Danubian Animal Genetic Resources,
Journal Year:
2023,
Volume and Issue:
8(2), P. 65 - 72
Published: Aug. 15, 2023
Zemplin
Rabbit
and
Liptov
Bold-Spotted
are
Slovak
national
breeds.
Our
aim
was
to
characterize
the
sperm
quality
of
these
two
breeds,
as
characteristics
important
for
artificial
insemination
cryopreservation
biodiversity
conservation
tools.
For
this
evaluation,
samples
sexually
mature
(ZR)
males
(n
=
6)
Rabbits
(LR)
4)
were
used.
According
progressive
motility
(PM)
data
(CASA),
divided
into
groups:
A
(>30%
PM)
B
(<30%
PM).
In
addition
PM
(ZR-A:
48.6±3.8%,
ZR-B:
16±3.2%,
LR-A:
35.38±2.6%,
LR-B:
4±2.2%),
total
(TM)
69.1±4.1%,
35.5±4.1%,
59.1±3%,
26.9±4.1%)
morphological
abnormalities
29.7±1.2%,
40±4%,
34.3±4.3%,
48.3±4.7%)
also
assessed
using
CASA
system.
The
proportion
dead/live,
apoptotic
oxidative
damaged
spermatozoa
(Spz)
by
flow
cytometry
fluorescent
dyes:
SYBR-14,
Sytox
Green,
Yo-Pro-1
CellROX
respectively.
results
correspond
values
morphometry.
Sperm
with
less
than
30%
does
not
show
proper
values,
while
higher
is
suitable
further
analysis
use.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Nov. 11, 2024
The
causes
of
variation
in
the
success
laparoscopic
artificial
insemination
(AI)
sheep
are
not
well
understood.
As
such,
this
study
incorporated
contributions
multiple
male
and
female
factors
relevant
to
AI
into
a
comprehensive
prediction
model
for
pregnancy
success.
Data
from
Merino
ewes
(N
=
30
254)
including
age,
uterine
tone
(1;
pale/flaccid-5;
turgid/pink),
intra-abdominal
fat
little
no
present-5;
high
fat),
time
sire
used,
were
recorded
during
AI.
A
subset
semen
per
388)
was
thawed
assessed
volume,
subjective
motility,
sperm
concentration,
morphology.
Sperm
motility
(CASA),
viability
acrosome
integrity
(FITC-PNA/PI),
membrane
fluidity
(M540/Yo-Pro),
mitochondrial
superoxide
production
(Mitosox
Red/Sytox
Green),
lipid
peroxidation
(Bodipy
C11),
level
intracellular
reactive
oxygen
species
(H
Antioxidants,
Journal Year:
2024,
Volume and Issue:
13(11), P. 1360 - 1360
Published: Nov. 6, 2024
Cryopreservation
plays
a
critical
role
in
animal
breeding
and
the
conservation
of
endangered
species,
but
it
often
compromises
sperm
characteristics
such
as
morphology,
motility,
viability
due
to
oxidative
stress.
This
study
explores
antioxidative
effect
Mito-Tempo
(MT)
Berberine
(BER)
enhance
post-thaw
quality
rabbits.
Pooled
rabbit
samples
were
supplemented
with
different
concentrations
(0.0,
0.5,
5,
10,
50
µmol/L)
MT
BER.
Sperm
motility
was
evaluated
using
computer-assisted
semen
analysis,
while
viability,
apoptosis,
reactive
oxygen
species
(ROS)
levels,
acrosome
integrity,
mitochondrial
function
assessed
through
flow
cytometry.
The
results
revealed
that
at
5
10
µmol/L
BER
significantly
improved
total
progressive
activity,
compared
control
group.
Furthermore,
enhanced
both
effectively
reduced
ROS
levels
apoptosis.
is
first
demonstrate
protective
effects
on
during
cryopreservation.
By
mitigating
stress
reducing
these
antioxidants
markedly
quality,
positioning
promising
agents
for
improving
cryosurvival.
Czech Journal of Animal Science,
Journal Year:
2024,
Volume and Issue:
69(3), P. 110 - 117
Published: March 7, 2024
Cryopreservation
reduces
the
antioxidant
activity
of
spermatozoa
and
makes
them
more
sensitive
to
damage
caused
by
reactive
oxygen
species
(ROS).
The
addition
antioxidants
freezing
medium
could
prevent
cryo-damage
mitigating
harmful
effects
ROS
and,
thus,
protecting
spermatozoa.
This
study
aimed
evaluate
effect
ellagic
acid
(EA)
on
rabbit
sperm
traits
after
freezing-thawing.
Semen
samples
collected
from
New
Zealand
White
males
were
cryopreserved
in
a
BotuCrio
(Nidacon,
Sweden)
supplemented
with
different
concentrations
(EA
at
0,
0.5,
1.5
2.5
mM)
using
manual
slow
procedure.
After
thawing,
motility
parameters
evaluated
CASA.
viability
(DRAQ7),
apoptosis
(Yo-Pro-1),
acrosome
integrity
(peanut
agglutinin;
PNA),
intracellular
(CellROX)
mitochondrial
(MitoTracker)
flow
cytometry.
EA
added
all
led
significant
reduction
(P
<
0.05)
frozen-thawed
cells.
However,
this
was
not
reflected
parameters.
mM
also
yielded
lower
proportion
apoptotic
cells
compared
control
group.
In
conclusion,
supplementation
semen
extender
demonstrated
its
antioxidative
properties
against
oxidative
during
cryopreservation.
Nevertheless,
draw
definitive
conclusion
regarding
functionality,
additional
research
is
necessary.
