perspective microbiome’s application prevention, early detection, treatment.

Language: Английский

Citations

24
Crosstalk between T lymphocyte and extracellular matrix in tumor microenvironment DOI Creative Commons

Die Lv,

Yujie Fei,

Hongli Chen

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: April 16, 2024

The extracellular matrix (ECM) is a complex three-dimensional structure composed of proteins, glycans, and proteoglycans, constituting critical component the tumor microenvironment. Complex interactions among immune cells, matrix, cells promote development metastasis, consequently influencing therapeutic efficacy. Hence, elucidating these interaction mechanisms pivotal for precision cancer therapy. T lymphocytes are an important system, exerting direct anti-tumor effects by attacking or releasing lymphokines to enhance effects. ECM significantly influences function infiltration within microenvironment, thereby impacting behavior biological characteristics cells. involved in regulating synthesis, degradation, remodeling through secretion cytokines enzymes. As result, it affects proliferation invasive ability as well efficacy immunotherapy. This review discusses underlying lymphocyte-ECM microenvironment their potential application It provides novel insights innovative strategies drug.

Language: Английский

Citations

11
Chitosan: modification and biodegradability of by-products DOI
Great Iruoghene Edo, Emad͏͏͏͏͏͏͏͏͏͏͏͏͏͏͏͏͏͏͏ Yousif, Mohammed H. Al-Mashhadani

et al.

Polymer Bulletin, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Language: Английский

Citations

10
Potential of MMP-2 and MMP-9 Gelatinase Blockade as a Therapeutic Strategy in Fibrosarcoma Treatment: A Decadal Review DOI Open Access
Alireza Shoari

Targets, Journal Year: 2024, Volume and Issue: 2(2), P. 104 - 125

Published: June 5, 2024

Fibrosarcoma represents a significant challenge in oncology, characterized by high invasiveness and poor prognosis. Gelatinases, particularly matrix metalloproteinases MMP-2 MMP-9, play pivotal role the degradation of extracellular matrix, facilitating tumor invasion metastasis. Inhibiting these enzymes has emerged as promising therapeutic strategy. This review evaluates progress development potential gelatinase inhibitors treatments for fibrosarcoma over last decade, highlighting molecular mechanisms future directions. A comprehensive literature was conducted, focusing on studies published from 2013 to 2023. Research articles papers relevant inhibition were examined assess efficacy inhibitors. Gelatinase have shown reduce progression, invasion, metastasis fibrosarcoma. Clinical trials, although limited, indicated that can be effectively integrated into existing regimens, offering reduction metastatic spread potentially improving patient survival rates. Mechanistic suggest MMP-9 disrupts critical pathways involved growth cell invasion. viable approach treatment. Future research should focus developing more specific inhibitors, understanding long-term outcomes, integrating multimodal treatment strategies enhance efficacy.

Language: Английский

Citations

9
A stumbling block in pancreatic cancer treatment: drug resistance signaling networks DOI Creative Commons
Jinming Liu, Biao Zhang,

Bingqian Huang

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 13, 2025

The primary node molecules in the cell signaling network cancer tissues are maladjusted and mutated comparison to normal tissues, which promotes occurrence progression of cancer. Pancreatic (PC) is a highly fatal with increasing incidence low five-year survival rates. Currently, there several therapies that target networks PC. However, PC "cold tumor" unique immunosuppressive tumor microenvironment (poor effector T infiltration, antigen specificity), targeting single gene or pathway basically ineffective clinical practice. Targeted matrix therapy, targeted metabolic mutant vaccines, other emerging have shown great therapeutic potential, but results been disappointing. Therefore, we summarize identified potential drug-resistant aimed at overcoming barriers existing therapies.

