The Biochemical Effects of Carotenoids in Orange Carrots on the Colonic Proteome in a Mouse Model of Diet-induced Obesity DOI Open Access

Emilio Balbuena,

Fadia Milhem,

Zeren Kiremitci

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Carotenoids are naturally occurring pigments in plants and responsible for the orange, yellow, red color of fruits vegetables. Carrots one primary dietary sources carotenoids. The biological activities carotenoids higher organisms well documented most tissues but not large intestine. gastrointestinal barrier acts as a line defense against systemic invasion pathogenic bacteria, especially at colonic level. Proteins involved tight junction assembly between epithelial cells mucus secretion from goblet essential maintaining intestinal homeostasis. A high-fat diet can cause gut impairment by inducing permeability, leading to low-grade chronic inflammation via metabolic endotoxemia. Our hypothesis this study is that intake carotenoid-rich foods alleviate obesity-associated strengthen function. Male C57BL/6J mice were randomized four experimental diets 20 weeks (n = animals/group): Low-fat (LFD, 10% calories fat), (HFD, 45% HFD with white carrot powder (HFD + WC), or orange OC). Colon harvested analyze biochemical effects carrots. distal sections subjected isobaric labeling-based quantitative proteomics which tryptic peptides labeled tandem mass tags, followed fractionation LC-MS/MS analysis an Orbitrap Eclipse Tribrid instrument. High-performance liquid chromatography results revealed HFD+WC pellets carotenoid-deficient, HFD+OC contained high concentrations provitamin carotenoids, specifically α-carotene β-carotene. As result proteomics, total 4410 differentially expressed proteins identified. Intestinal barrier-associated highly upregulated group, particularly mucin-2 (MUC-2). Upon closer investigation into mucosal activity, other related MUC-2 functionality management intervention. Collectively, our findings suggest prevent diet-induced disruption promoting synthesis mammalian organisms. Data available ProteomeXchange identifier PXD054150.

Language: Английский

Chemistry, bioavailability, bioactivity, nutritional aspects and human health benefits of polyphenols: A comprehensive review DOI
Mohamed T. El‐Saadony, Tao Yang, Ahmed M. Saad

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134223 - 134223

Published: July 29, 2024

Language: Английский

Citations

28
Short term supplementation with cranberry extract modulates gut microbiota in human and displays a bifidogenic effect DOI Creative Commons
Jacob Lessard‐Lord, Charlène Roussel, Joseph Lupien‐Meilleur

et al.

npj Biofilms and Microbiomes, Journal Year: 2024, Volume and Issue: 10(1)

Published: March 6, 2024

Abstract Cranberry is associated with multiple health benefits, which are mostly attributed to its high content of (poly)phenols, particularly flavan-3-ols. However, clinical trials attempting demonstrate these positive effects have yielded heterogeneous results, partly due the inter-individual variability gut microbiota interaction molecules. In fact, several studies demonstrated ability molecules modulate in animal and vitro models, but there a scarcity information human subjects. addition, it has been recently reported that cranberry also contains concentrations oligosaccharides, could contribute bioactivity. Hence, aim this study was fully characterize (poly)phenolic oligosaccharidic contents commercially available extract evaluate capacity positively 28 After only four days, (poly)phenols oligosaccharides-rich extract, induced strong bifidogenic effect, along an increase abundance butyrate-producing bacteria, such as Clostridium Anaerobutyricum . Plasmatic fecal short-chain fatty acids profiles were altered by decrease acetate ratio butyrate ratio. Finally, variability, we stratified participants according alterations observed following supplementation. Interestingly, individuals having characterized presence Prevotella benefited from Faecalibacterium

Language: Английский

Citations

17
Resveratrol as a privileged molecule with antioxidant activity DOI Creative Commons
Teodora Constantinescu, Alin Grig Mihis

Food Chemistry Advances, Journal Year: 2023, Volume and Issue: 3, P. 100539 - 100539

Published: Nov. 21, 2023

Oxidative stress has a significant impact on the occurrence of many chronic diseases. Antioxidants have ability to prevent, postpone or treat effects free radicals. Resveratrol is polyphenol from class stilbenes. The importance this compound determined by its numerous biological properties. strong antioxidant potential special significance, because way it acts in prevention and treatment action direct, inhibiting production reactive species, indirect, influencing activity enzymes. In case analyzing known methods, was observed that RSV had higher capacity than standard used. Also, vitro vivo studies demonstrate neurodegenerative, cardiovascular renal addition, active preeclampsia infertility. An important aspect fact reduce counteract adverse some anticancer agents.

Language: Английский

Citations

30
Akkermansia muciniphila: biology, microbial ecology, host interactions and therapeutic potential DOI
Athanasia Ioannou, Maryse D Berkhout,

Sharon Y. Geerlings

et al.

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 15, 2024

Language: Английский

Citations

16
Dietary Phenolic Compounds—Wellbeing and Perspective Applications DOI Open Access
Dasha Mihaylova, Maria Dimitrova-Dimova, Aneta Popova

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4769 - 4769

Published: April 27, 2024

Contemporary living is continuously leading to poor everyday choices resulting in the manifestation of various diseases. The benefits plant-based nutrition are undeniable and research on topic rising. Modern man now aware possibilities that plant can provide seeking ways benefit from it. Dietary phenolic compounds among easily accessible beneficial substances exhibit antioxidant, anti-inflammatory, antitumor, antibacterial, antiviral, antifungal, antiparasitic, analgesic, anti-diabetic, anti-atherogenic, antiproliferative, as well cardio-and neuroprotective activities. Several industries exploring incorporate biologically active their produce. This review concentrated presenting current information about dietary contribution maintaining good health. Additionally, this content will demonstrate importance prosperity natural for fields, i.e., food industry, cosmetology, biotechnology, others.

