Indicators of inflammatory, multiorgan impairment and redox biomarkers and CT findings in patients with COVID-19 pneumonia DOI Creative Commons
Tijana Kosanović, Dragan Sagić, Vesna Ćorić

et al.

Medicinski podmladak, Journal Year: 2024, Volume and Issue: 75(3), P. 1 - 8

Published: Jan. 1, 2024

Coronavirus disease 2019 (COVID-19) is a multi-systemic caused by Severe acute respiratory syndrome coronavirus 2, SARS-CoV-2. Since the onset of pandemic, understanding pathophysiological mechanisms this has posed significant challenge, with intent to determine its progression and implement appropriate treatment strategies. The heightened severity mortality associated SARS-CoV-2 infection can be attributed, in part, phenomenon known as cytokine storm. This refers an uncontrolled systemic inflammatory response characterized elevated proinflammatory cytokines chemokines, leading overproduction free radicals. aforementioned storm accompanied excessive generation reactive oxygen species affects virus directly damages host's cells. Given that primarily targets cells, pneumonia common manifestation disease. Consequently, chest multidetector computed tomography (MDCT) plays crucial role evaluating lung tissue inflammation, determining severity, making decisions regarding hospitalization, assessing necessity intensive care unit treatment. Assessing level oxidative stress accomplished measuring products resulting from damage lipids, proteins, DNA - whereas multiorgan impairment biomarkers procured routine laboratory practice. Due established association radical storm, it might postulated during phase COVID-19 redox correlate biomarkers, well MDCT findings.

Language: Английский

Albumin Is a Component of the Esterase Status of Human Blood Plasma DOI Open Access
Daria A. Belinskaia, Polina A. Voronina,

Polina I. Popova

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(12), P. 10383 - 10383

Published: June 20, 2023

The esterase status of blood plasma can claim to be one the universal markers various diseases; therefore, it deserves attention when searching for severity COVID-19 and other infectious non-infectious pathologies. When analyzing plasma, activity serum albumin, which is major protein in mammals, should not ignored. purpose this study expand understanding evaluate relationship status, includes information on amount enzymatic human albumin (HSA), with biochemical parameters blood, using example surviving deceased patients confirmed COVID-19. In experiments vitro silico, pure HSA towards substrates was studied, effect inhibitors tested. Then, a comparative analysis number basic healthy subjects performed. Statistically significant differences have been found indices (including levels) between COVID-19, as well patients. Additional evidence has obtained importance diagnostic marker. Of particular interest new index, [Urea] × [MDA] 1000/(BChEb [ALB]), group 10 times higher than survivors 26 value apparently elderly subjects.

Language: Английский

Citations

11
Small Ruminant Lentiviruses Induce Systemic and Joint Oxidative Stress in Dairy Goats with Chronic Osteoarthritis DOI

Dominique Esther Machado Ferr Marsili,

R. da S. Carvalho,

Beatriz Nascimento Lins de Oliveira

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Neurometabolomic impacts of wood smoke and protective benefits of anti-aging therapeutics in aged female C57BL/6J mice DOI Creative Commons
David Scieszka, Jonathan Hulse, Haiwei Gu

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Background Wildland fires have become progressively more extensive over the past 30 years in United States, routinely generating smoke that deteriorates air quality for most of country. We explored neurometabolomic impact biomass-derived on older (18 months) female C57BL/6J mice, both acutely and after 10 weeks recovery from exposures. Methods Mice were exposed to wood (WS) 4 hours/day, every other day, 2 (7 exposures total) an average concentration 448 µg particulate matter (PM)/m 3 per exposure. One group was euthanized 24 hours last Other groups then placed 1 treatment regimens exposures: vehicle; resveratrol chow plus nicotinamide mononucleotide water (RNMN); senolytics via gavage (dasatanib + quercetin; DQ); or RNMN with DQ (RNDQ). Results Among findings, aging 18 months 21 associated greatest metabolic shift, including changes metabolism, WS exposure effects relatively modest. caused a reduction NAD within prefrontal cortex immediately long-term serotonin persisted weeks. The reductions corroborated by behavioral changes, increased immobility forced swim test, neuroinflammatory markers had beneficial exposure, while RNDQ brain be upregulated WS-exposed mice. Discussion Taken together, these findings highlight persistent woodsmoke aged mouse model. Further examination is necessary determine age-specific species-determinant response pathways duration before complete resolution occurs.

