Role of IL-27 in COVID-19: A Thin Line between Protection and Disease Promotion DOI Open Access
Zoia R. Korobova, N. A. Аrsentieva, Angela Santoni

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7953 - 7953

Published: July 21, 2024

Cytokine storm is usually described as one of the main reasons behind COVID-associated mortality. Cytokines are essential protein molecules engaged in immune responses; they play a critical role protection against infections. However, also contribute to inflammatory reactions and tissue damage, becoming double-edged sword context COVID-19. Recent studies have suggested various cytokines chemokines that crucial response SARS-CoV-2 infection. One such cytokine interleukin 27 (IL-27), which has been found be elevated blood plasma patients with Within this study, we will explore IL-27 responses analyze both existing literature our own prior research findings on It affects wide variety cells. Regardless pathological process it involved in, for upholding necessary balance between damage cytotoxicity infectious agents and/or tumors. In COVID-19, multiple processes, including antiviral via CD8+ cells, IgG subclass switching, even activation Tregs.

Language: Английский

Macrophage-Derived Chemokine MDC/CCL22: An Ambiguous Finding in COVID-19 DOI Open Access
Zoia R. Korobova, N. A. Аrsentieva, Арег А. Тотолян

et al.

Published: May 6, 2023

Macrophage-derived chemokine belongs to the CC subfamily. It is produced by dendritic cells (DCs) and macrophages with or without external stimulation. We have previously shown a statistically significant depletion of MDC/CCL22 concentrations in number studies concerning COVID-19. These shifts demonstrated stability unrelated SARS-CoV-2 genetic variant remained noticeable even convalescent patients. In this work, we analyze dynamics various diseases, including those that manifest inflammation lung tissue. addition, provide our hypothesis on such decrease If its secretion producer unperturbed, then it possible for viral products bind block functional activity. There is, however, another explanation directly linked DC subpopulations inhibition their function. also discuss MDC/CCL22's role immunology novel coronavirus infection, based both own data other studies.

Language: Английский

Citations

1
Effects of Different Types of Recombinant SARS-CoV-2 Spike Protein on Circulating Monocytes’ Structure DOI Open Access
Marco Vettori,

Francesco Dima,

Brandon Michael Henry

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(11), P. 9373 - 9373

Published: May 27, 2023

This study investigated the biological effects on circulating monocytes after challenge with SARS-CoV-2 recombinant spike protein. Whole blood collected from seven ostensibly healthy healthcare workers was incubated for 15 min 2 and 20 ng/mL final concentration of protein Ancestral, Alpha, Delta, Omicron variants. Samples were analyzed Sysmex XN DI-60 analyzers. Cellular complexity (i.e., presence granules, vacuoles other cytoplasmic inclusions) increased in all samples challenged Delta variants, but not those containing Omicron. The cellular content nucleic acids constantly decreased most samples, achieving statistical significance Alpha proteins. heterogeneity monocyte volumes significantly morphological abnormalities included dysmorphia, granulation, intense vacuolization, platelet phagocytosis, development aberrant nuclei, extrusions. triggers important abnormalities, more evident cells clinically severe

Language: Английский

Citations

1
MDC/CCL22 depletion in COVID-19 and post-COVID DOI Creative Commons
Zoia R. Korobova, Арег А. Тотолян

Medical Immunology (Russia), Journal Year: 2023, Volume and Issue: 25(4), P. 773 - 778

Published: June 1, 2023

In this article, we explore the role of macrophage-derived chemokine (MDC/CCL22) in COVID-19 immunity. The study included plasma samples 289 patients with PCR-verified from specialized hospitals. blood were collected at admission, approximately 7 days after start infection. Genetic testing virus was performed nasopharyngeal swabs to determine viral strain for each patient. We also 69 convalescent who had recovered more than a month prior study. Additionally, 51 healthy donors as controls. concentrations MDC/CCL22 and other cytokines chemokines measured multiplex analysis using Luminex MagPix Technology. results showed that significantly lower MDC levels their plasma, regardless SARS-CoV-2 strain, compared donors. This finding suggests depletion may play Furthermore, still decreased infection, indicating persist even recovery. propose two mechanisms can explain reasons leading depletion. first is binding inactivation peptides, making it not only undetectable commercial kits, but less functionally active. Another mechanism dysfunction its effector cells (e.g., DCs macrophages). Lymphopenia following potentially be explained by absence MDC/CCL22. lead shift towards hyperactivation inflammatory response, explaining severity COVID-19. research sheds light on importance immunity highlights need further investigation into disease. Understanding behind could provide new insights pathogenesis inform development potential treatments.

Language: Английский

Citations

1
In-vitro studies of relative intracellular alterations caused by three variants of Severe Acute Respiratory Syndrome Corona Virus-2 (SARS-CoV-2) DOI
Poorna Khaneja, Annette Angel, V. K. Joshi

et al.

Journal of Cellular Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 6

Published: May 8, 2024

BACKGROUND: The comparative understanding between cellular basis of transmission and clinical severities caused by Wuhan, Delta Omicron strains SARS-CoV-2 is important. OBJECTIVE: To conduct In-Vitro studies on infection different variants performing cytotoxic assays studying the live microscopic observations. METHODS: swab samples positive for were inoculated in Vero cell lines their rate infectivity nature cytopathy was observed. RESULTS: study showed maximum inter connectivity among cells infected variant, followed Wuhan least connections variant. strain causing highest mortality as per reported epidemiological trends, damage supporting fastest to spread most patients. CONCLUSIONS: paper reports that numerous cause severities. sensitizes issue 52 subunit viral Spike Protein could act fusion enzyme connections. Study suggests S2 be drug target therapeutic intervention.

Language: Английский

Citations

0
Role of IL-27 in COVID-19: A Thin Line between Protection and Disease Promotion DOI Open Access
Zoia R. Korobova, N. A. Аrsentieva, Angela Santoni

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7953 - 7953

Published: July 21, 2024

Cytokine storm is usually described as one of the main reasons behind COVID-associated mortality. Cytokines are essential protein molecules engaged in immune responses; they play a critical role protection against infections. However, also contribute to inflammatory reactions and tissue damage, becoming double-edged sword context COVID-19. Recent studies have suggested various cytokines chemokines that crucial response SARS-CoV-2 infection. One such cytokine interleukin 27 (IL-27), which has been found be elevated blood plasma patients with Within this study, we will explore IL-27 responses analyze both existing literature our own prior research findings on It affects wide variety cells. Regardless pathological process it involved in, for upholding necessary balance between damage cytotoxicity infectious agents and/or tumors. In COVID-19, multiple processes, including antiviral via CD8+ cells, IgG subclass switching, even activation Tregs.

Language: Английский

Citations

0