Human umbilical cord mesenchymal stem cells derived extracellular vesicles alleviate salpingitis by promoting M1–to–M2 transformation

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 16, 2023

Background: With an increasing number of patients experiencing infertility due to chronic salpingitis after Chlamydia trachomatis (CT) infection, there is unmet need for tissue repair or regeneration therapies. Treatment with human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hucMSC-EV) provides attractive cell-free therapeutic approach. Methods: In this study, we investigated the alleviating effect hucMSC-EV on tubal inflammatory caused by CT using in vivo animal experiments. Furthermore, examined inducing macrophage polarization explore molecular mechanism. Results: Our results showed that infection was significantly alleviated treatment group compared control group. Further mechanistic experiments application induced from M1 M2 type via NF-κB signaling pathway, improved local microenvironment fallopian tubes and inhibited tube inflammation. Conclusion: We conclude approach represents a promising avenue ameliorate salpingitis.

Language: Английский

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Exosomal microRNAs from Mesenchymal Stem Cells: Novel Therapeutic Effect in Wound Healing

Tissue Engineering and Regenerative Medicine, Journal Year: 2023, Volume and Issue: 20(5), P. 647 - 660

Published: May 2, 2023

Language: Английский

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IFN‐γ enhances the therapeutic efficacy of MSCs‐derived exosome via miR‐126‐3p in diabetic wound healing by targeting SPRED1

Journal of Diabetes, Journal Year: 2023, Volume and Issue: 16(1)

Published: Aug. 30, 2023

Abstract Background and Aims The traditional treatment of diabetic wounds is unsatisfactory. Exosomes isolated from bone marrow mesenchymal stem cells (BMSCs) promote the healing wounds. However, whether exosomes secreted by interferon (IFN)‐γ‐pretreated BMSCs have an enhanced therapeutic effect on wound relevant mechanisms remain unclear. Methods In this study, we corresponding supernatants with (IExos) or without IFN‐γ (NExos). Human umbilical vein endothelial (HUVECs) were used to investigate proliferation, migration, tube formation under different treatments in vitro. Diabetic mice induced intraperitoneal administration streptozotocin, a circular full‐thickness dermal defect was then made back each mouse, followed multisite subcutaneous injection phosphate buffered saline exosomes. Hematoxylin–eosin (H&E) staining, Masson's trichrome histological analysis performed assess speed quality healing. Results NExos accelerated promoting angiogenesis vivo vitro, IExos exhibited superior efficiency. MicroRNA (miR)‐126‐3p significantly increased IExos, exosomal miR‐126‐3p promoted via its transfer HUVECs. regulates SPRED1 directly targeting 3′‐UTR. Mechanistically, IFN‐γ‐pretreated miR‐126‐3p‐enriched exosomes, which function HUVECs SPRED1/Ras/Erk pathway. Conclusion Exosomal higher efficacy than axis.

Language: Английский

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N6-Methyladenosine Modification of lncCCKAR-5 Regulates Autophagy in Human Umbilical Cord Mesenchymal Stem Cells by Destabilizing LMNA and Inhibits Diabetic Wound Healing

Journal of Investigative Dermatology, Journal Year: 2024, Volume and Issue: 144(5), P. 1148 - 1160.e15

Published: Jan. 17, 2024

Language: Английский

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The science of exosomes: Understanding their formation, capture, and role in cellular communication

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 259, P. 155388 - 155388

Published: June 5, 2024

Language: Английский

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