From bench to bedside: the research status and application opportunity of extracellular vesicles and their engineering strategies in the treatment of skin defects DOI Creative Commons
Lei Cui,

Yingfang Song,

Zhipeng Hou

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: May 25, 2025

Engineered extracellular vesicles (EVs), which are EVs modified to enhance certain biological properties, offer a promising therapeutic strategy for the treatment of skin defects. Conventional nanomaterials often encounter clinical translation challenges due potential toxicity and limited targeting. EVs, utilizing inherent biocompatibility effective physiological barrier traversal, can ameliorate limitations conventional EV therapies some extent, including detection, isolation, purification, validation. Recent advances in engineering, such as genetic modification production cells control cargo, surface engineering targeted delivery, pre-treatment parental optimize bioactivity, have improved efficacy laboratory studies through enhanced targeting, prolonged retention time, increased yield. Many suggested ability engineered treat variety defects, diabetic wounds, burns, hypertrophic scars, providing avenue their this area. This paper reviews regeneration, highlighting role promoting cell migration angiogenesis, modulating inflammation reducing scar formation during wound healing. In addition, given investment rapidly evolving field growing trial activity, review also explores recent global provides an outlook on future application opportunities

Language: Английский

Engineering exosome-based biomimetic nanovehicles for wound healing DOI Creative Commons

Alireza Joorabloo,

Tianqing Liu

Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 356, P. 463 - 480

Published: March 15, 2023

Complexity and difficulties in wound management are pressing concerns that affect patients' quality of life may result tissue infection, necrosis, loss local systemic functions. Hence, novel approaches to accelerate healing being actively explored over the last decade. Exosomes as important mediators intercellular communications promising natural nanocarriers due their biocompatibility, low immunogenicity, drug loading targeting capacities, innate stability. More importantly, exosomes developed a versatile pharmaceutical engineering platform for repair. This review provides an overview biological physiological functions derived from variety origins during phases, strategies exosomal engineering, therapeutic applications skin regeneration.

Language: Английский

Citations

48
Recent advances in the use of extracellular vesicles from adipose-derived stem cells for regenerative medical therapeutics DOI Creative Commons

Song Yang,

Yiran Sun,

Chenchen Yan

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: June 6, 2024

Abstract Adipose-derived stem cells (ADSCs) are a subset of mesenchymal (MSCs) isolated from adipose tissue. They possess remarkable properties, including multipotency, self-renewal, and easy clinical availability. ADSCs also capable promoting tissue regeneration through the secretion various cytokines, factors, extracellular vesicles (EVs). ADSC-derived EVs (ADSC-EVs) act as intercellular signaling mediators that encapsulate range biomolecules. These have been found to mediate therapeutic activities donor by proliferation migration effector cells, facilitating angiogenesis, modulating immunity, performing other specific functions in different tissues. Compared themselves, ADSC-EVs offer advantages such fewer safety concerns more convenient transportation storage for application. As result, these received significant attention cell-free agents with potential future application regenerative medicine. In this review, we focus on recent research progress regarding medical use across conditions, wound healing, chronic limb ischemia, myocardial infarction, diabetic nephropathy, fat graft survival, bone regeneration, cartilage tendinopathy tendon peripheral nerve acute lung injury, among others. We discuss underlying mechanisms responsible inducing effects. believe deciphering biological effects, associated will provide foundation developing novel approach Graphical

Language: Английский

Citations

25
Small extracellular vesicles: Yields, functionalization and applications in diabetic wound management DOI Creative Commons
Xi Liu, Wei Qian, Zijie Sun

et al.

Deleted Journal, Journal Year: 2023, Volume and Issue: 1(4)

Published: Oct. 1, 2023

Abstract Diabetic wounds have imposed a significant burden on both patients and society with prolonged healing processes hindered by dysfunctional skin repair cells. Small extracellular vesicles (sEVs), as the important media for intercellular communications, show promising therapeutic potential in treating diabetic restoring cellular functions. However, low yields limited bio‐function of sEVs greatly challenge their large‐scale clinical use. Here, we briefly overview biogenesis uptake sEVs, emphasize current advances improving optimizing function engineering approaches, summarize applications engineered wound treatment. Furthermore, undissolved issues during transformation are also discussed. This critical review aims to provide meaningful guidance future management wounds.

Language: Английский

Citations

31
Mechanisms and applications of adipose-derived stem cell-extracellular vesicles in the inflammation of wound healing DOI Creative Commons
Qingyi Jia,

Hanxing Zhao,

Yixi Wang

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: July 14, 2023

Wound healing is a sophisticated process consisting of serial phases with overlaps, including hemostasis, inflammation, proliferation, and remodeling. The inflammation response an early that plays crucial role in eliminating microbes clearing damaged cell debris. However, some pathological circumstances, such as diabetes mellitus, ischemia, trauma, deep burn, etc., abnormal can cause impaired wound healing. Adipose-derived stem cells (ADSCs) belong to the mesenchymal (MSC) family exhibit prospective applications tissue regeneration dermatological repairs. ADSC-secreted extracellular vesicles (ADSC-EVs) mimic functions ADSCs without concerns survival, immune response, or ethical issues. Studies have revealed ADSC-EVs inhibit responses accelerate through various mechanisms. Moreover, studies explored modifications cargo components enhance their therapeutic efficacy. Given increasing focusing on potential healing, how they interfere different this has been investigated pieces. In review, we summarized all up-to-date evidence map clearer picture underlying mechanisms response. aiming at were also reviewed provide strategies for future investigators.

