Role of soluble epoxide hydrolase in the abnormal activation of fibroblast-like synoviocytes from patients with rheumatoid arthritis DOI Creative Commons
Yaoyu Pu,

Ruijuan Cheng,

Qiuping Zhang

et al.

Clinical Immunology, Journal Year: 2023, Volume and Issue: 257, P. 109850 - 109850

Published: Nov. 25, 2023

Rheumatoid arthritis (RA) is an autoimmune disease characterized by enigmatic pathogenesis. Polyunsaturated fatty acids (PUFAs) are implicated in RA's development and progression, yet their exact mechanisms of influence not fully understood. Soluble epoxide hydrolase (sEH) enzyme that metabolizes anti-inflammatory epoxy (EpFAs), derivatives PUFAs. In this study, we report elevated sEH expression the joints CIA (collagen-induced arthritis) rats, concomitant with diminished levels two significant EpFAs. Additionally, increased was detected both synovium rats fibroblast-like synoviocytes (FLS) RA patients. The inhibitor TPPU attenuated migration invasion capabilities FLS derived from patients to reduce secretion inflammatory factors these cells. Our findings indicate a pivotal role for pathogenesis suggest inhibitors offer promising new therapeutic strategy managing RA.

Language: Английский

A role of gut–microbiota–brain axis via subdiaphragmatic vagus nerve in depression-like phenotypes in Chrna7 knock-out mice DOI Creative Commons
Yong Yang, Akifumi Eguchi, Xiayun Wan

et al.

Progress in Neuro-Psychopharmacology and Biological Psychiatry, Journal Year: 2022, Volume and Issue: 120, P. 110652 - 110652

Published: Sept. 30, 2022

Language: Английский

Citations

40
The vagus nerve: An old but new player in brain–body communication DOI Creative Commons
Li Ma,

Hanbing Wang,

Kenji Hashimoto

et al.

Brain Behavior and Immunity, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

9
Synergistic neuroprotective effects of two natural medicinal plants against CORT-induced nerve cell injury by correcting neurotransmitter deficits and inflammation imbalance DOI
Jin Pan, Yanting Lu, Sijia Wang

et al.

Phytomedicine, Journal Year: 2023, Volume and Issue: 121, P. 155102 - 155102

Published: Sept. 18, 2023

Language: Английский

Citations

12
Lipid mediators generated by the cytochrome P450—Epoxide hydrolase pathway DOI
Timo Frömel, Jiong Hu, Ingrid Fleming

et al.

Advances in pharmacology, Journal Year: 2023, Volume and Issue: unknown, P. 327 - 373

Published: Jan. 1, 2023

Language: Английский

Citations

11
A Naturalistic Prospective Study of the Prognostic Impact of EPHX2 in Major Depressive Disorder: Impulsivity may be an Important Factor DOI Creative Commons
Shuqiong Zheng, Jia Guo, Rongxin Zheng

et al.

Depression and Anxiety, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Background: Major depressive disorder (MDD) is a leading cause of disability worldwide. The pathophysiology MDD remains unclear, which limits the development treatments for MDD. Recently, epoxide hydrolase 2 (EPHX2) has been found to be associated with Our previous study revealed an association between EPHX2 expression and suicide. However, effect on prognosis suicide unclear. Previous studies have that impulsivity at baseline can significant predictor clinical improvement in patients Therefore, we inferred could treatment MDD, mediate Methods: This naturalistic prospective included 117 participants who were assessed, using questionnaires, cognitive function, information, baseline, weeks, 1, 2, 3 months. A linear mixed‐effects model was used investigate longitudinal changes severity symptoms, risk suicide, function. Results: interactive effects polymorphisms (measured by Columbia‐Suicide Severity Rating Scale) significantly different rs11288636, rs68012435, rs11288636. time depression Hamilton Depression Scale‐24) including after adjustment total score. Conclusions: suggests are critical factor change among genotypes. Trial Registration: ClinicalTrials.gov identifier: NCT05575713

Language: Английский

Citations

0
Identification and characterization of the in-vivo metabolites of the novel soluble epoxide hydrolase inhibitor EC5026 using liquid chromatography quadrupole time of flight mass spectrometry DOI

Shankha Dey,

Rushikesh Biradar,

Sayalee Sanjay Mane

et al.

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2024, Volume and Issue: 244, P. 116116 - 116116

Published: March 22, 2024

Language: Английский

Citations

3
The involvement of soluble epoxide hydrolase in the development of cardiovascular diseases through epoxyeicosatrienoic acids DOI Creative Commons
Shan Jiang, Siyi Han, Dao Wen Wang

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: April 2, 2024

Arachidonic acid (AA) has three main metabolic pathways: the cycloxygenases (COXs) pathway, lipoxygenases (LOXs) and cytochrome P450s (CYPs) pathway. AA produces epoxyeicosatrienoic acids (EETs) through CYPs EETs are very unstable in vivo can be degraded seconds to minutes. have multiple degradation pathways, but mainly presence of soluble epoxide hydrolase (sEH). sEH is an enzyme bifunctional nature, current research focuses on activity its C-terminal (sEH-H), which hydrolyzes corresponding inactive or low diol. Previous studies reported that cardiovascular protective effects, sEH-H plays a role by degrading inhibiting their effects. The different cells, such as endothelial cell proliferation migration, promoting vascular smooth muscle migration. Therefore, it interest whether involved initiation progression diseases affecting function cells EETs.

