Assessment of the excretion of oxidative stress biomarkers and anabolic steroids based on sewage: A case study of college students and the general population
Zhihan Gao,
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Sun Hui,
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Yufei Xie
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et al.
The Science of The Total Environment,
Journal Year:
2023,
Volume and Issue:
878, P. 163079 - 163079
Published: March 27, 2023
Language: Английский
Preliminary evaluation of the antiglycoxidant activity of verapamil using various in vitro and in silico biochemical/biophysical methods
Frontiers in Pharmacology,
Journal Year:
2023,
Volume and Issue:
14
Published: Nov. 28, 2023
Introduction:
Glycoxidative
stress
is
essential
for
linking
glucose
disturbances
and
cardiovascular
diseases.
Unfortunately,
contemporary
antidiabetic
drugs
do
not
have
an
antiglycative
effect
but
only
lower
blood
levels.
Therefore,
there
intense
search
substances
that
could
inhibit
protein
glycation
prevent
diabetic
complications.
A
potential
antioxidant
activity
has
been
demonstrated
with
verapamil,
a
phenylalkylamine
derivative
belonging
to
selective
calcium
channel
blockers.
Verapamil
well-established
position
in
cardiology
due
its
wide
range
of
indications
good
safety
profile.
Nevertheless,
the
verapamil
still
unclear.
We
are
first
comprehensively
evaluate
verapamil’s
on
glycoxidation
using
various
vitro
silico
models.
Methods:
Bovine
serum
albumin
(BSA)
was
used
assess
rate
inhibition
by
verapamil.
As
glycating
factors,
sugars
(glucose,
fructose,
ribose)
aldehyde
(glyoxal)
were
used.
Chloramine
T
as
oxidizing
agent.
Aminoguanidine
(protein
inhibitor)
Trolox
(antioxidant)
control
substances.
The
biomarkers
oxidation
(total
thiols,
carbonyls,
advanced
products),
(Amadori
products,
β-amyloid,
end
products
[AGEs]),
(tryptophan,
kynurenine,
N-formylkynurenine,
dityrosine)
evaluated
colorimetric
fluorimetric
methods.
mechanism
assessed
docking
study
interaction
BSA,
glycosidases,
seventeen
AGE
pathway
proteins.
Results:
In
all
models,
glycation,
oxidation,
significantly
ameliorated
under
influence
comparable
potent
antiglycating
agents
antioxidants.
molecular
simulations
showed
bound
preferentially
amino
acids
prone
glycoxidative
damage
out
α-glucosidase’s
active
center.
Among
proteins,
best
docked
Janus
kinase
2
(JAK2)
nuclear
factor-κB
(NF-κB).
Discussion:
results
our
confirm
antiglycoxidant
properties
drug’s
action
recognized
protecting
against
oxidative
modifications.
may
be
particularly
helpful
patients
disease
concomitant
diabetes.
Studies
animal
models
humans
needed
antiglycative/antidiabetic
activity.
Language: Английский
Activity of exoglycosidases in blood, urine, cerebrospinal fluid, and vitreous humor in individuals who died from ethyl alcohol poisoning
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Sept. 30, 2024
Language: Английский
Impact of Substance Use Disorder on Tryptophan Metabolism Through the Kynurenine Pathway: A Narrative Review
Metabolites,
Journal Year:
2024,
Volume and Issue:
14(11), P. 611 - 611
Published: Nov. 10, 2024
:
Substance
use
disorder
is
a
crisis
impacting
many
people
in
the
United
States.
This
review
aimed
to
identify
effect
addictive
substances
have
on
kynurenine
pathway.
Tryptophan
an
essential
amino
acid
metabolized
by
serotonin
and
pathways.
The
metabolites
of
these
pathways
play
role
biological
reward
system.
Addictive
been
shown
cause
imbalances
ratios
metabolites.
With
current
treatment
therapeutic
options
being
suboptimal,
identifying
biochemical
mechanisms
that
are
impacted
during
can
provide
alternative
for
or
drug
discovery.
Language: Английский
Total Antioxidant and Oxidative Status as Potential Biomarkers of Alcohol Overdose
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
26(1), P. 82 - 82
Published: Dec. 25, 2024
Serious
alcohol-associated
hazards
underscore
the
need
to
develop
new
biomarkers
reflecting
biological
changes
caused
by
chronic
alcohol
use
and
predicting
risk
of
alcohol-related
death.
Oxidative
stress
is
one
mechanism
toxicity.
The
blood
urine
redox
status
(total
antioxidant
capacity
[TAC],
total
oxidative
[TOS],
index
[OSI])
was
assessed
in
105
people
who
died
a
sudden
death
(controls),
47
overdose,
102
with
dependency.
TAC
TOS
were
determined
utilizing
colorimetric
method.
Non-parametric
tests
used
for
statistical
analysis.
Blood
levels
significantly
elevated
individuals
both
dependency
poisoning
compared
controls.
study
groups
control
group,
higher
patients
group
poisoning.
highly
correlated
content.
Receiver
operating
characteristic
(ROC)
analysis
showed
that
effectively
discriminated
between
high
sensitivity
specificity.
Our
confirmed
impaired
homeostasis
alcoholism
indicated
utility
TAC,
TOS,
OSI
as
exposure.
Language: Английский