Acta Tropica, Journal Year: 2024, Volume and Issue: 254, P. 107182 - 107182
Published: March 11, 2024
Language: Английский
Bioengineering, Journal Year: 2024, Volume and Issue: 11(11), P. 1090 - 1090
Published: Oct. 30, 2024
Cardiovascular diseases (CVDs) are leading causes of morbidity and mortality globally, characterized by complications such as heart failure, atherosclerosis, coronary artery disease. The vascular endothelium, forming the inner lining blood vessels, plays a pivotal role in maintaining homeostasis. dysfunction endothelial cells contributes significantly to progression CVDs, particularly through impaired cellular communication paracrine signaling with other cell types, smooth muscle macrophages. In recent years, co-culture systems have emerged advanced vitro models for investigating these interactions mimicking pathological environment CVDs. This review provides an in-depth analysis that explore development diseases. It summarizes advancements multicellular models, their physiological therapeutic relevance, insights they provide into molecular mechanisms underlying Additionally, we evaluate advantages limitations offering perspectives on how can be utilized novel strategies drug testing cardiovascular research.
Language: Английский
Citations
3
Tissue Engineering Part B Reviews, Journal Year: 2024, Volume and Issue: 30(4), P. 462 - 476
Published: Jan. 24, 2024
Exosomes are nanosized extracellular vesicles (EVs) that participate in intercellular communication through surface proteins and the delivery of internal cargo. The exosomes have gained attention for their potential as disease biomarkers therapeutic agents. ability has been verified by copious previous studies. Effective methods extensive clinical applications being researched exosome-based regenerative therapies, including application 3D cultures to enhance exosome production secretion, which can resolve limited secretion from parent cells. Cell culture emerged a crucial approach biomedical research because its many benefits. Both well-established continuous cell lines primary continue be invaluable basic application. Previous studies shown three-dimensional cultured (3D-Exo) improve properties yields compared with traditional systems. Since majority focused on derived mesenchymal stem cells (MSC-Exo), this review will also concentrate MSC-Exo. In review, we summarize advantages 3D-Exo introduce system isolation, providing scientific strategies diagnosis, treatment, prognosis wide variety diseases. introduced more comprehensive perspective provided an overview current applications. This article offered readers insights into status, development trends, key issues research. Furthermore, pointed out existing problems limitations while suggesting directions improvement further
Language: Английский
Citations
2
Animals, Journal Year: 2024, Volume and Issue: 14(5), P. 803 - 803
Published: March 5, 2024
In vitro gamete derivation has been proposed as an interesting strategy for treatment of infertility, improvement genetic traits, and conservation endangered animals. Spermatogonial stem cells (SSCs) are primary candidates in derivation; however, recently, mesenchymal (MSCs) have also germ cell (GCs) differentiation mainly due to their transdifferentiating capacity. The objective the present study was compare potential GC bovine peripheral blood-derived MSCs (PB-MSCs) SSCs under effect conditioned medium (CM) derived from Sertoli (SCs/CM). Samples were collected every 7 days 21 analyzed pluripotent, GC, MSC marker expression. absence OCT4 increased (p < 0.05) expression NANOG seems play a role SSC differentiation, whereas may be required PB-MSC into GCs. cultured with SCs/CM PIWIL2 DAZL, while PB-MSCs same condition only DAZL. Overall, patterns markers suggest that activate different signaling pathways after exposure during
Language: Английский
Citations
2
Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(7), P. 4469 - 4481
Published: June 15, 2024
A facile method was proposed for preparing controllable multicompartment gel microcarriers using an aqueous two-phase emulsion system. By leveraging the density difference between upper polyethylene glycol solution and lower dextran-calcium chloride (CaCl2) in collection high viscosity of solution, fusion core–shell droplets made by coextrusion devices achieved at water/water (w/w) interface to fabricate with separated core compartments. adjusting sodium alginate concentration, collected composition, number fused liquid droplets, pore size, shape, compartments could be controlled. Caco-2 HepG2 cells were encapsulated different establish gut–liver coculture models, exhibiting higher viability proliferation compared monoculture models. Notably, significant differences cytokine expression functional proteins observed This provides new possibilities complex three-dimensional materials.
Language: Английский
Citations
2
Acta Tropica, Journal Year: 2024, Volume and Issue: 254, P. 107182 - 107182
Published: March 11, 2024
Language: Английский
Citations
1