Microalgae-Derived Microparticles Improve Immunomodulation via Combined Glycolysis and MAPK Activation

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Natural polysaccharides possess potent immunostimulatory properties, but their poor solubility impedes efficiency of cellular delivery. This study focuses on extraction microparticles (MPs) fromEuglena gracilis, a microalgae species characterized by abundant intracellular β-1,3-glucan and flexible cell membrane. We introduce anE. gracilis-derived MP (MPEG) system as natural carrier for solubilized β-glucan. The MPEG enhances β-glucan's loading through sequential sonication extrusion. In vitro studies reveal that utilizes multiple endocytosis pathways, including phagocytosis, clathrin-mediated, lipid raft-mediated routes, effective β-glucan delivery into cells. Upon internalization, components trigger dual activation the MAPK signaling pathway glycolysis in macrophages, leading to enhanced production pro-inflammatory cytokines lactic acid, ultimately strengthening innate immune responses. offers promising approach harnessing properties while overcoming limitations, opening new avenues targeted immunomodulation therapies.

Language: Английский

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Microalgae biotechnological potentials

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 757 - 804

Published: Jan. 1, 2025

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Closed Fixed-Bed Bacteria-Algae Biofilm Reactor: A Promising Solution for phenol containing wastewater Treatment and Resource Transformation

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 138176 - 138176

Published: April 1, 2025

Language: Английский

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Microalgae as an emerging alternative raw material of docosahexaenoic acid and eicosapentaenoic acid – a review

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 20

Published: April 6, 2025

Long-chain omega-3 polyunsaturated fatty acids (n-3 PUFAs) have been widely applied due to their nutraceutical and healthcare benefits. With the rising rates of chronic diseases, there is a growing consumer interest demand for sustainable dietary sources n-3 PUFAs. Currently, microalgae emerged as source PUFAs which are rich in docosahexaenoic acid (DHA) eicosapentaenoic (EPA), regarded promising alternatives conventional (seafood) that cannot meet demands natural food supplements. This review provides comprehensive overview recent advancements strategies such genetic engineering, mutagenesis, improving photosynthetic efficiency, nutritional or environmental factors, cultivation approaches improve DHA EPA production efficiency cells. Additionally, it explains application EPA-rich animal feed, human nutrition- snacks, supplements avoid malnutrition non-communicable diseases.

Language: Английский

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In Vitro Culture, Genetic Transformation and the Production of Biopharmaceuticals in Microalgae

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3890 - 3890

Published: April 20, 2025

Microalgae represent a promising platform for the synthesis of recombinant proteins, particularly in context biopharmaceutical applications. Their unique combination eukaryotic cellular machinery and prokaryotic-like simplicity offers several advantages, including ability to perform complex post-translational modifications, rapid growth rates, cost-effective culture conditions. Advances genome sequencing, genetic engineering tools, omics technologies have significantly enhanced feasibility efficiency using microalgae therapeutic protein production. These advancements, coupled with development well-established transformation methods optimized vectors, enabled successful expression various biopharmaceuticals, ranging from vaccines enzymes. Here, main stages current status production exogenic proteins dedicated human therapy are presented.

Language: Английский

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Identification and Functional Analysis of Two Novel Genes—Geranylgeranyl Pyrophosphate Synthase Gene (AlGGPPS) and Isopentenyl Pyrophosphate Isomerase Gene (AlIDI)—from Aurantiochytrium limacinum Significantly Enhance De Novo β-Carotene Biosynthesis in Escherichia coli

Marine Drugs, Journal Year: 2023, Volume and Issue: 21(4), P. 249 - 249

Published: April 17, 2023

Precursor regulation has been an effective strategy to improve carotenoid production and the availability of novel precursor synthases facilitates engineering improvements. In this work, putative geranylgeranyl pyrophosphate synthase encoding gene (AlGGPPS) isopentenyl isomerase (AlIDI) from Aurantiochytrium limacinum MYA-1381 were isolated. We applied excavated AlGGPPS AlIDI de novo β-carotene biosynthetic pathway in Escherichia coli for functional identification application. Results showed that two genes both functioned synthesis β-carotene. Furthermore, performed better than original or endogenous one, with 39.7% 80.9% increases production, respectively. Due coordinated expression 2 genes, content modified carotenoid-producing E. accumulated a 2.99-fold yield initial EBIY strain 12 h, reaching 10.99 mg/L flask culture. This study helped broaden current understanding provided elements