Language: Английский

Citations

0
Relationship between red blood cell aggregation and dextran molecular mass DOI Creative Commons
Maciej Bosek, Blanka Ziomkowska, Jerzy Pyskir

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Nov. 17, 2022

The aim of this study was to investigate the aggregation red blood cells (RBCs) suspended in dextran solution at various levels molecular mass. Dextran solutions mass 40, 70, 100 and 500 kDa concentration from 2 5 g/dL were used suspend RBCs. radius velocity sedimenting RBC aggregates investigated using image analysis. sedimentation increased initially, then decreased after achieving maxima. maximal showed a bell-shaped dependence on concentration, whereas monotonic increase with both factors. Difference between aggregate density estimated viscosity, consistent across most levels. This allowed calculate porosity show that it monotonically density, caused by concentration. results provide insight into process proteins different size, reflecting pathological conditions. currently reported data can be potentially applied specific pathophysiological conditions giving an interpretation is not yet fully discussed literature.

Language: Английский

Citations

13
A Preliminary Evaluation of Advanced Oxidation Protein Products (AOPPs) as a Potential Approach to Evaluating Prognosis in Early-Stage Breast Cancer Patients and Its Implication in Tumour Angiogenesis: A 7-Year Single-Centre Study DOI Open Access
Marta Napiórkowska-Mastalerz, Tomasz Wybranowski, Maciej Bosek

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(5), P. 1068 - 1068

Published: March 6, 2024

Breast cancer (BrC) is a highly prevalent tumour among women. The high incidence and mortality rate of BrC prompts researchers to search for new markers that will provide information on the possible impact therapy risk cancer-related events. This study aimed investigate whether level advanced oxidation protein products (AOPPs) may have potential disease-free (DFS) overall survival (OS) in patients with early-stage cancer. Additionally, we tried assess relationship between AOPPs angiogenic parameters. In this study, pre- post-treatment AOPP levels were examined serum 70 newly diagnosed receiver operating characteristic curve identified be above 9.37 μM 10.39 μM, respectively, as best cut-off values predict relapse. Kaplan-Meier analysis indicated associated significantly poorer OS. uni- multivariate Cox regression highlighted lower longer duration without relapse or death. A positive correlation concentrations pre-treatment vascular endothelial growth factor A, negative correlations soluble forms receptor type 1 2, found. conclusion, appear an important role predicting events potentially serve simple prognostic marker clinical practice.

Language: Английский

Citations

2
Oxidative stress in the alveolar lavage fluid of children with Mycoplasma pneumoniae pneumonia DOI
Mengzhu Wang,

Ruijuan Ren,

Yuping Xu

et al.

Pediatric Pulmonology, Journal Year: 2024, Volume and Issue: 59(11), P. 2772 - 2782

Published: June 21, 2024

To investigate the correlation between oxidative stress in bronchoalveolar lavage fluid (BALF) of children with Mycoplasma pneumoniae pneumonia (MPP) and clinical characteristics severe MPP (SMPP) refractory (RMPP).

Language: Английский

Citations

2
Stable Nitroxide as Diagnostic Tools for Monitoring of Oxidative Stress and Hypoalbuminemia in the Context of COVID-19 DOI Open Access
Ekaterina Georgieva, Julian Ananiev,

Y. Yovchev

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8045 - 8045

Published: July 24, 2024

Oxidative stress is a major source of ROS-mediated damage to macromolecules, tissues, and the whole body. It an important marker in severe picture pathological conditions. The discovery free radicals biological systems gives "start" studying various processes related development progression many diseases. From this moment on, enrichment knowledge about participation free-radical pathogenesis cardiovascular, neurodegenerative, endocrine diseases, inflammatory conditions, infections, including COVID-19, increasing exponentially. Excessive responses abnormal reactive oxygen species (ROS) levels may disrupt mitochondrial dynamics, risk cell damage. In addition, low serum albumin changes normal physiological balance between reduced oxidized can be serious prerequisite for impaired antioxidant capacity body, worsening condition patients. This review presents interrelationship oxidative stress, inflammation, levels, which are hallmarks COVID-19.

