Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 259, P. 155388 - 155388
Published: June 5, 2024
Language: Английский
Bioactive Materials, Journal Year: 2024, Volume and Issue: 34, P. 298 - 310
Published: Jan. 3, 2024
Mesenchymal stem cell derived exosomes (MSC-Exos) demonstrate beneficial effects on wound healing via anti-inflammatory and angiogenic properties. Chitosan (CS) exhibits excellent biocompatibility accelerates cellular migration, adhesion, proliferation. The ions released from bioactive glass (BG) titanium dioxide (TiO
Language: Английский
Citations
61
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: July 5, 2024
Abstract Diabetic wounds are characterized by incomplete healing and delayed healing, resulting in a considerable global health care burden. Exosomes lipid bilayer structures secreted nearly all cells express characteristic conserved proteins parent cell-associated proteins. harbor diverse range of biologically active macromolecules small molecules that can act as messengers between different cells, triggering functional changes recipient thus endowing the ability to cure various diseases, including diabetic wounds. accelerate wound regulating cellular function, inhibiting oxidative stress damage, suppressing inflammatory response, promoting vascular regeneration, accelerating epithelial facilitating collagen remodeling, reducing scarring. from tissues or potentially possess functions varying levels promote healing. For example, mesenchymal stem cell-derived exosomes (MSC-exos) have favorable potential field due their superior stability, permeability, biocompatibility, immunomodulatory properties. Exosomes, which derived skin components, modulate inflammation regeneration key turn promotes Therefore, this review mainly emphasizes roles mechanisms sources, represented MSCs improving A deeper understanding therapeutic will yield promising candidates perspectives for management.
Language: Английский
Citations
24
International Journal of Biological Sciences, Journal Year: 2024, Volume and Issue: 20(5), P. 1778 - 1795
Published: Jan. 1, 2024
Skin tissue, composed of epidermis, dermis, and subcutaneous is the largest organ human body.It serves as a protective barrier against pathogens physical trauma plays crucial role in maintaining homeostasis.Skin diseases, such psoriasis, dermatitis, vitiligo, are prevalent can seriously impact quality patient life.Exosomes lipid bilayer vesicles derived from multiple cells with conserved biomarkers important mediators intercellular communication.Exosomes skin cells, blood, stem main types exosomes that involved modulating microenvironment.The dysregulation exosome occurrence transmission, well alterations their cargoes, complex pathogenesis inflammatory autoimmune diseases.Therefore, promising diagnostic therapeutic targets for diseases.Importantly, exogenous exosomes, or play improving environment repairing damaged tissues by carrying various specific active substances involving variety pathways.In domain clinical practice, have garnered attention prospective agents including psoriasis vitiligo.Furthermore, investigations substantiated regenerative efficacy cell-derived repair.In this review, we mainly summarize latest studies about mechanisms applications dermatology, atopic systemic lupus erythematosus, sclerosis, diabetic wound healing, hypertrophic scar keloid, aging.This will provide novel perspective diagnosis treatment dermatosis.
Language: Английский
Citations
21
Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14
Published: March 6, 2023
Introduction Full-thickness skin wound healing remains a serious undertaking for patients. While stem cell-derived exosomes have been proposed as potential therapeutic approach, the underlying mechanism of action has yet to be fully elucidated. The current study aimed investigate impact derived from human umbilical cord mesenchymal cells (hucMSC-Exosomes) on single-cell transcriptome neutrophils and macrophages in context healing. Methods Utilizing RNA sequencing, transcriptomic diversity was analyzed order predict cellular fate these immune under influence hucMSC-Exosomes identify alterations ligand-receptor interactions that may microenvironment. validity findings obtained this analysis subsequently corroborated by immunofluorescence, ELISA, qRT-PCR. Neutrophil origins were characterized based velocity profiles. Results expression RETNLG SLC2A3 associated with migrating neutrophils, while BCL2A1B linked proliferating neutrophils. group exhibited significantly higher levels M1 (215 vs 76, p < 0.00001), M2 (1231 670, (930 157, 0.00001) when compared control group. Additionally, it observed elicit differentiation trajectories towards more anti-inflammatory phenotypes, concomitant changes interactions, thereby facilitating Discussion This revealed heterogeneity repair following interventions, providing deeper understanding responses hucMSC-Exosomes, rising target intervention.
Language: Английский
Citations
24
Extracellular Vesicle, Journal Year: 2024, Volume and Issue: 3, P. 100034 - 100034
Published: March 2, 2024
Mesenchymal stem cells (MSCs) have been studied for decades as candidates cellular therapy, and their secretome, including secreted extracellular vesicles (EVs), has identified to contribute significantly regenerative reparative functions. Emerging evidence suggested that MSC-EVs alone, could be used therapeutics emulate the biological function of MSCs. However, just with MSCs, shown vary in composition, depending on tissue source MSCs well protocols employed culturing obtaining EVs. Therefore, importance careful choice cell sources culture environments is receiving increasing attention. Many factors therapeutic potential MSC-EVs, tissue, isolation technique, conditions. This review illustrates molecular landscape EVs derived from different types MSC along strategies. A thorough analysis publicly available omic datasets was performed advance precision understanding unique source-dependent characteristics. The tissue-specific protein miRNA-driven Reactome ontology reveal distinct patterns top pathways across adipose, bone marrow, umbilical MSC-EVs. Moreover, a meta-analysis assisted by an AI technique analyze published literature, providing insights into translation based tissues.
