Hypoxia induced exosomal Circ-ZNF609 promotes pre-metastatic niche formation and cancer progression via miR-150-5p/VEGFA and HuR/ZO-1 axes in esophageal squamous cell carcinoma

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: March 12, 2024

Abstract Exosomes derived from cancer are regarded as significant mediators of cancer-host crosstalk. Hypoxia, on the other hand, is one essential characteristics solid tumors. This research set out to discover how circulating exosomes hypoxic esophageal squamous cell carcinoma (ESCC) contribute formation metastatic niches and distant metastasis. First, we noticed that human umbilical vein endothelial cells (HUVECs) had their tight connections disrupted expression proteins involved in angiogenesis boosted by ESCC exosomes. Hypoxia significantly induced Circ-ZNF609 ESCC, which was then internalized HUVECs, determined circular RNA screening. High HUVECs facilitated vascular permeability, thereby promoting pre-metastatic niche formation, enhancing metastasis vitro vivo. Exosomal activated growth factor A (VEGFA) mechanistically sponging miR-150-5p. also interacted with HuR inhibited binding ZO-1, Claudin-1, Occludin mRNAs, reducing translation. Collectively, our findings identified an function for exosomal cells, suggesting potential therapeutic value patients.

Language: Английский

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MiR-223-3p in Cancer Development and Cancer Drug Resistance: Same Coin, Different Faces

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8191 - 8191

Published: July 26, 2024

MicroRNAs (miRNAs) are mighty post-transcriptional regulators in cell physiology and pathophysiology. In this review, we focus on the role of miR-223-3p (henceforth miR-223) various cancer types. MiR-223 has established roles hematopoiesis, inflammation, most cancers, where it can act as either an oncogenic or oncosuppressive miRNA, depending specific molecular landscapes. also been linked to sensitivity resistance cells treatments a context-dependent way. Through detailed highlight that for some cancers (i.e., breast, non-small lung carcinoma, glioblastoma), miR-223 is consistently reported literature, while others colorectal, ovarian, pancreatic acute lymphocytic leukemia), prevails. prostate other hematological malignancies, although frequently described, there less consensus. Intriguingly, NLRP3 FBXW7 identified targets when miRNA acts oncosuppressor oncogene, respectively, different cancers. Our review describes was increased biological fluids their extracellular vesicles analyzed, compared healthy lower-risk conditions, confirming potential application diagnostic prognostic biomarker clinic.

Language: Английский

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The Glioblastoma CircularRNAome

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(19), P. 14545 - 14545

Published: Sept. 26, 2023

Glioblastoma remains one of the most aggressive cancers brain, warranting new methods for early diagnosis and more efficient treatment options. Circular RNAs (circRNAs) are rather entities with increased stability compared to their linear counterparts that interact proteins act as microRNA sponges, among other functions. Herein, we provide a critical overview recently described glioblastoma-related circRNAs in literature, focusing on roles glioblastoma cancer cell proliferation, survival, migration, invasion metastasis, metabolic reprogramming, therapeutic resistance. The main regulating processes due regulatory essential oncogenic pathways, including MAPK, PI3K/AKT/mTOR, Wnt, which influenced by various circRNAs. present work pictures wide implication glioblastoma, thus highlighting potential future biomarkers targets/agents.

Language: Английский

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A Circular RNA Derived from the Pumilio 1 Gene Could Regulate PTEN in Human Cumulus Cells

Genes, Journal Year: 2024, Volume and Issue: 15(1), P. 124 - 124

Published: Jan. 19, 2024

CircRNAs are a class of non-coding RNAs able to regulate gene expression at multiple levels. Their involvement in physiological processes, as well their altered regulation different human diseases, both tumoral and non-tumoral, is documented. However, little known about female reproduction. This study aims identify circRNAs potentially involved reproductive women’s health. Candidate expressed ovary sponging miRNAs, already be the ovary, were selected by computational approach. Using real time PCR, we verified identified circPUM1 most interesting candidate circRNA for further analyses. We assessed its linear counterpart all follicle compartments and, using experimental approach, direct indirect targets, miRNAs mRNAs, respectively, cumulus cells. found that mRNA host co-expressed proposed potential regulator PTEN, finding strong positive correlation between PTEN mRNA. These results suggest possible cells point out important role inside pathways related growth oocyte maturation.

Language: Английский

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The Role of the Dysregulation of circRNAs Expression in Glioblastoma Multiforme

Journal of Molecular Neuroscience, Journal Year: 2025, Volume and Issue: 75(1)

Published: Jan. 22, 2025

Language: Английский

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The Role of RAS in CNS Tumors: A Key Player or an Overlooked Oncogene?

