Improvement of Seed Germination under Salt Stress via Overexpressing Caffeic Acid O-methyltransferase 1 (SlCOMT1) in Solanum lycopersicum L.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(1), P. 734 - 734

Published: Jan. 1, 2023

Melatonin (MT) is a phytohormone-like substance and profoundly involved in modulating nearly all aspects of plant development acclimation to environmental stressors. However, there remain no studies about the effects MT on tomato seed germination under salt stress. Here we reported that overexpression caffeic acid O-methyltransferase 1 (SlCOMT1) significantly increased both content tolerance germinated seeds transgenic relative wild type (WT) samples. Physiological investigation showed higher amylase activity stressed than WT, leading promoted starch decomposition enhanced soluble sugar content. The stimulated production osmolytes activities SOD, POD, CAT, together with significant reduction H2O2 O2·- accumulation, were revealed largely accounting for their lower membrane lipid peroxidation. qPCR assay that, upon stress, transcript abundance hub genes related (SlCYP707A1, SlABA1, SlGA3ox2 SlGA2ox4) stress (SlCDPK1, SlWRKY33 SlMAPK1) distinctly altered samples when compared providing molecular basis MT-mediated improvement tolerance. Altogether, our observations shed new insights into biological functions SlCOMT1 could expand its utilization genetic future.

Language: Английский

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Elucidating the modulatory effect of melatonin on enzyme activity and oxidative stress in wheat: a global meta‐analysis

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(2)

Published: March 1, 2024

Abstract In our comprehensive meta‐analysis, we initially collected 177 publications focusing on the impact of melatonin wheat. After meticulous screening, 40 published studies were selected, encompassing 558 observations for antioxidant enzymes, 312 reactive oxygen species (ROS), and 92 soluble biomolecules (soluble sugar protein). This analysis revealed significant heterogeneity across (I 2 > 99% ROS, biomolecules) notable publication bias, indicating complexity variability in research field. Melatonin application generally increased enzyme activities [superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate (APX)] wheat, particularly under stress conditions, such as high temperature heavy‐metal exposure. Compared to control, SOD, POD, CAT, APX by 29.5, 16.96, 35.98, 171.64%, respectively. Moreover, oxidative markers like hydrogen peroxide (H O ), superoxide anion (O malondialdehyde (MDA) decreased with 23.73, 13.64, 21.91%, respectively, suggesting a reduction stress. The also highlighted melatonin's role improving carbohydrate metabolism defenses. showed an overall increase 12.77% content, 22.76% glutathione (GPX) activity compared control. However, effects varied different wheat varieties, environmental methods. Our study uncovered complex relationships between H levels, nuanced regulatory responses. meta‐analysis demonstrates increasing resilience abiotic stressors, potentially through its defense systems response.

Language: Английский

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Weighted gene co-expression network analysis revealed the key pathways and hub genes of potassium regulating cotton root adaptation to salt stress

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: March 1, 2023

Soil salinization is one of the main abiotic stresses affecting cotton yield and planting area. Potassium application has been proven to be an important strategy reduce salt damage in agricultural production. However, mechanism potassium regulating adaptability not fully elucidated. In present research, appropriate rate for alleviating based on different K+/Na+ ratios we screened, a gene co-expression network weighted analysis (WGCNA) using transcriptome data sets treated with CK (0 mM NaCl), S (150 SK8 NaCl + 9.38 K2SO4) was constructed. this study, four key modules that are highly related regulation tolerance were identified, mitogen-activated protein kinase (MAPK) signaling pathway, tricarboxylic acid (TCA) cycle glutathione metabolism pathway identified as biological processes metabolic pathways improve root adaptability. addition, 21 hub genes 120 candidate suggesting they may play role enhancement by potassium. The modules, discovered study will provide new understanding molecular enhancing salinity cotton, lay theoretical foundation improvement innovation high-quality germplasm.