Language: Английский

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Development of Chlamydomonas sp. biorefinery for sustainable methyl ester and malic acid production

Fuel, Journal Year: 2024, Volume and Issue: 371, P. 132017 - 132017

Published: May 28, 2024

Language: Английский

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Photosynthetic response mechanism to polybrominated diphenyl ether exposure in Chlorella pyrenoidosa

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 263, P. 115245 - 115245

Published: July 12, 2023

Polybrominated diphenyl ether (PBDE) contamination is common in aquatic environments and can severely damage organisms. However, there a lack of information on the response self-adaptation mechanisms these Chlorella pyrenoidosa was treated with 2,2′,4,4′-tetrabromodiphenyl (BDE47), causing significant growth inhibition, pigment reduction, oxidative stress, chloroplast atrophy. Photosynthetic contributed to as indicated by Fv/Fm, Chl fluorescence induction, photosynthetic oxygen evolution activity, photosystem subunit stoichiometry. Here, induction quinone electron acceptor (QA−) reoxidation kinetics showed that PSII donor sides were insensitive BDE47. Quantitative analyses D1 PsaD proteins illustrated PSI complexes main primary targets photosynthesis inhibition Significant modulation complex might have been caused potential binding BDE47 protein, molecular docking performed investigate this. Increased activation antioxidant defense systems repair function exposure time positive resistance After 5-day exposure, 23 % metabolized. Our findings suggest C. has bioremediator for wastewater-borne PBDEs improve our understanding ecological risks microalgae.

Language: Английский

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Triazine herbicide reduced the toxicity of the harmful dinoflagellate Karenia mikimotoi by impairing its photosynthetic systems

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 269, P. 115740 - 115740

Published: Dec. 1, 2023

Triazine herbicides are common contaminants in coastal waters, and they recognized as inhibitors of photosystem II, causing significant hinderance to the growth reproduction phytoplankton. However, influence these on microalgal toxin production remains unclear. This study aimed examine this relationship by conducting a comprehensive physiological 4D label-free quantitative proteomic analysis harmful dinoflagellate Karenia mikimotoi presence triazine herbicide dipropetryn. The findings demonstrated decrease photosynthetic activity pigment content, well reduced levels unsaturated fatty acids, reactive oxygen species (ROS), hemolytic toxins K. when exposed revealed down-regulation proteins associated with photosynthesis, ROS response, energy metabolism, such acid biosynthesis, chlorophyll nitrogen metabolism. In contrast, an up-regulation related energy-producing processes, β-oxidation, glycolysis, tricarboxylic cycle, was observed. that dipropetryn disrupts systems mikimotoi, resulting notable algal production. These provide valuable insights into underlying mechanisms toxigenic microalgae explore potential effect pollution blooms environments.

Language: Английский

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Recombinant protein synthesis and isolation of human interferon alpha-2 in cyanobacteria

Bioresource Technology, Journal Year: 2024, Volume and Issue: 400, P. 130664 - 130664

Published: April 5, 2024

Synechocystis sp. PCC 6803 (Synechocystis) is a unicellular photosynthetic microorganism that has been used as model for photo-biochemical research. It comprises potential cell factory the generation of valuable bioactive compounds, therapeutic proteins, and possibly biofuels. Fusion constructs recombinant proteins with CpcA α-subunit or CpcB β-subunit phycocyanin in have enabled true over-expression several isoprenoid pathway enzymes biopharmaceutical to levels 10-20 % total cellular protein. The present work employed human interferon α-2 protein, study case downstream processing. advanced state art fusion protein overexpression technology by developing bioresource target separation from construct isolation substantially enriched pure form. brings cyanobacterial concept closer meaningful commercial application production useful proteins.

Language: Английский

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