Veterinary Sciences,
Journal Year:
2023,
Volume and Issue:
10(7), P. 446 - 446
Published: July 8, 2023
The
present
study
aimed
to
evaluate
the
effects
of
melatonin
(MLT),
glutathione
(GSH),
and
their
combination
on
ram
semen
quality
after
thawing.
During
eight
weekly
sessions,
from
three
Merino
rams
was
pooled,
diluted
with
an
egg-yolk-based
extender,
divided
into
four
groups:
control,
1
mM
MLT,
5
GSH,
MLT
+
GSH.
Diluted
evaluated
before
freezing
process.
supplementation
GSH
at
had
a
deleterious
effect
total
motility
progressive
(TPM),
curvilinear
velocity
(VCL),
straight-line
(VSL),
average-path
(VAP),
linearity
(LIN),
straightness
(STR)
increased
slow
spermatozoa
(%).
also
negative
TPM,
VSL,
STR
in
semen.
In
thawed
semen,
TPM
compared
control
group.
VSL
lower
group
than
Additionally,
both
antioxidants
attenuated
BCF.
These
results
indicate
that
impairs
or
does
not
improve
sperm
kinetic
parameters
either
They
suggest
combined
plays
protective
role
against
these
effects.
Indian Journal of Animal Research,
Journal Year:
2024,
Volume and Issue:
Of
Published: March 14, 2024
Background:
Sperm
quality
plays
an
important
role
in
the
animal
industry.
The
study
of
sperm
Congjiang
Xiang
pigs
is
limited
and
Rh123/PI
double
staining
has
not
been
used
to
detect
boars.
Thus,
aimed
evaluate
feasibility
flow
cytometry
(FCM)
method
assessing
pigs.
Methods:
Semen
samples
were
evaluated
for
routine
indicators
semen
using
FCM
with
single
analyze
integrity
membrane
acrosome,
mitochondrial
function.
was
compared
Large
White
Result:
had
a
lower
concentration
than
that
(P
less
0.01).
There
no
significant
differences
vitality,
motility
abnormality
between
two
breeds
greater
0.05).
Single
revealed
acrosomal
No
difference
function
observed
demonstrated
rate
live
normal
significantly
higher
necrotic
sperms
Correlation
analysis
positive
association
rates
congjiang
xiang
their
(P=0.051).
Our
results
suggest
can
provide
more
reliable
information
assess
boar
quality.
Czech Journal of Animal Science,
Journal Year:
2024,
Volume and Issue:
69(5), P. 191 - 199
Published: May 30, 2024
This
research
investigates
the
high
prevalence
of
morphological
defects
in
sperm
a
particular
rabbit
breed,
namely
Zemplin
(ZR).
We
tried
to
uncover
possible
causes
and
consequences
observed
abnormalities.
Ejaculate
samples
from
ten
adult
males
were
divided
into
two
qualitative
groups
according
motility
parameters
revealed
by
CASA
(computer-assisted
analysis).
Group
A
contained
with
total
(TM)
above
50%
progressive
(PM)
30%.
B
TM
PM
below
these
values.
Viability
(SYBR-14/DRAQ5TM
SYTOXTM
Green/DRAQ5TM
staining)
apoptosis
(YO-PRO
TM-1/DRAQ5
stainig)
analyses
through
flow
cytometry
served
as
an
additional
insight
characteristics
groups.
In
both
cases,
we
noted
significant
differences
(P
<
0.05)
between
which
corresponded
Both
showed
increased
incidence
abnormal
spermatozoa,
did
not
observe
any
them.
Simultaneously,
assessed
practical
implications
on
artificial
insemination
(AI),
also,
notice
parameters.
The
use
transmission
electron
microscopy
enhanced
our
understanding
at
ultrastructural
level.
summary,
study
provides
semen,
highlighting
variations
quality
While
exist,
they
may
necessarily
translate
AI
outcomes.
Our
shows
higher
values
cytoplasmic
droplets
ZR
but
it
also
that
conditions
do
mean
fundamental
obstacle
fertility
case
AI.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 9, 2024
Abstract
The
causes
of
variation
in
the
success
laparoscopic
artificial
insemination
(AI)
sheep
are
not
well
understood.
As
such,
this
study
incorporated
contributions
multiple
male
and
female
factors
relevant
to
AI
into
a
comprehensive
prediction
model
for
pregnancy
success.
Data
from
Merino
ewes
(N
=
30
254)
including
age,
uterine
tone
(1;
pale/flaccid-5;
turgid/pink),
intra-abdominal
fat
little
no
present-5;
high
fat),
time
sire
used,
were
recorded
during
AI.
A
subset
semen
per
388)
was
thawed
assessed
volume,
subjective
motility,
sperm
concentration,
morphology.
Sperm
motility
(CASA),
viability
acrosome
integrity
(FITC-PNA/PI),
membrane
fluidity
(M540/Yo-Pro),
mitochondrial
superoxide
production
(Mitosox
Red/Sytox
Green),
lipid
peroxidation
(Bodipy
C11),
level
intracellular
reactive
oxygen
species
(H2DCFDA)
DNA
fragmentation
(Acridine
Orange)
also
0,
3
6h
post-thaw.
Logistic
binomial
regression
revealed
concentration
(P
<
0.001),
CASA
parameters
at
0h
(PCA3;
P
0.03),
viable
intact
0.02),
abnormal
morphology
0.001)
0.03)
influenced
likelihood
pregnancy.
Results
generated
will
help
standardise
pre-screening
selection
prior
breeding
programs,
reducing
