Mechanistic Role of TRIM26 in Viral Infection and Host Defense DOI Open Access
Mona Sharma, Ke Liu, Jianchao Wei

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(11), P. 1476 - 1476

Published: Nov. 15, 2024

(TRIM26) is an E3 ubiquitin ligase and a member of the TRIM family. Similar to other proteins, TRIM26 consists three domains, collectively termed RBCC: Really Interesting New Gene (RING) domain, one B-Box C terminal domain consisting PRY/SPRY domain. The exhibits relatively higher conservation compared with RING suggesting potentially similar roles across proteins from various species. either directly interacts viral or modulates immune responses engage infection, serving as protective detrimental host factor depending on circumvent infection. present review focuses understanding mechanisms during infection its potential future applications.

Language: Английский

Identification of TRIM21 and TRIM14 as Antiviral Factors Against Langat and Zika Viruses DOI Creative Commons
Pham-Tue-Hung Tran, Mohammad Hazzaz Bin Kabir, Naveed Asghar

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(5), P. 644 - 644

Published: April 29, 2025

Flaviviruses are usually transmitted to humans via mosquito or tick bites, whose infections may lead severe diseases and fatality. During intracellular infection, they remodel the endoplasmic reticulum (ER) membrane generate compartments scaffolding replication complex (RC) where of viral genome takes place. In this study, we purified ER fraction virus infected cells identify proteins that were enriched during flavivirus infection. We found tripartite motif-containing (TRIMs) including TRIM38, TRIM21, TRIM14 significantly infection with mosquito-borne (West Nile strain Kunjin Zika (ZIKV)) tick-borne (Langat (LGTV)) flaviviruses. Further characterizations showed TRIM21 act as restriction factors against ZIKV LGTV, while TRIM38 hinders These TRIMs worked interferon-stimulated genes mediate IFN-I response LGTV infections. Restriction by coincides their colocalization NS3. TRIM14-mediated its NS3 NS5 proteins. However, did not colocalize protein suggesting antiviral activity is dependent on direct targeting enzyme. Finally, demonstrated overexpression restricted replication.

Language: Английский

Citations

0
Targeting Stat3 with conditional knockout or PROTAC technology alleviates renal injury by Limiting pyroptosis DOI

Ming‐lu Ji,

Jianan Wang,

Mingfei Wu

et al.

EBioMedicine, Journal Year: 2025, Volume and Issue: 116, P. 105739 - 105739

Published: May 9, 2025

Language: Английский

Citations

0
Ro52/TRIM21 – From host defense to autoimmunity DOI

Emilia Holwek,

Aleksandra Opinc, Joanna Sarnik

et al.

Cellular Immunology, Journal Year: 2023, Volume and Issue: 393-394, P. 104776 - 104776

Published: Oct. 15, 2023

Language: Английский

Citations

5
TRIM21 and Fc-engineered antibodies: decoding its complex antibody binding mode with implications for viral neutralization DOI Creative Commons

Johannes Reusch,

Linda E. Franken,

Jakob Then

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: June 12, 2024

TRIM21 is a pivotal effector in the immune system, orchestrating antibody-mediated responses and modulating signaling. In this comprehensive study, we focus on interaction of with Fc engineered antibodies subsequent implications for viral neutralization. Through series analytical techniques, including biosensor assays, mass photometry, electron microscopy, along structure predictions, unravel intricate mechanisms governing interplay between antibodies. Our investigations reveal that capacity to recognize, bind, facilitate proteasomal degradation antibody-coated viruses critically dependent affinity avidity its interactions antibody regions. We suggest novel binding mechanism, where one site results detachment PRYSPRY from coiled-coil domain, enhancing mobility due flexible linker, thereby facilitating engagement second site, resulting bivalent engagement. These findings shed light dual role antiviral immunity, both recognizing directing intracellular degradation, demonstrate potential therapeutic exploitation. The study advances our understanding opens new avenues development strategies innovation tailored functions designed leverage TRIM21s unique mode.

Language: Английский

Citations

1
The Interactions between Cells and Viruses DOI Open Access
Masahiro Fujimuro

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 6886 - 6886

Published: June 23, 2024

Many infectious diseases are caused by life-threatening DNA and RNA viruses have been reported worldwide, including those emerging re-emerging [...]

