Biomedicine & Pharmacotherapy,
Journal Year:
2023,
Volume and Issue:
169, P. 115937 - 115937
Published: Nov. 25, 2023
Alveolar
epithelial
cell
(AEC)
senescence
is
considered
to
be
a
universal
pathological
feature
of
many
chronic
pulmonary
diseases.
Our
previous
study
found
that
epoxyeicosatrienoic
acids
(EETs),
produced
from
arachidonic
acid
(ARA)
through
the
cytochrome
P450
cyclooxygenase
(CYP)
pathway,
have
significant
negative
regulatory
effects
on
cellular
in
AECs.
However,
exact
mechanisms
by
which
EETs
alleviate
AECs
still
need
further
explored.
In
present
study,
we
observed
bleomycin
(BLM)
induced
enhanced
mitophagy
accompanied
increased
mitochondrial
ROS
(mito-ROS)
content
murine
alveolar
line
MLE12.
While
reduced
BLM-induced
and
mito-ROS
MLE12
cells,
mechanism
was
related
regulation
NOX4/Nrf2-mediated
redox
imbalance.
Furthermore,
inhibition
degradation
could
significantly
inhibit
regulate
NOX4/Nrf2
balance
exert
anti-oxidant
D-galactose-induced
premature
aging
mice.
Collectively,
these
findings
may
provide
new
ideas
for
treating
age-related
diseases
targeting
improve
dysfunction
reduce
oxidative
stress.
Frontiers in Endocrinology,
Journal Year:
2023,
Volume and Issue:
13
Published: Jan. 4, 2023
Backgrounds
Diabetic
retinopathy
(DR)
is
a
common
diabetic
ocular
disease
characterized
by
retinal
ganglion
cell
(RGC)
changes.
An
abnormal
environment,
hyperglycemia,
may
progressively
alter
the
structure
and
function
of
RGCs,
which
primary
pathological
feature
neurodegeneration
in
DR.
Accumulated
studies
confirmed
autophagy
senescence
play
vital
role
DR;
however,
underlying
mechanisms
need
to
be
clarified.
Methods
This
study
included
microarray
expression
profiling
dataset
GSE60436
from
Gene
Expression
Omnibus
(GEO)
conduct
bioinformatics
analysis.
The
R
software
was
used
identify
autophagy-related
genes
(ARGs)
that
were
differentially
expressed
fibrovascular
membranes
(FVMs)
normal
retinas.
Co-expression
tissue-specific
elicited
for
filtered
genes.
then
analyzed
ontology
(GO)
enrichment
analysis,
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
pathway
analysis
Set
Enrichment
Analysis
(GSEA).
R28
cells
cultured
with
high
glucose,
detected
reverse
transcription-quantitative
(RT-qPCR)
stained
apoptosis
kit.
Results
In
retina,
31
ARGs
(24
up-regulated
genes)
discovered
enriched.
results
revealed
significantly
enriched
autophagy,
apoptosis,
aging,
neural
function.
Four
hub
(i.e.,
TP53
,
CASP1
CCL2
)
up-regulated.
Upregulation
cellular
level
hyperglycemia
model
vitro
.
Conclusions
Our
provide
evidence
involved
injury,
protective
limited.
Further
favour
understanding
progression
neuroprotection
Acta Pharmaceutica Sinica B,
Journal Year:
2023,
Volume and Issue:
13(6), P. 2628 - 2644
Published: March 10, 2023
The
mechanisms
underlying
autophagic
defects
in
nonalcoholic
steatohepatitis
(NASH)
remain
largely
unknown.
We
aimed
to
elucidate
the
roles
of
hepatic
cyclooxygenase
1
(COX1)
autophagy
and
pathogenesis
diet-induced
mice.
Human
fatty
liver
disease
(NAFLD)
samples
were
used
examine
protein
expression
COX1
level
autophagy.
Cox1Δhepa
mice
their
wildtype
littermates
generated
fed
with
3
different
NASH
models.
found
that
was
increased
patients
models
accompanied
by
impaired
required
for
basal
hepatocytes
specific
deletion
exacerbated
inhibiting
Mechanistically,
directly
interacted
WD
repeat
domain,
phosphoinositide
interacting
2
(WIPI2),
which
crucial
autophagosome
maturation.
Adeno-associated
virus
(AAV)-mediated
rescue
WIPI2
reversed
flux
improved
phenotypes
mice,
indicating
deletion-mediated
partially
dependent
on
WIPI2-mediated
In
conclusion,
we
demonstrated
a
novel
role
protected
against
WIPI2.
Targeting
COX1-WIPI2
axis
may
be
therapeutic
strategy
NASH.
Biomedicine & Pharmacotherapy,
Journal Year:
2023,
Volume and Issue:
169, P. 115937 - 115937
Published: Nov. 25, 2023
Alveolar
epithelial
cell
(AEC)
senescence
is
considered
to
be
a
universal
pathological
feature
of
many
chronic
pulmonary
diseases.
Our
previous
study
found
that
epoxyeicosatrienoic
acids
(EETs),
produced
from
arachidonic
acid
(ARA)
through
the
cytochrome
P450
cyclooxygenase
(CYP)
pathway,
have
significant
negative
regulatory
effects
on
cellular
in
AECs.
However,
exact
mechanisms
by
which
EETs
alleviate
AECs
still
need
further
explored.
In
present
study,
we
observed
bleomycin
(BLM)
induced
enhanced
mitophagy
accompanied
increased
mitochondrial
ROS
(mito-ROS)
content
murine
alveolar
line
MLE12.
While
reduced
BLM-induced
and
mito-ROS
MLE12
cells,
mechanism
was
related
regulation
NOX4/Nrf2-mediated
redox
imbalance.
Furthermore,
inhibition
degradation
could
significantly
inhibit
regulate
NOX4/Nrf2
balance
exert
anti-oxidant
D-galactose-induced
premature
aging
mice.
Collectively,
these
findings
may
provide
new
ideas
for
treating
age-related
diseases
targeting
improve
dysfunction
reduce
oxidative
stress.
