Efficient and feasible biocatalysts: Strategies for enzyme improvement. A review
Samah Hashim Albayati,
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Nima Ghahremani Nezhad,
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Abdulrahman M Taki
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et al.
International Journal of Biological Macromolecules,
Journal Year:
2024,
Volume and Issue:
276, P. 133978 - 133978
Published: July 20, 2024
Language: Английский
A comprehensive review of lipase-catalyzed acidolysis as a method for producing structured glycerides
International Journal of Biological Macromolecules,
Journal Year:
2025,
Volume and Issue:
unknown, P. 142878 - 142878
Published: April 1, 2025
Language: Английский
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support
Biotechnology Progress,
Journal Year:
2023,
Volume and Issue:
40(1)
Published: Oct. 12, 2023
In
this
article,
we
have
analyzed
the
interactions
between
enzyme
crowding
on
a
given
support
and
its
chemical
modification
(ethylenediamine
via
carbodiimide
route
picryl
sulfonic
(TNBS)
of
primary
amino
groups)
activity
stability.
Lipase
from
Thermomyces
lanuginosus
(TLL)
lipase
B
Candida
antarctica
(CALB)
were
immobilized
octyl-agarose
beads
at
two
very
different
loadings,
one
them
exceeding
capacity
support,
well
under
capacity.
Chemical
modifications
highly
loaded
lowly
biocatalysts
gave
results
in
terms
stability,
which
could
increase
or
decrease
depending
loading.
For
example,
both
increased
their
after
while
effect
was
opposite
for
biocatalysts.
Additionally,
with
TNBS
CALB
biocatalyst
stability
activity.
Language: Английский
The nature of the buffer alters the effects of the chemical modification on the stability of immobilized lipases
Process Biochemistry,
Journal Year:
2023,
Volume and Issue:
133, P. 20 - 27
Published: Aug. 9, 2023
The
objective
of
this
paper
was
to
analyze
whether
an
interaction
between
the
effects
buffer
nature
and
chemical
modification
on
enzyme
stability
exists.
For
this,
lipase
B
from
Candida
antarctica
(CALB)
Thermomyces
lanuginosus
(TLL)
were
immobilized
octyl
agarose
beads
modified
with
picryl
sulfonic
acid
(TNBS)
ethylenediamine
via
carbodiimide
route.
obtained
biocatalysts
them
inactivated
in
different
buffers
(2-amino-2-(hydroxymethyl)propane-1,3-diol
(Tris)-HCl,
N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic
acid)
(HEPES)
or
phosphate).
A
significant
found
surfaces
their
stabilities
these
buffers.
While
phosphate
where
lowest
for
both
unmodified
enzymes,
differences
become
much
smaller
after
some
modifications.
In
many
instances,
improves
when
using
a
negative
other
one
(e.g.,
TNBS
TLL
positive
Tris-HCl
buffers,
amination
CALB
decreased
its
HEPES
while
it
almost
had
no
effect
phosphate).Thus,
clear
co-interactions
established.
Language: Английский
Mineralization of Lipase from Thermomyces lanuginosus Immobilized on Methacrylate Beads Bearing Octadecyl Groups to Improve Enzyme Features
Catalysts,
Journal Year:
2022,
Volume and Issue:
12(12), P. 1552 - 1552
Published: Dec. 1, 2022
Lipase
from
Thermomyces
lanuginosus
(TLL)
has
been
immobilized
on
Purolite
Lifetech®
ECR8806F
(viz.
methacrylate
macroporous
resin
containing
octadecyl
groups,
designated
as
C18-TLL),
and
the
enzyme
performance
compared
to
that
of
octyl-agarose,
agarose
C8-TLL.
The
hydrolytic
activity
versus
p-nitrophenol
butyrate
decreased
significantly,
a
lower
extent
S-methyl
mandelate
(more
than
twofold),
while
triacetin
R-methyl
mandelate,
was
higher
for
biocatalyst
prepared
using
C18
(up
almost
five-fold).
Regarding
stability,
C18-TLL
significantly
more
stable
Next,
biocatalysts
were
mineralized
zinc,
copper
or
cobalt
phosphates.
Mineralization
increased
this
very
S-isomer,
effects
C8-TLL
diverse
(hydrolytic
increase
decrease
dependent
metal
substrate).
zinc
salt
treatment
stability
both
biocatalysts,
but
with
impact
agarose-C8-TLL.
On
contrary,
treatments
intensively
C18-TLL.
results
show
even
enzymes
following
same
strategy,
differences
in
conformation
cause
mineralization
have
activity,
specificity.
Language: Английский
Fe3O4@silica-thermolysin: A robust, advantageous, and reusable microbial nanobiocatalyst for proteolysis and milk-clotting
Vitor A. Ungaro,
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João Pedro Alves Fairbanks,
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Liane M. Rossi
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et al.
International Journal of Biological Macromolecules,
Journal Year:
2024,
Volume and Issue:
278, P. 134503 - 134503
Published: Aug. 5, 2024
Language: Английский
Short-Term Stability of Electrochemical Properties of Layer-by-Layer Coated Heterogeneous Ion Exchange Membranes
Membranes,
Journal Year:
2022,
Volume and Issue:
13(1), P. 45 - 45
Published: Dec. 29, 2022
Layer-by-layer
adsorption
allows
the
creation
of
versatile
functional
coatings
for
ion
exchange
membranes,
but
stability
coating
and
resulting
properties
modified
membranes
in
their
operation
is
a
frequently
asked
question.
This
paper
examines
changes
voltammetric
curves
layer-by-layer
coated
cation
pH-metry
desalination
chamber
with
studied
membrane
an
auxiliary
anion
after
short-term
tests,
including
over-limiting
current
modes.
The
practical
did
not
affect
curves,
enhanced
generation
H+
OH−
ions
system
polyethylenimine
Ca2+
containing
solution.
It
shown
that
distinction
between
different
polyamines
persists
during
that,
case
polyethylenimine,
there
additional
zone
growth
potential
drop
stronger
as
indicated
by
pH-metry.
Language: Английский