International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 24(1), P. 58 - 58
Published: Dec. 21, 2022
RNA-binding proteins are everywhere and accompany RNA molecules at every stage of their molecular life, from “birth” (transcription) through “growing up” (maturation), “active life” (molecular function) until “death” (turnover) [...]
Language: Английский
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: April 18, 2024
In eukaryotic cells, transcription, translation, and mRNA degradation occur in distinct subcellular regions. How these processes are organized bacteria, without employing membrane-bound compartments, remains unclear. Here, we present generalizable principles underlying coordination between bacteria.
Language: Английский
Citations
6
Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 52(7), P. 3870 - 3885
Published: March 7, 2024
Abstract The canonical stop codons of the nuclear genome trypanosomatid Blastocrithidia nonstop are recoded. Here, we investigated effect this recoding on mitochondrial and gene expression. Trypanosomatids possess a single mitochondrion protein-coding transcripts require RNA editing in order to generate open reading frames many encoded as ‘cryptogenes’. Small RNAs that can number hundreds direct produce transcriptome unusual complexity. We find B. have typical genetic code, which presumably requires disable utilization two nucleus-encoded suppressor tRNAs, appear be imported into organelle. Alterations protein factors responsible for mRNA were also documented, but they likely originated from sources other than recoding. population guide directing is minimal, yet virtually all genes plethora known still present. Most intriguingly, despite lacking complex I cryptogene RNAs, these stochastically edited high levels.
Language: Английский
Citations
5
Nucleic Acids Research, Journal Year: 2025, Volume and Issue: 53(4)
Published: Feb. 7, 2025
Human polynucleotide phosphorylase (hPNPase) is a 3'-to-5' exoribonuclease located in mitochondria, where it plays crucial roles RNA degradation and import. Mutations hPNPase can impair these functions, leading to various mitochondrial dysfunctions diseases. However, the mechanisms by which switches between its as an RNA-degrading enzyme carrier, well how disease-associated mutations may affect distinct remain unclear. In this study, we present cryo-electron microscopy structures of hPNPase, highlighting flexibility S1 domains, cap ring-like RNA-degradation chamber shift two distinctive open closed conformations. We further demonstrate small-angle X-ray scattering biochemical analyses that P467S G499R hPNPase's stem-loop RNA-binding activities limiting domain's ability transition from state. Conversely, D713Y mutation, within domain, does not affinity but diminishes interaction with Suv3 helicase for cooperative structured RNA. Collectively, findings underscore critical role domain mobility capturing import, involvement degradosome assembly. Our study thereby reveals molecular mechanism binding degradation, multiple defects could be induced disease-linked hPNPase.
Language: Английский
Citations
0
Seed Biology, Journal Year: 2023, Volume and Issue: 2(1), P. 0 - 0
Published: Jan. 1, 2023
Male reproduction, an essential and vulnerable process in the plant life cycle, is easily disrupted by changes surrounding environmental factors such as temperature, photoperiod, or humidity. Plants have evolved multiple mechanisms to buffer adverse effects; understanding these crucial increase crop resilience a changing climate, provide new breeding tools for hybrid seed production. Here, we review latest research progresses molecular underlying environment-sensitive genic male sterility fertility restoration, covering both genetic epigenetic aspects, summarize common conversion using knowledges obtained from photoperiod/thermo-sensitive (P/TGMS) mutants. This review, therefore, aims better understand adaptation response factors, with focus on future applications two-line breeding.
Language: Английский
Citations
8
Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(5), P. 588 - 588
Published: April 26, 2024
A wide variety of natural bioactive compounds derived from plants have demonstrated significant clinical relevance in the treatment various diseases such as cancer, chronic disease, and inflammation. An increasing number studies surfaced that give credence to potential plant-derived vesicle-like nanoparticles (PDVLNs) compelling candidates for a drug delivery system (DDS). PDVLNs are cost-effective production, non-toxicity non-immunogenicity fascinating bi-ocompatibility. In this review, we attempt comprehensively review consolidate position next-generation nanoplatforms. We aim quick glance readers current developments PDVLNs, including their biogenesis, characteristic features, composition, administration routes, advantages, application. Further, discuss advantages limitations PDVLNs. expect role will be significantly enhanced, thus positioning them next generation therapeutic modalities foreseeable future.
Language: Английский
Citations
3
IUBMB Life, Journal Year: 2023, Volume and Issue: 76(1), P. 38 - 52
Published: Aug. 18, 2023
Yeast mitochondrial genes are expressed as polycistronic transcription units that contain RNAs from different classes and show great evolutionary variability. The promoters simple, transcriptional control is rudimentary. Posttranscriptional mechanisms involving RNA maturation, stability, degradation thus the main force shaping transcriptome determining expression levels of individual genes. Primary transcripts fragmented by tRNA excision RNase P tRNase Z, additional processing events occur at dodecamer site 3' end protein-coding sequences. groups I II introns excised in a self-splicing reaction supported protein splicing factors encoded nuclear genes, or themselves. 3'-to-5' exoribonucleolytic complex called mtEXO activity involved turnover processing, an auxiliary 5'-to-3' exoribonuclease Pet127p. tRNAs and, to lesser extent, rRNAs undergo several base modifications. This gene system relies on coordinated action undergoes rapid evolution, contributing speciation events. Moving beyond classical model yeast Saccharomyces cerevisiae other budding yeasts should provide important insights into coevolution both genomes constitute eukaryotic genetic system.
Language: Английский
Citations
6
International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 24(1), P. 58 - 58
Published: Dec. 21, 2022
RNA-binding proteins are everywhere and accompany RNA molecules at every stage of their molecular life, from “birth” (transcription) through “growing up” (maturation), “active life” (molecular function) until “death” (turnover) [...]
Language: Английский
Citations
5