Biology Methods and Protocols,
Journal Year:
2023,
Volume and Issue:
8(1)
Published: Jan. 1, 2023
Aquaporins
(AQPs),
transmembrane
water-conducting
channels,
have
earned
a
great
deal
of
scrutiny
for
their
critical
physiological
roles
in
healthy
and
disease
cell
states,
especially
the
biomedical
field.
Numerous
methods
been
implemented
to
elucidate
involvement
AQP-mediated
water
transport
downstream
signaling
activation
eliciting
whole
cell,
tissue,
organ
functional
responses.
To
modulate
these
responses,
other
employed
investigate
AQP
druggability.
This
review
discusses
standard
Physics of Plasmas,
Journal Year:
2023,
Volume and Issue:
30(6)
Published: June 1, 2023
In
the
cold
atmospheric
plasma
(CAP)-assisted
cancer
treatment,
increase
in
concentration
of
reactive
oxygen
species
(ROS)
and
nitrogen
(RNS)
produced
by
CAP
is
believed
to
be
related
overexpression
aquaporins
(AQPs)
on
cell
membranes.
this
paper,
using
classical
molecular
dynamics
simulations,
transmembrane
behavior
RNS
ROS
a
pure
phospholipid
bilayer
or
AQP1-phospholipid
model
was
studied.
The
research
shows
that
hydrophobic
tend
transported
through
phospholipids.
AQP1
can
significantly
improve
efficiency
hydrophilic
ROS.
Our
simulation
fills
gap
for
RNS-related
provides
guidance
precise
targeting
cells
therapy.
Biochemical and Biophysical Research Communications,
Journal Year:
2024,
Volume and Issue:
727, P. 150320 - 150320
Published: June 27, 2024
Aquaporin-0
(AQP0)
constitutes
50
%
of
the
lens
membrane
proteome
and
plays
important
roles
in
fiber
cell
adhesion,
water
permeability,
transparency.
Previous
work
has
shown
that
specific
proteins,
such
as
calmodulin
(CaM),
interact
with
AQP0
to
modulate
its
permeability;
however,
these
studies
often
used
peptides,
rather
than
full-length
protein,
probe
interactions.
Furthermore,
regions
interaction
several
known
interacting
partners,
i.e.
αA
αB-crystallins,
phakinin
(CP49)
remain
unknown.
The
purpose
this
study
was
use
crosslinking
mass
spectrometry
(XL-MS)
identify
proteins
crude
cortical
fractions
determine
protein
interaction.
Our
results
demonstrate,
for
first
time,
N-terminus
can
engage
Specific
are
elucidated
partners
including
phakinin,
α-crystallin,
connexin-46,
connexin-50.
In
addition,
two
new
vimentin
were
identified.
Biology Methods and Protocols,
Journal Year:
2023,
Volume and Issue:
8(1)
Published: Jan. 1, 2023
Aquaporins
(AQPs),
transmembrane
water-conducting
channels,
have
earned
a
great
deal
of
scrutiny
for
their
critical
physiological
roles
in
healthy
and
disease
cell
states,
especially
the
biomedical
field.
Numerous
methods
been
implemented
to
elucidate
involvement
AQP-mediated
water
transport
downstream
signaling
activation
eliciting
whole
cell,
tissue,
organ
functional
responses.
To
modulate
these
responses,
other
employed
investigate
AQP
druggability.
This
review
discusses
standard
