New insights into short-term water stress tolerance through transcriptomic and metabolomic analyses on pepper roots

Plant Science, Journal Year: 2023, Volume and Issue: 333, P. 111731 - 111731

Published: May 15, 2023

In the current climate change scenario, water stress is a serious threat to limit crop growth and yields. It necessary develop tolerant plants that cope with and, for this purpose, tolerance mechanisms should be studied. NIBER® proven stress- salt-tolerant pepper hybrid rootstock (Gisbert-Mullor et al., 2020; López-Serrano 2020), but remain unclear. experiment, A10 (a sensitive accession (Penella 2014)) response short-term at 5 h 24 was studied in terms of gene expression metabolites content roots. GO analyses evidenced constitutive differences transcriptomic profile A10, associated detoxification systems reactive oxygen species (ROS). Upon stress, transcription factors like DREBs MYC are upregulated levels auxins, abscisic acid jasmonic increased NIBER®. involve an increase osmoprotectant sugars (i.e., trehalose, raffinose) antioxidants (spermidine), lower contents oxidized glutathione compared which indicates less oxidative damage. Moreover, aquaporins chaperones enhanced. These results show main strategies overcome stress.

Language: Английский

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Physiological responses and transcriptome analysis of soybean under gradual water deficit

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 26, 2023

Soybean is an important food and oil crop widely cultivated globally. However, water deficit can seriously affect the yield quality of soybeans. In order to ensure stability increase soybean improve agricultural use efficiency (WUE), research on improving drought tolerance utilization soybeans under stress has become particularly important. This study utilized drought-tolerant variety Heinong 44 (HN44) drought-sensitive Suinong 14 (SN14) analyze physiological responses transcriptome changes during gradual at early seed-filling stage. The results indicated that conditions, HN44 had smaller stomata, higher stomatal density, lower conductance (Gs) transpiration rate as compared SN14. Additionally, a abscisic acid (ABA) content faster in morphology Gs maintain dynamic balance between net photosynthetic (Pn) Gs. high instantaneous WUE deficit. Further, retained level superoxide dismutase (SOD) activity proline content, mitigating malondialdehyde (MDA) accumulation drought-induced damage. Comprehensive analysis data revealed fewer differentially expressed genes (DEGs) light stress, reacting insensitivity At initial stage both varieties large number upregulated DEGs cope with stress. Under severe downregulated enriched photosynthesis pathway than SN14, while it more ABA-mediated signaling glutathione metabolism pathways During deficit, demonstrated better characteristics SN14 through key such GmbZIP4, LOC100810474, LOC100819313 major pathways. Key transcription factors were screened identified, providing further clarity molecular regulatory responsible for differences among these varieties. deepened understanding resistance mechanisms soybeans, valuable references drought-resistant breeding.

Language: Английский

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Natural variation of GmFNSII-2 contributes to drought resistance by modulating enzyme activity in soybean

The Crop Journal, Journal Year: 2024, Volume and Issue: 12(2), P. 529 - 539

Published: March 13, 2024

As an essential crop that provides vegetable oil and protein, soybean (Glycine max (L.) Merr.) is widely planted all over the world. However, scarcity of water resources worldwide has seriously impacted on quality yield soybean. To address this, exploring excellent genes for improving drought resistance in crucial. In this study, we identified natural variations GmFNSII-2 (flavone synthase II) significantly affect soybeans. Through sequence analysis 632 cultivated 44 wild soybeans nine haplotypes were identified. The full-length allele GmFNSII-2C, but not truncated GmFNSII-2A possessing a nonsense nucleotide variation, increased enzyme activity. Further research found GmDREB3, known to increase resistance, bound promoter region GmFNSII-2C. GmDREB3 positively regulated expression flavone abundance improved resistance. Furthermore, single-base mutation GmFNSII-2C generated additional response element (CCCCT), which had stronger interaction strength with its transcriptional activity under conditions. frequency drought-resistant varieties Hap 1 (Pro:GmFNSII-2C) increased, suggesting haplotype may be selected during breeding. summary, could used molecular breeding drought-tolerant

Language: Английский

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Variability of Root and Shoot Traits under PEG-Induced Drought Stress at an Early Vegetative Growth Stage of Soybean

Agronomy, Journal Year: 2024, Volume and Issue: 14(6), P. 1188 - 1188

Published: May 31, 2024

The performance of a soybean genotype under water deficit stress at an early vegetative stage might be related to its general tolerance drought. To investigate the plasticity root and shoot seedling traits in response drought stage, set 32 genotypes adapted southeast European growing conditions was grown polyethylene glycol (PEG) 8000-induced well-watered control conditions. Under drought, mean tap length (RL), (SL), fresh weight (RFW) (SFW) decreased significantly by 11, 17, 38 34%, respectively, while dry matter (RDM) (SDM) increased 13 11%, respectively. Of four derived traits, ratios RL/SL, RL/RFW SL/SFW 8, 45 28%, ratio RFW/SFW 4%. However, wide variation between observed for all 10 studied both Broad sense heritability ranged from 0.53 (RL) 0.97 (SL) 0.56 (RL/RFW) 0.96 correlation coefficients were either weak or moderate, indicating that can modified independently selection.

Language: Английский

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Mechanism of Mepiquat Chloride Regulating Soybean Response to Drought Stress Revealed by Proteomics

Plants, Journal Year: 2023, Volume and Issue: 12(10), P. 2037 - 2037

Published: May 19, 2023

Soybeans are the main sources of oil and protein for most global population. As population grows, so does demand soybeans. However, drought is a major factor that limits soybean production. Regulating response to stress using mepiquat chloride (MC) feasible method; however, its mechanism still unclear. This study used PEG-6000 simulate quantitative proteomic techniques reveal changes in Heinong44 (HN44) Heinong65 (HN65) subjected following application 100 mg/L MC. The results showed SOD HN44 did not change significantly but decreased by 22.61% HN65 after MC pretreatment, MDA content 22.75% 21.54% HN65, respectively. Furthermore, improved GSH–ASA cycle simultaneously promoted Calvin process enable plant maintain certain carbon assimilation rate under osmotic stress. In addition, upregulated some proteins during gluconeogenesis starch metabolism increased soluble sugar 8.41% HN44. also reduced ribosomal abundance, affecting translation amino acid metabolism. summary, alleviate oxidative damage crop growth. Our first report regulation stress, providing new insights rational soybean.

Language: Английский

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