Language: Английский

Citations

14
More than a meat- or synthetic nitrogen fertiliser-substitute: a review of legume phytochemicals as drivers of ‘One Health’ via their influence on the functional diversity of soil- and gut-microbes DOI Creative Commons
Rafael D. C. Duarte, Pietro P. M. Iannetta, Ana Gomes

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 21, 2024

Legumes are essential to healthy agroecosystems, with a rich phytochemical content that impacts overall human and animal well-being environmental sustainability. While these phytochemicals can have both positive negative effects, legumes traditionally been bred produce genotypes lower levels of certain plant phytochemicals, specifically those commonly termed as ‘antifeedants’ including phenolic compounds, saponins, alkaloids, tannins, raffinose family oligosaccharides (RFOs). However, when incorporated into balanced diet, such legume offer health benefits for humans animals. They positively influence the gut microbiome by promoting growth beneficial bacteria, contributing health, demonstrating anti-inflammatory antioxidant properties. Beyond their nutritional value, also play vital role in soil health. The containing residues from shoots roots usually remain in-field affect nutrient status diversity, so enhancing functions benefiting performance yield following crops. This review explores ‘one health’ perspective, examining on soil- gut-microbial ecology, bridging gap between nutrition agroecological science.

Language: Английский

Citations

9
Dietary Polyphenols, Food Processing and Gut Microbiome: Recent Findings on Bioavailability, Bioactivity, and Gut Microbiome Interplay DOI Creative Commons

Monika Sejbuk,

Iwona Mirończuk-Chodakowska, Sercan Karav

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(10), P. 1220 - 1220

Published: Oct. 10, 2024

Polyphenols are organic chemical compounds naturally present in plants, renowned for their anti-inflammatory, antioxidant, immunomodulatory, anticancer, and cardiovascular protective properties. Their bioactivity bioavailability can vary widely depending on the methods of food processing interactions with gut microbiome. These factors induce changes polyphenols, affecting ability to achieve intended health benefits. Thus, it is essential develop apply that optimize polyphenol content while maintaining bioavailability. This review aims explore how various techniques affect quantity, bioactivity, as well microbiome, which may ultimately determine effects.

Language: Английский

Citations

9
The potential of lactic acid bacteria in fermented herbs-derived food products DOI

Haisu Shi,

Yulian Zhao,

Weiming Wang

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 61, P. 104714 - 104714

Published: July 9, 2024

Language: Английский

Citations

7
Assessing the Gut Microbiota's Ability to Metabolize Oligomeric and Polymeric Flavan‐3‐ols from Aronia and Cranberry DOI Creative Commons
Jacob Lessard‐Lord, Charlène Roussel,

Valérie Guay

et al.

Molecular Nutrition & Food Research, Journal Year: 2024, Volume and Issue: 68(5)

Published: Feb. 13, 2024

Abstract Clinical trials investigating the health effects of flavan‐3‐ols yield heterogeneous results due to interindividual variability in gut microbiota metabolism. In fact, different groups population have similar metabolic profiles following (−)‐epicatechin and (+)‐catechin microbial metabolism can be regrouped into so‐called metabotypes. this study, capacity 34 donors metabolize polymeric B‐type from aronia oligomeric A‐type cranberry is investigated by vitro fecal batch fermentations. Less than 1% both sources are converted metabolites, such as phenyl‐γ‐valerolactones (PVLs). To further confirm result, metabolites quantified urine samples collected participants, before after a 4‐day supplementation extract providing 82.3 mg per day. No significant difference observed urinary excretion metabolites. Hence, it demonstrates vivo approaches that poorly degraded microbiota. The beneficial impacts these molecules likely stem their affect interactions with epithelium, rather breakdown smaller

Language: Английский

Citations

6
Epigallocatechin-3-gallate therapeutic potential in human diseases: molecular mechanisms and clinical studies DOI Creative Commons
Manzar Alam,

Mehak Gulzar,

Salman Akhtar

et al.

Molecular Biomedicine, Journal Year: 2024, Volume and Issue: 5(1)

Published: Dec. 27, 2024

Abstract Green tea has garnered increasing attention across age groups due to its numerous health benefits, largely attributed Epigallocatechin 3-gallate (EGCG), key polyphenol. EGCG exhibits a wide spectrum of biological activities, including antioxidant, anti-inflammatory, antibacterial, anticancer, and neuroprotective properties, as well benefits for cardiovascular oral health. This review provides comprehensive overview recent findings on the therapeutic potential in various human diseases. Neuroprotective effects include safeguarding neurons from damage enhancing cognitive function, primarily through antioxidant capacity reduce reactive oxygen species (ROS) generated during physiological stress. Additionally, modulates signaling pathways such JAK/STAT, Delta-Notch, TNF, all which play critical roles neuronal survival, growth, function. Furthermore, is involved regulating apoptosis cell cycle progression, making it promising candidate treatment metabolic diseases, cancer diabetes. Despite potential, further clinical trials are essential validate efficacy safety optimize delivery target tissues. While many reviews have addressed anticancer properties EGCG, this focuses molecular mechanisms by used specific particularly cancer, neurodegenerative It serves valuable resource researchers, clinicians, healthcare professionals, revealing managing disorders, diseases highlighting broader values.

Language: Английский

Citations

6