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4104 - 4104

Published: April 25, 2025

This review examines the prevalence, molecular mechanisms, and clinical implications of RAS mutations in Central Nervous System (CNS) tumors, with a particular focus on glioblastoma. We summarize current understanding RAS-driven oncogenic pathways, their contribution to tumor progression, potential therapeutic strategies targeting its downstream effectors. Although direct are rare primary CNS alterations signaling, such as NF-1 loss aberrant receptor tyrosine kinase activation, contribute malignant progression. Furthermore, emerging evidence links brain metastases, highlighting significance oncology. also discuss recent trials investigating RAS-targeted therapies, including covalent inhibitors, MEK novel combination approaches. Given increasing recognition pathway malignancies, further research is needed elucidate role biology explore targeted interventions.

Language: Английский

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Basic mechanism of mobilizing cell movement during invasion of glioblastoma and target selection of targeted therapy

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Glioblastoma (GBM), also known as glioblastoma multiforme, is a rapidly growing and highly invasive malignant tumor. Due to the inability clearly distinguish between normal tissue, surgery cannot achieve safe resection, often leading poor patient prognosis inevitable tumor recurrence. According previous studies, GBM invasion related intercellular adhesion, matrix degradation, extracellular its adhesion molecules, well molecular of protein hydrolases in microenvironment cells stromal cells. The aim enhance our understanding mechanisms underlying advance research on targeted therapies for inhibiting invasion. This article describes that may affect cell invasion, changes cytoskeleton during motility, regulatory intracellular signaling pathways In addition, we explored possibility therapy against molecules GBM.

Language: Английский

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Profiling the autoantibody repertoire reveals autoantibodies associated with mild cognitive impairment and dementia

Frontiers in Neurology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 30, 2023

Dementia is a debilitating neurological disease affecting millions of people worldwide. The exact mechanisms underlying the initiation and progression remain to be fully defined. There an increasing body evidence for role immune dysregulation in pathogenesis dementia, where blood-borne autoimmune antibodies have been studied as potential markers associated with pathological dementia.This study included plasma from 50 cognitively normal individuals, 55 subjects MCI (mild cognitive impairment), 22 dementia. Autoantibody profiling more than 1,600 antigens was performed using high throughput microarray platform identify differentially expressed autoantibodies dementia.The differential expression analysis identified 33 significantly altered patients dementia compared subjects, 38 MCI. And 20 proteins had autoantibody responses individuals. Five were commonly dysregulated both MCI, including anti-CAMK2A, CKS1B, ETS2, MAP4, NUDT2. Plasma levels anti-ODF3, E6, S100P, ARHGDIG correlated negatively performance scores (MoCA) (r2 -0.56 -0.42, value p < 0.001). Additionally, several targeted by enriched neurotrophin signaling pathway, axon guidance, cholinergic synapse, long-term potentiation, apoptosis, glycolysis gluconeogenesis.We shown multiple corresponding these are involved neurodegenerative pathways, suggesting impact autoimmunity on etiology possible benefit future therapeutic approaches. Further investigations warranted validate our findings.

Language: Английский

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Diagnostic and therapeutic potential of circular RNA in brain tumors

Neuro-Oncology Advances, Journal Year: 2023, Volume and Issue: 5(1)

Published: Jan. 1, 2023

Abstract Circular RNAs (circRNAs) are a class of RNA with stable cyclic structure. They expressed in various tissues and cells conserved, specific characteristics. CircRNAs have been found to play critical roles wide range cellular processes by regulating gene expression at the epigenetic, transcriptional, posttranscriptional levels. There is an accumulation evidence on newly discovered circRNAs, their molecular interactions, development progression human brain tumors, including cell proliferation, apoptosis, invasion, chemoresistance. Here we summarize current state knowledge circRNAs that implicated tumor pathogenesis, particularly gliomas medulloblastomas. In providing comprehensive overview circRNA studies, highlight how different oncogenic or tumor-suppressive making them attractive therapeutic targets biomarkers for personalized therapy precision diagnostics. This review article discusses circRNAs’ functional prospect using as diagnostic patients tumors.

Language: Английский

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MicroRNAs in seminal plasma are able to discern infertile men at increased risk of developing testicular cancer

Molecular Oncology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Male infertility is a risk factor for the development of testicular germ cell tumors. In this study, we investigated microRNA profiles in seminal plasma to identify potential noninvasive biomarkers able discriminate men at highest developing cancer among infertile population. We compared individuals affected by tumors and healthy with normal or impaired spermiograms using high‐throughput technology confirmed results single‐assay digital PCR. found that miR‐221‐3p miR‐222‐3p were downregulated miR‐126‐3p was upregulated patients both fertile men. ROC curve analysis miR‐126 upregulation male addition, in‐depth bioinformatics based on weighted gene co‐expression networks showed identified miRNAs regulate cellular pathways involved cancer.

Language: Английский

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