Language: Английский

Citations

1
Tunable membrane penetration-directed signal amplification for electrochemical determination of matrix metalloproteinase-2 in cancer diagnosis DOI

Guozhang Zhou,

Yizhang Wu,

Yulin Xiong

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 431, P. 137410 - 137410

Published: Feb. 7, 2025

Language: Английский

Citations

1
Long non-coding RNAs: Emerging regulators of invasion and metastasis in pancreatic cancer DOI Creative Commons
Mengmeng Shi, Rui Zhang, Hui Lyu

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

The invasion and metastasis of pancreatic cancer (PC) are key factors contributing to disease progression poor prognosis. This process is primarily driven by EMT, which has been the focus recent studies highlighting role long non-coding RNAs (lncRNAs) as crucial regulators EMT. However, mechanisms lncRNAs influence invasive multifaceted, extending beyond EMT regulation alone. review aims characterize affecting in cancer. We summarize regulatory roles across multiple molecular pathways highlight their translational potential, considering implications for clinical applications diagnostics therapeutics. focuses on three principal scientific themes. First, we orchestrate various signaling pathways, such TGF-β/Smad, Wnt/β-catenin, Notch, regulate changes associated with thereby enhancing cellular motility invasivenes. Second, effects autophagy ferroptosis discuss exosomal tumor microenvironment behavior neighboring cells promote cell invasion. Third, emphasize RNA modifications (such m6A m5C methylation) stabilizing capacity mediate PC. Lastly, potential these findings, emphasizing inherent challenges using biomarkers therapeutic targets, while proposing prospective research strategies.

Language: Английский

Citations

1
The Impact of Obesity, Adipose Tissue, and Tumor Microenvironment on Macrophage Polarization and Metastasis DOI Creative Commons
Ola Habanjar,

Mona Diab‐Assaf,

Florence Caldefie-Chézet

et al.

Biology, Journal Year: 2022, Volume and Issue: 11(2), P. 339 - 339

Published: Feb. 21, 2022

Tumor metastasis is a major cause of death in cancer patients. It involves not only the intrinsic alterations within tumor cells, but also crosstalk between these cells and components microenvironment (TME). Tumorigenesis complex dynamic process, involving following three main stages: initiation, progression, metastasis. The transition stages depends on changes extracellular matrix (ECM), which stromal reside. This matrix, under effect growth factors, cytokines, adipokines, can be morphologically altered, degraded, or reorganized. Many cancers evolve to form an immunosuppressive TME locally create pre-metastatic niche other tissue sites. niches include myofibroblasts, immuno-inflammatory (macrophages), adipocytes, blood, lymphatic vascular networks. Several studies have highlighted adipocyte-macrophage interaction as key driver progression dissemination. two classes macrophages are distinguished: M1 (pro-inflammatory/anti-tumor) M2 (anti-inflammatory/pro-tumor). These exhibit distinct microenvironment-dependent phenotypes that promote inhibit On hand, obesity patients has been linked poor prognosis. In this regard, tumor-associated adipocytes modulate through secretion inflammatory mediators, recruit (TAM). Hereby, review describes cellular molecular mechanisms link inflammation, obesity, cancer. provides comprehensive overview ECM they control invasion. addition, it addresses anchoring recruitment for M1, M2, TAM macrophages, specifically highlighting their origin, classification, polarization, regulatory networks, well roles regulation angiogenesis, invasion, metastasis, immunosuppression, role process.

Language: Английский

Citations

29
Multi-Faceted Role of Luteolin in Cancer Metastasis: EMT, Angiogenesis, ECM Degradation and Apoptosis DOI Open Access
Maria Teresa Rocchetti, Francesco Bellanti, Mariia Zadorozhna

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(10), P. 8824 - 8824

Published: May 16, 2023

Luteolin (3',4',5,7-tetrahydroxyflavone), a member of the flavonoid family derived from plants and fruits, shows wide range biomedical applications. In fact, due to its anti-inflammatory, antioxidant immunomodulatory activities, Asian medicine has been using luteolin for centuries treat several human diseases, including arthritis, rheumatism, hypertension, neurodegenerative disorders various infections. Of note, displays many anti-cancer/anti-metastatic properties. Thus, purpose this review consists in highlighting relevant mechanisms by which inhibits tumor progression metastasis, i.e., affecting epithelial-mesenchymal transition (EMT), repressing angiogenesis lysis extracellular matrix (ECM), as well inducing apoptosis.

Language: Английский

Citations

22