Language: Английский

Citations

24
Exosome: From biology to drug delivery DOI

Himakshi Baruah,

Anupam Sarma,

Debojeet Basak

et al.

Drug Delivery and Translational Research, Journal Year: 2024, Volume and Issue: 14(6), P. 1480 - 1516

Published: Jan. 22, 2024

Language: Английский

Citations

15
The mechanism of selenium regulating the permeability of vascular endothelial cells through selenoprotein O DOI Creative Commons
Jiawei Wu,

Yanhe Zhang,

Tianjing Liu

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 70, P. 103063 - 103063

Published: Feb. 2, 2024

Vascular diseases, a leading cause of death in human, are strongly associated with pathological damage to blood vessels. The selenoprotein (Sel) have been reported play important roles vascular disease. However, the role SelO disease has not conclusively investigated. present experiment was investigate regulatory mechanism effect on permeability endothelial. H.E staining, FITC-Dextran Dil-AC-LDL staining and FITC-WGA showed that structure damaged, intercellular junctions were disrupted selenium (Se)-deficient. Immunohistochemistry, qPCR Western blot revealed decreased expression adhesion plaque proteins vinculin, talin paxillin, connectivity effector molecules connexin, claudin-1 E-cadherin increased JAM-A N-cadherin, as well ZO-1 signaling pathways ZO-1, Rock, rhoGEF, cingulin MLC-2. In screening 24 Sel mice, most pronounced changes tissues, possible association between junction effectors determined using IBM SPSS Statistics 25. Silencing SelO, endothelial adverse effects present. relationship determined. results Se deficiency lead tissue by decreasing expression, suggesting for regulating permeability.

Language: Английский

Citations

15
Opportunities and challenges of engineered exosomes for diabetic wound healing DOI Creative Commons

Lin Shi,

Song Dawei,

Cong Meng

et al.

Giant, Journal Year: 2024, Volume and Issue: 18, P. 100251 - 100251

Published: March 6, 2024

The treatment of diabetic wounds is a major challenge faced by the medical system, and there growing interest in developing innovative therapies to accelerate wound healing. Regenerative medicine with cells has shown promising potential skin repair, regenerative properties primarily attributed paracrine effects secreted products, including exosomes. Compared cell-based approaches, using exosomes as cell-free therapy for chronic several advantages. Exosomes can regulate intercellular communication releasing their contents, mRNA, miRNA, lipids, proteins, which further promote are well explored biomedical application owing advantages such biocompatibility low immunogenicity. However, common method exosome administration through injection, but due rapid clearance rate body, maintaining necessary therapeutic concentration around challenging. Therefore, it develop new biocompatible scaffold carrier extracellular vesicles, allowing them sustain at non-healing sites continuously Engineered kinds modified internal treated molecules, surface decoration or delivered engineered platform. In addition, some researchers have processed exosomes, known decoration, delivery platforms. regular greater promoting this review, we summarize molecular mechanisms from different sources varying modifications Advantages limitations repair were also discussed. Finally, highlight challenges future development directions translating our knowledge into clinical practice.

Language: Английский

Citations

12
Fabrication, characterization, and application of gelatin/alginate-based hydrogels incorporating selenium-doped deferoxamine-derived carbon quantum dots: In vitro and in vivo studies DOI
Mohammad Amin Amini, Iraj Khodadadi, Heidar Tavilani

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140569 - 140569

Published: Feb. 1, 2025

Language: Английский

Citations

1
A thermally stable bioactive chitosan scaffold with pH-responsive exosome adsorption and release function promotes wound healing DOI Creative Commons

Jifang Yuan,

Meirong Li, Xiaofeng He

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141552 - 141552

Published: Feb. 1, 2025

Language: Английский

Citations

1
Recent advances in extracellular vesicle engineering and its applications to regenerative medicine DOI Creative Commons
Won‐Kyu Rhim, Jun Yong Kim, Seung Yeon Lee

et al.

Biomaterials Research, Journal Year: 2023, Volume and Issue: 27(1)

Published: Feb. 9, 2023

Extracellular vesicles (EVs) are nanosized particles that released from cells and reflect the characteristics of mother cell. Recently, EVs have been used in several types studies across many different fields. In field EV research, multiple cell culture isolation techniques highlighted importance. Various strategies, including exclusive component media, three-dimensional (3D) cultures, hypoxic conditions, proposed for to control function EVs. Ultracentrifugation, ultrafiltration, precipitation, tangential flow filtration (TFF) utilized isolation. Although isolated their own functionalities, researchers trying functionalize by applying various engineering approaches. Gene editing, exogenous, endogenous, hybridization methods four well-known functionalization strategies. engineered through these processes has applied regenerative medicine, kidney diseases, osteoarthritis, rheumatoid arthritis, nervous system-related others. this review, it was focused on approaches applications medicine.

Language: Английский

Citations

22