Language: Английский
Citations
13
Giant, Journal Year: 2024, Volume and Issue: 18, P. 100251 - 100251
Published: March 6, 2024
The treatment of diabetic wounds is a major challenge faced by the medical system, and there growing interest in developing innovative therapies to accelerate wound healing. Regenerative medicine with cells has shown promising potential skin repair, regenerative properties primarily attributed paracrine effects secreted products, including exosomes. Compared cell-based approaches, using exosomes as cell-free therapy for chronic several advantages. Exosomes can regulate intercellular communication releasing their contents, mRNA, miRNA, lipids, proteins, which further promote are well explored biomedical application owing advantages such biocompatibility low immunogenicity. However, common method exosome administration through injection, but due rapid clearance rate body, maintaining necessary therapeutic concentration around challenging. Therefore, it develop new biocompatible scaffold carrier extracellular vesicles, allowing them sustain at non-healing sites continuously Engineered kinds modified internal treated molecules, surface decoration or delivered engineered platform. In addition, some researchers have processed exosomes, known decoration, delivery platforms. regular greater promoting this review, we summarize molecular mechanisms from different sources varying modifications Advantages limitations repair were also discussed. Finally, highlight challenges future development directions translating our knowledge into clinical practice.
Language: Английский
Citations
11
International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 3555 - 3575
Published: April 1, 2024
Background: Neutrophils rapidly accumulate in large numbers at sites of tissue damage, exhibiting not only their well-known bactericidal capabilities but also playing crucial roles angiogenesis and repair. While exosomes derived from human umbilical cord mesenchymal stem cells (HucMSC-Exo) have emerged as a promising therapeutic tool, exact mechanisms action remain partly elusive. We hypothesize that HucMSC-Exo treatment may modulate neutrophil phenotypes, thereby significantly influencing wound healing outcomes. Methods: were isolated via ultracentrifugation subsequently administered through subcutaneous injection into full-thickness cutaneous wounds mice. To determine the impact host neutrophils on effects skin injuries, strategies including depletion adoptive transfer employed. Flow cytometry was used to evaluate proportion N2 subtype both normal diabetic wounds, effect this assessed. Furthermore, mitochondrial metabolic reprogramming driven by during polarization investigated JC1 staining, ATP quantification, fatty acid uptake assays, assessment FAO-related genes ( Cpt1b, Acadm, Acadl ). Results: Depleting strikingly dampened prohealing injury, while bone marrow rescued process. During process, some expressed markers, contrast, exhibited reduced expression markers. After with HucMSC-Exo, most increased phosphorylation STAT6, leading thus acquired an phenotype. These neutrophils, polarized boosted release proangiogenic factors, particularly BV8, myeloid cell-derived factor, induced favoring restoration. Conclusion: This research uniquely demonstrates identification injury shows could skew toward phenotype, enhancing our insight how react HucMSC-Exo. Keywords: phenotype switching, healing, restoration
Language: Английский
Citations
9
International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 1543 - 1560
Published: Feb. 1, 2025
Abstract: Tissue injury repair is a multifaceted and dynamic process characterized by complex interactions among various immune cells, with M2 macrophages assuming crucial role. Exosomes derived from M2-type (M2-Exos) significantly influence the through intercellular communication mediated enriched microRNAs (miRNAs). This review aims to elucidate biological processes underlying exosome formation, synthesis function of miRNAs, diverse methodologies employed for extraction. Furthermore, we provide comprehensive summary established multifarious functions mechanisms M2-Exos miRNAs in tissue across different systems, while also exploring their potential applications disease prevention, diagnosis, clinical practice. Despite challenges encountered, therapeutic use contexts appears promising, prompting research efforts focus on improving efficiency extraction application, as well ensuring safety utilization. Keywords: macrophages, exosomes, repair, drug delivery engineered
Language: Английский
Citations
1
Molecular and Cellular Biochemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 11, 2025
Language: Английский
Citations
1
International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 2707 - 2720
Published: May 1, 2023
Abstract: Nowadays, refractory diabetic wounds cause a worldwide medical burden. Mesenchymal stem cells derived exosomes (MSC-Exos) show promise as solid alternative to existing therapeutics in the latest researches, since MSC-Exos share similar biologic activity but less immunogenicity when compared with MSCs. To facilitate further understanding and application, it is essential summarize current progress limitations of treatment wounds. In this review, we introduce effects different on according their origins contents discuss specific experimental conditions, target wound cells/pathways, mechanisms. addition, paper focuses combination biomaterials, which improves efficacy utilization therapy. Together, exosome therapy has high clinical value application prospects, both its role while novel drugs or molecules loaded into carriers targeting will be development trends. Keywords: wound, mesenchymal cell, exosome, biomaterial
Language: Английский
Citations
21