Mechanism of Mepiquat Chloride Regulating Soybean Response to Drought Stress Revealed by Proteomics DOI Creative Commons
Shoukun Dong,

Xin Wang,

Xiaomei Li

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(10), P. 2037 - 2037

Published: May 19, 2023

Soybeans are the main sources of oil and protein for most global population. As population grows, so does demand soybeans. However, drought is a major factor that limits soybean production. Regulating response to stress using mepiquat chloride (MC) feasible method; however, its mechanism still unclear. This study used PEG-6000 simulate quantitative proteomic techniques reveal changes in Heinong44 (HN44) Heinong65 (HN65) subjected following application 100 mg/L MC. The results showed SOD HN44 did not change significantly but decreased by 22.61% HN65 after MC pretreatment, MDA content 22.75% 21.54% HN65, respectively. Furthermore, improved GSH–ASA cycle simultaneously promoted Calvin process enable plant maintain certain carbon assimilation rate under osmotic stress. In addition, upregulated some proteins during gluconeogenesis starch metabolism increased soluble sugar 8.41% HN44. also reduced ribosomal abundance, affecting translation amino acid metabolism. summary, alleviate oxidative damage crop growth. Our first report regulation stress, providing new insights rational soybean.

Language: Английский

Biochemical characterization and metabolic reprogramming of amino acids in Soybean roots under drought stress DOI
Xiyue Wang, Xiaomei Li, Shoukun Dong

et al.

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(3)

Published: May 1, 2024

Abstract Amino acids play important roles in stress resistance, plant growth, development, and quality, with roots serving as the primary organs for drought response. We conducted biochemical multi‐omics analyses to investigate metabolic processes of root amino drought‐resistant (HN44) drought‐sensitive (HN65) soybean ( Glycine max ) varieties. Our analysis revealed an increase total acid content both varieties, phenylalanine, proline, methionine accumulating both. Additionally, several exhibited significant decreases HN65 but slight increases HN44. Multi‐omics association identified 13 acid‐related pathways. thoroughly examined changes genes metabolites involved various metabolism/synthesis determined core through correlation networks. The tyrosine, tryptophan pathways glutamic sulfur‐containing were particularly resistance. Some candidate genes, such ProDH P4HA family metabolites, O ‐acetyl‐L‐serine, directly affected up‐ downstream metabolism induce This study provided a basis resistance breeding.

Language: Английский

Citations

5
Transcriptomic and Metabolomic Analyses Reveal That Fullerol Improves Drought Tolerance in Brassica napus L DOI Open Access

Junlan Xiong,

Ni Ma

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(23), P. 15304 - 15304

Published: Dec. 4, 2022

Carbon nanoparticles have potential threats to plant growth and stress tolerance. The polyhydroxy fullerene-fullerol (one of the carbon nanoparticles) could increase biomass accumulation in several plants subjected drought; however, underlying molecular metabolic mechanisms governed by fullerol improving drought tolerance Brassica napus remain unclear. In present study, exogenous was applied leaves B. seedlings under conditions. results transcriptomic metabolomic analyses revealed changes profiles napus. differentially expressed genes accumulated metabolites, induced or treatment, were mainly enriched Kyoto Encyclopedia Genes Genomes (KEGG) pathways related carbohydrate metabolism (e.g., "carbon metabolism" "galactose metabolism"), amino acid "biosynthesis acids" "arginine proline secondary metabolite metabolites"). For metabolism, oligosaccharides sucrose) decreased, whereas that monosaccharides mannose myo-inositol) increased drought. With regard stress, acids such as phenylalanine tryptophan glutamate increased. Further, for soil drying showed a reduction phenolics flavonoids, hyperoside trans-3-coumaric acid. However, carbohydrates almost unchanged fullerol-treated When exposed water shortage, acids, proline, decreased upon treatment. luteolin acid, enhanced. Our findings suggest can alleviate inhibitory effects on flavonoids enhance

Language: Английский

Citations

22
Screening and identification of drought tolerance of spring soybean at seedling stage under climate change DOI Creative Commons
Xin Wang, Xiaomei Li, Shoukun Dong

et al.

Frontiers in Sustainable Food Systems, Journal Year: 2022, Volume and Issue: 6

Published: Aug. 15, 2022

Drought is one of the major abiotic stress factors limiting soybean growth and yield, it frequently occur globally. Therefore, exploring resistant varieties from germplasm important under climate change. To screen drought spring at seedling stage, pot experiment was used to detect Survival percentage after 60 twice rehydration treatments on seedlings, evaluate tolerance soybean. The results showed that seven were considered tolerant, 17 sensitive, 36 be moderately tolerant. Based this experiment, number 44 (heinong37), 48 (heinong44), 49 (heinong45), 52 (heinong48) best resistant, 3 (dongnong48), 4 (dongnong52), 27 (suinong25), (heinong65) most sensitive. These provide a reference for further study resistance gene screening molecular level. selected can planted in areas with suitable climates frequent meet local demand. In other regions, although cannot directly grown, they still as parents or materials extraction, assist crop breeding level response increasingly severe problems current trends.