Language: Английский

Citations

3
Inhibition of Soluble Epoxide Hydrolase by Cembranoid Diterpenes from Soft Coral Sinularia maxima: Enzyme Kinetics, Molecular Docking, and Molecular Dynamics DOI Creative Commons
Nguyen Viet Phong, Nguyễn Phương Thảo, Le Ba Vinh

et al.

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(8), P. 373 - 373

Published: Aug. 17, 2024

Soluble epoxide hydrolase (sEH) is essential for converting epoxy fatty acids, such as epoxyeicosatrienoic acids (EETs), into their dihydroxy forms. EETs play a crucial role in regulating blood pressure, mediating anti-inflammatory responses, and modulating pain, making sEH key target therapeutic interventions. Current research increasingly focused on identifying inhibitors from natural sources, particularly marine environments, which are rich bioactive compounds due to unique metabolic adaptations. In this study, the inhibitory activities of ten cembranoid diterpenes (1–10) isolated soft coral Sinularia maxima were evaluated. Among them, 3 9 exhibited considerable inhibition, with IC50 values 70.68 μM 78.83 μM, respectively. Enzyme kinetics analysis revealed that these two active inhibit through non-competitive mode. Additionally, silico approaches, including molecular docking dynamics simulations, confirmed stability interactions sEH, highlighting potential agents managing cardiovascular inflammatory diseases.

Language: Английский

Citations

3
n‐3 polyunsaturated fatty acids improve depression‐like behavior by inhibiting hippocampal neuroinflammation in mice via reducing TLR4 expression DOI Creative Commons
Li Hu,

Xianxiang Zeng,

Kai Yang

et al.

Immunity Inflammation and Disease, Journal Year: 2022, Volume and Issue: 10(11)

Published: Oct. 11, 2022

Abstract Introduction n‐3 polyunsaturated fatty acids (PUFAs) are believed to be implicated in the pathogenesis of many inflammation‐related diseases, including depression. Methods The mouse model depression was established through chronic unpredictable mild stress (CUMS), mice were intervened with PUFAs, and then expression toll‐like receptor 4 (TLR4) stimulated lipopolysaccharides (LPS). Tail suspension test (TST), forced swimming (FST) sucrose preference performed monitor behavior mice. Microglia activation detected by Iba1 immunofluorescence, neuronal injury Nissl staining. Concentrations tumor necrosis factor (TNF)‐α, Interleukin (IL)‐6 IL‐1β hippocampus assessed via enzyme linked immunosorbent assay (ELISA). Quantitative real time polymerase chain reaction used detect IL‐6, TNF‐α messenger RNA levels. Western blot utilized for detection TLR4 protein expression. Results CUMS significantly reduced mice, while increased immobility FST TST. Moreover, aggravated microglia damage levels hippocampal tissues, however, intervention PUFAs could improve above effects. Further, induced LPS partially reversed inhibition on depression‐like behaviors, microglial inflammatory neurons. Conclusion may ameliorate behaviors reducing neuroinflammation CUMS‐induced regulating expression, suggesting that an effective antidepressant, which provides evidence future treatment

Language: Английский

Citations

16
Pharmacological Properties and Molecular Targets of Alisol Triterpenoids from Alismatis Rhizoma DOI Creative Commons
Christian Bailly

Biomedicines, Journal Year: 2022, Volume and Issue: 10(8), P. 1945 - 1945

Published: Aug. 11, 2022

More than 100 protostane triterpenoids have been isolated from the dried rhizomes of Alisma species, designated Alismatis rhizoma (AR), commonly used in Asian traditional medicine to treat inflammatory and vascular diseases. The main products are alisols, with lead compounds alisol-A/-B their acetate derivatives being most abundant plant best-known bioactive products. pharmacological effects Ali-A, Ali-A 24-acetate, Ali-B, Ali-B 23-acetate, analyzed provide an overview medicinal properties, signaling pathways, molecular targets at origin those activities. Diverse protein proposed for these natural products, including farnesoid X receptor, soluble epoxide hydrolase, other enzymes (AMPK, HCE-2) functional proteins (YAP, LXR) anti-atherosclerosis, anti-inflammatory, antioxidant, anti-fibrotic, anti-proliferative Activities were classified two groups. lipid-lowering anti-atherosclerosis benefit robust vitro vivo data (group 1). anticancer alisols largely reported, but, essentially, studies using tumor cell lines solid lacking 2). survey shed light on properties alisol frequently found phytomedicines.

Language: Английский

Citations

15