Language: Английский

Citations

2
Development status of novel spectral imaging techniques and application to traditional Chinese medicine DOI Creative Commons
Qi Wang, Yong Zhang, Baofeng Yang

et al.

Journal of Pharmaceutical Analysis, Journal Year: 2023, Volume and Issue: 13(11), P. 1269 - 1280

Published: July 29, 2023

Traditional Chinese medicine (TCM) is a treasure of the nation, providing effective solutions to current medical requisites. Various spectral techniques are undergoing continuous development and provide new reliable means for evaluating efficacy quality TCM. Because noninvasive, convenient, sensitive, they have been widely applied in vitro vivo TCM evaluation systems. In this paper, previous achievements progress research on technologies (including fluorescence spectroscopy, photoacoustic imaging, infrared thermal laser-induced breakdown hyperspectral surface-enhanced Raman spectroscopy) discussed. The advantages disadvantages each technology also presented. Moreover, future applications imaging identify origins, components, pesticide residues elucidated. Subsequently, Identifying anticipated promote as well scientific technological explorations.

Language: Английский

Citations

6
The Impact of COVID-19 on Cellular Factors Influencing Red Blood Cell Aggregation Examined in Dextran: Possible Causes and Consequences DOI Open Access
Maciej Bosek, Tomasz Wybranowski, Marta Napiórkowska-Mastalerz

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(19), P. 14952 - 14952

Published: Oct. 6, 2023

Several studies have indicated that COVID-19 can lead to alterations in blood rheology, including an increase red cell aggregation. The precise mechanisms behind this phenomenon are not yet fully comprehended. latest findings suggest erythrocyte aggregation significantly influences microcirculation, causes the formation of clots vessels, and even damages endothelial glycocalyx, leading dysfunction. focus research lies investigating cellular factors influencing these changes discussing potential implications context pathophysiology. For purpose, erythrocytes a group 52 patients with pneumonia was examined 70 kDa Dextran solution, which eliminates influence plasma factors. Using image analysis, velocities sizes formed aggregates were investigated, determining their porosity. This study showed process patients, independent factors, leads more compact, denser, three-dimensional aggregates. These may be less likely disperse under circulatory shear stress, increasing risk thrombotic events. also suggests responsible for disorders observed long after infection, when normalized. results subsequent broad discussion presented contribute better understanding complications associated increased

Language: Английский

Citations

5
Direct Application of 3-Maleimido-PROXYL for Proving Hypoalbuminemia in Cases of SARS-CoV-2 Infection: The Potential Diagnostic Method of Determining Albumin Instability and Oxidized Protein Level in Severe COVID-19 DOI Open Access
Ekaterina Georgieva, Vasil Atanasov,

Rositsa Kostandieva

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5807 - 5807

Published: March 18, 2023

Oxidative stress and the albumin oxidized form can lead to hypoalbuminemia, which is a predisposing factor for reduced treatment effectiveness an increased mortality rate in severe COVID-19 patients. The aim of study evaluate application free radical 3-Maleimido-PROXYL SDSL-EPR spectroscopy vitro determination ox/red HSA serum samples from patients with SARS-CoV-2 infection. Venous blood was collected intubated (pO2 < 90%) positive PCR test controls. At 120th minute after incubation both groups 3-Maleimido-PROXYL, EPR measurement started. high levels radicals were determined through nitroxide TEMPOL, probably led oxidation hypoalbuminemia COVID-19. double-integrated spectra showed low degree connectivity due concentrations partially inhibit spin-label rotation, Amax values ΔH0 spectral parameters comparable those 3-Maleimido-PROXYL/DMSO. Based on obtained results, we suggest that stable be successfully used as marker

Language: Английский

Citations

4