Language: Английский

Citations

21
Regulation of soybean drought response by mepiquat chloride pretreatment DOI Creative Commons
Xiyue Wang,

Xinyu Zhou,

Zhipeng Qu

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: May 8, 2023

Soybean is the world's most important cultivated crop, and drought can affect their growth and, eventually, yields. Foliar application of mepiquat chloride (MC) potentially alleviate damage caused by stress in plants; however, mechanism MC regulation soybean response has not been studied. This study investigated two varieties soybean, sensitive Heinong 65 (HN65) drought-tolerant Heinong44 (HN44), under three treatment scenarios, normal, stress, + conditions. promoted dry matter accumulation reduced plant height, decreased antioxidant enzyme activity, significantly malondialdehyde content. The light capture processes, photosystems I II, were inhibited; upregulation several amino acids flavonoids was observed. Multi-omics joint analysis indicated 2-oxocarboxylic acid metabolism isoflavone biosynthetic pathways to be core which regulated response. Candidate genes such as LOC100816177, SOMT-2, LOC100784120, LOC100797504, LOC100794610, LOC100819853 identified crucial for resistance soybeans. Finally, a model constructed systematically describe regulatory stress. fills research gap field resistance.

Language: Английский

Citations

11
Integrated transcriptome and metabolome analysis reveals the response mechanisms of soybean to aluminum toxicity DOI
Huiwen Zhou,

Lanhua Wu,

Ruikai Wang

et al.

Plant and Soil, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Language: Английский

Citations

0
Integrative metabolomics and transcriptomics profiling reveals differential expression of flavonoid synthesis in Ophiopogon japonicus (L. f.) Ker-Gawl. in adaptation to drought DOI Creative Commons
Tingting Cheng, Juan Lin,

Xia Zhou

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0313580 - e0313580

Published: Jan. 7, 2025

Drought is one of the consequences climate change that severely affects plant growth and development. Ophiopogon japonicus (L. f.) Ker-Gawl. (Chinese name: Chuanmaidong, abbreviated as CMD) a commonly used herbaceous whose development are strongly affected by drought. Here, we comprehensively analyzed transcriptomic metabolic responses two CMD varieties (EP CP) to drought stress. CP utilized small number differentially expressed genes regulate greater differential metabolites compared EP, suggesting it may be more tolerant. In addition, integrated transcriptome metabolome analyses revealed transcription factors such WRKY, TIFY, C2H2 flavonoid synthesis in CMD. These findings provide ideas for in-depth analysis mechanism against stress, theoretical basis breeding high-quality drought-tolerant varieties.

Language: Английский

Citations

0
The PmCHS and PmANR of Pinus massoniana regulate flavonoid metabolism in response to high temperatures and drought DOI Creative Commons
Liangliang Li, Honglang Duan, Zhiyong Li

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 225, P. 120495 - 120495

Published: Jan. 21, 2025

Language: Английский

Citations

0
Analysis of Volatile Metabolome and Transcriptome in Sweet Basil Under Drought Stress DOI Creative Commons
Yuan Zhou, Guangying Ma,

Wenlue Li

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(2), P. 117 - 117

Published: Feb. 11, 2025

Basil, renowned for its aromatic properties, exhibits commendable drought tolerance and holds significant value as an edible medicinal plant. Recognizing the scarcity of studies addressing basil’s response to stress, we performed physiological experiments omics analyses sweet basil across four distinct levels stress. During showed increased activity antioxidant enzymes accumulated more osmoregulatory compounds. Our metabolic analysis meticulously identified a total 830 metabolites, among which, 215 were differentially accumulated. The metabolites under stress predominantly esters terpenes; however, none primary volatile compounds basil. Transcriptome highlighted pivotal roles phenylpropanoid flavonoid biosynthesis lipid metabolism in fortifying resistance against α-linolenic acid, lignin, flavonoid, flavonol contents significantly stress; essential genes involved production these confirmed through quantitative real-time PCR (qRT-PCR), their variations aligned with outcomes from sequencing. This holistic approach not only enriches our understanding molecular intricacies underpinning but also furnishes valuable insights breeding varieties endowed enhanced tolerance.

Language: Английский

Citations

0
Physiological and Gene Expression Responses in Soybean Under Situations of Water Deficits DOI

Ch. Aruna Kumari,

V. Ramya,

M. Madhavi

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Dual Melatonin Enhances Coordination Between Carbon and Nitrogen Assimilation in Soybean DOI Creative Commons
Yanhong Wang, Xijun Jin, Yuxian Zhang

et al.

Agriculture, Journal Year: 2025, Volume and Issue: 15(7), P. 681 - 681

Published: March 23, 2025

Soybean production is currently insufficient to meet global demand, highlighting the need for strategies enhance growth. Melatonin (MT) has emerged as a promising solution due its growth-promoting properties. This study investigated effects of dual MT treatment—combining seed soaking and foliar spraying—on soybean carbon nitrogen metabolism using metabolomics analysis. The results demonstrated that treatment significantly upregulated TCA cycle, providing energy precursors amino acid carbohydrate synthesis. Key pathways, including histidine phenylalanine metabolism, were enhanced, with stimulating purine synthesis improve biological fixation promoting secondary metabolite support Additionally, pathways such starch sucrose glycolysis/gluconeogenesis positively regulated, ensuring supply homeostasis. Overall, enhanced metabolic capacity by production, maintaining balance. These findings underscore melatonin’s regulatory role in growth provide insights improving crop productivity.

Language: Английский

Citations

0