Language: Английский

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Exogenous serotonin improves drought and salt tolerance in tomato seedlings

Plant Growth Regulation, Journal Year: 2023, Volume and Issue: 101(1), P. 239 - 249

Published: May 22, 2023

Language: Английский

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Seed priming with melatonin improved salinity tolerance of halophytes during early life-cycle stages

Plant Growth Regulation, Journal Year: 2024, Volume and Issue: 103(2), P. 351 - 368

Published: Jan. 21, 2024

Language: Английский

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Physiological and Biochemical Mechanisms of Exogenous Melatonin Regulation of Saline–Alkali Tolerance in Oats

Agronomy, Journal Year: 2023, Volume and Issue: 13(5), P. 1327 - 1327

Published: May 9, 2023

Saline–alkali stress is one of the major factors limiting oat seed germination. The regulatory role melatonin (MT) as a naturally occurring active substance well known, but mechanism MT-mediated intrinsic physiological regulation germination under saline–alkali unclear. Therefore, this study investigated (1) variability different MT soaking concentrations and times on seeds stress, (2) possible mechanisms stress. results showed that vigor was significantly reduced oats inhibited; treatments improved rate, potential, index, root length, germ fresh weight, dry weight; and, overall, treatment exhibited phenomenon “low promotion high inhibition”. 100 μmol·L−1 effectively enhanced activities antioxidant enzymes (SOD, POD, CAT, APX) nonenzymatic antioxidants (GSH AsA), affected AsA-GSH cycle, increased contents osmoregulatory substances (proline, protein, soluble sugar) by reducing levels H2O2, OH−, MDA, thus enhancing tolerance to promoting In conclusion, has positive effect seeds.

Language: Английский

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Role of Melatonin in Directing Plant Physiology

Agronomy, Journal Year: 2023, Volume and Issue: 13(9), P. 2405 - 2405

Published: Sept. 18, 2023

Melatonin (MT), a naturally occurring compound, is found in various species worldwide. In 1958, it was first identified the pineal gland of dairy cows. MT an “old friend” but “new compound” for plant biology. It brings experts and research minds from broad field sciences due to its considerable influence on systems. The production process plants animals distinct, where has been expressed explicitly chloroplasts mitochondria plants. Tryptophan acts as precursor formation phyto-melatonin, along with intermediates including tryptamine, serotonin, N-acetyl 5-methoxy tryptamine. plays vital role growth phases such seed germination seedling crop significantly impacts gas exchange, thereby improving physio-chemical functions During stress, excessive generation accumulation reactive oxygen (ROS) causes protein oxidation, lipid peroxidation, nucleic acid damage, enzyme inhibition. Because directly antioxidant awakens defense system during stress reduces ROS, which results decreasing cellular oxidative damage. can enhance development response abiotic stresses drought, salinity, high temperature, flooding, heavy metals by regulating mechanism However, these reactions differ are based level kind stress. physiological towards development, tolerance stresses, approaches enhancing endogenous systems broadly reviewed suggested that steering compound directing major under changing climate future.

Language: Английский

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Hormonal Priming to Increase Germination of Stevia rebaudiana Bertoni Seeds in Saline Environments

International Journal of Plant Biology, Journal Year: 2025, Volume and Issue: 16(1), P. 2 - 2

Published: Jan. 2, 2025

Hormonal priming has recently emerged as a powerful strategy to increase seed germination and early seedling growth, especially in challenging abiotic stress environments. This study explored the impact of gibberellic acid (GA) salicylic (SA) on performance Stevia rebaudiana seeds under saline conditions. were either hydroprimed with distilled water (control) or primed varying concentrations GA (0.1 0.2%) SA (0.25 0.5 mM) then exposed salt (0 80 mM NaCl). The results demonstrated that significantly enhanced rates, reduced mean time, improved tolerance index compared untreated seeds. Primed showed notable improvements vigor, including greater shoot root lengths salinity stress. best achieved 0.1% SA, effectively alleviating detrimental high germination. also exhibited electrolyte leakage, signifying membrane stability In conclusion, this presents robust evidence is an effective approach for enhancing germination, index, growth conditions, offering practical solution improve crop establishment salinity-affected regions.

Language: Английский

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Efficacy and mechanisms of seed priming with melatonin to enhance salinity tolerance

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 137 - 156

Published: Jan. 1, 2025

Language: Английский

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Salicylic acid nanoliposomes treatment alleviates chilling injury in postharvest baby ginger by regulating membrane lipid metabolism

Food Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 144137 - 144137

Published: March 1, 2025

Language: Английский

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