Language: Английский

Citations

1
FAT10 inhibits TRIM21 to down-regulate antiviral type-I interferon secretion DOI Creative Commons
Kritika Saxena, Katharina Inholz, Michael Basler

et al.

Life Science Alliance, Journal Year: 2024, Volume and Issue: 7(9), P. e202402786 - e202402786

Published: July 8, 2024

The ubiquitin-like modifier FAT10 is upregulated under pro-inflammatory conditions, targets its substrates for proteasomal degradation and functions as a negative regulator of the type-I IFN response. Influenza A virus infection upregulates production expression E3 ligase TRIM21, which regulates in positive feedback manner. In this study, we show that becomes covalently conjugated to TRIM21 degradation. We further coiled-coil PRYSPRY domains C-terminal diglycine motif are important TRIM21-FAT10 interaction. Moreover, upon influenza presence total ubiquitination reduced our data reveal FAT10-mediated diminishes IFNβ production. Overall, study provides strong evidence down-regulates antiviral by modulating additional molecules RIG-I signaling pathway besides already published OTUB1. addition, elucidate novel mechanism stability.

Language: Английский

Citations

1
TRIM21 of Micropterus salmoides exerts antiviral roles against largemouth bass ulcer syndrome virus DOI

Zichao Peng,

Caiyun Zhang,

Baojie Yin

et al.

Fish & Shellfish Immunology, Journal Year: 2023, Volume and Issue: 142, P. 109176 - 109176

Published: Oct. 18, 2023

Language: Английский

Citations

3
Human super antibody to viral RNA-dependent RNA polymerase produced by a modified Sortase self-cleave-bacteria surface display system DOI Creative Commons
Kittirat Glab-ampai, Kodchakorn Mahasongkram, Monrat Chulanetra

et al.

Microbial Cell Factories, Journal Year: 2023, Volume and Issue: 22(1)

Published: Dec. 18, 2023

RNA-dependent RNA polymerase (RdRp) is a good target of anti-RNA virus agents; not only it pivotal for the replication cycle and highly conserved among viruses across different families, but also lacks human homolog. Recently, single-chain antibody (HuscFv) that bound to thumb domain hepatitis C (HCV) (functionalized NS5B protein) was produced engineered into cell-penetrating (super antibody) in form peptide (penetratin, PEN)-linked HuscFv (PEN-HuscFv34). The super purified from inclusion body (IB) pen-huscfv34-vector-transformed Escherichia coli. inhibited alpha- beta- coronaviruses, flaviviruses, picornaviruses were tested (broadly effective); thus, has high potential developing further towards pan-anti-RNA agent. However, production, purification, refolding molecules bacterial IB are laborious hurdles large-scale production. Therefore, this study, Sortase-self-cleave method bacteria surface display system combined modified production.BL21 (DE3) ΔA E. coli, strain lacking predominant outer membrane protein (OmpA) ion OmpT proteases, displayed membrane-anchored fusion protein, i.e., chimeric lipoprotein (Lpp')-OmpA', SUMO, Sortase protease, cleavage site (LPET↓G) PEN-HuscFv34-6× His generated. soluble with glycine at N-terminus could be released coli surface, simply by incubating cells Sortase-cleavage buffer. After centrifugation, G-PEN-HuscFv34-6× supernatant. retained original ability (being broadly effective activity IB-derived-PEN-HuscFv34.The functionalized RdRp successfully using self-cleave systems modification. suitable downstream processing production antibody. It applicable other recombinant proteins free-folding form.

Language: Английский

Citations

1
Mechanistic Role of TRIM26 in Viral Infection and Host Defense DOI Open Access
Mona Sharma, Ke Liu, Jianchao Wei

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(11), P. 1476 - 1476

Published: Nov. 15, 2024

(TRIM26) is an E3 ubiquitin ligase and a member of the TRIM family. Similar to other proteins, TRIM26 consists three domains, collectively termed RBCC: Really Interesting New Gene (RING) domain, one B-Box C terminal domain consisting PRY/SPRY domain. The exhibits relatively higher conservation compared with RING suggesting potentially similar roles across proteins from various species. either directly interacts viral or modulates immune responses engage infection, serving as protective detrimental host factor depending on circumvent infection. present review focuses understanding mechanisms during infection its potential future applications.

Language: Английский

Citations

0