Recent Advances on Key Enzymes of Microbial Origin in the Lycopene Biosynthesis Pathway DOI

Bangmian Du,

Mengjuan Sun,

Wenyang Hui

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(35), P. 12927 - 12942

Published: Aug. 23, 2023

Lycopene is a common carotenoid found mainly in ripe red fruits and vegetables that widely used the food industry due to its characteristic color health benefits. Microbial synthesis of lycopene gradually replacing traditional methods plant extraction chemical as more economical productive manufacturing strategy. The biosynthesis typical multienzyme cascade reaction, it important understand characteristics each key enzyme involved how they are regulated. In this paper, catalytic enzymes pathway related studies first discussed detail. Then, strategies applied synthesis, including fusion proteins, screening, combinatorial engineering, CRISPR/Cas9-based gene editing, DNA assembly, scaffolding technologies purposefully illustrated compared terms both emerging regulatory strategies. Finally, future developments options for similar secondary metabolites also discussed.

Language: Английский

Marine thraustochytrid: exploration from taxonomic challenges to biotechnological applications DOI Creative Commons
Qiuzhen Wang, Yong Zhang,

Ruixue Hui

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: March 22, 2024

Thraustochytrids, as a distinct group of heterotrophic protists, have garnered considerable attention owing to their remarkable adaptability in extreme marine environments, pronounced capacity for metabolic regulation and prolific production high-value metabolites. The taxonomic classification these microorganisms presents substantial challenge due the variability morphological characteristics under different culture conditions. And this undermines efficacy traditional systems on physiological biochemical traits. establishment polyphasic system integrating genomic future will provide new avenues more accurate identification. Thraustochytrids can effectively accumulate bioactive substances such docosahexaenoic acid (DHA), eicosapentaenoic (EPA), squalene carotenoids. Through fermentation optimization genetic modification, scientists significantly enhanced Moreover, application thraustochytrids aquaculture, poultry livestock feed has improved animal growth indicators meanwhile increasing DHA content. Natural thraustochytrids, terpenoid compounds with antioxidant properties, been proposed cosmetics industry. In field pharmacology, shown certain anti-inflammatory anti-cancer activities potential development oral vaccines. Additionally, they degrade various industrial agricultural wastes fatty production, demonstrating environmental bioremediation. Therefore, not only exhibit tremendous biotechnology, but also hold significant value protection commercialization.

Language: Английский

Citations

8
Transcending membrane barriers: advances in membrane engineering to enhance the production capacity of microbial cell factories DOI Creative Commons
Tao Wu, Jingjing Jiang, Hongyang Zhang

et al.

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: May 25, 2024

Microbial cell factories serve as pivotal platforms for the production of high-value natural products, which tend to accumulate on membrane due their hydrophobic properties. However, limited space presents a bottleneck accumulation these products. To enhance intracellular products and alleviate burden caused by product accumulation, researchers have implemented various engineering strategies. These strategies involve modifying components structures microbial achieve efficient target This review summarizes recent advances in application technologies factories, providing case studies involving Escherichia coli yeast. Through strategies, not only improved tolerance cells but also optimized storage space, significantly enhancing efficiency article aims provide scientific evidence references further similar factories.

Language: Английский

Citations

5
Strategies for the efficient biosynthesis of β-carotene through microbial fermentation DOI
Jingnan Wang,

Wenqi Ma,

Weixu Ma

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(5)

Published: April 12, 2024

Language: Английский

Citations

4
Multi-Omics Analysis Provides Insights into a Mosaic-Leaf Phenotype of Astaxanthin-Producing Tobacco DOI Creative Commons
Jialin Wang,

Zaifeng Du,

Xiaoyang Lin

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 965 - 965

Published: March 19, 2025

In metabolically engineered plants, the target products are usually uniformly distributed in whole plant or specific tissues. When engineering tobacco to produce astaxanthin, a ketocarotenoid with strong antioxidant activity and multiple bioactivities, scattered distribution of astaxanthin-producing regions was observed small portion which caused mosaic-like red green spots on leaves (ASTA-mosaic). A physiological assay showed that non-astaxanthin region (Mosaic_G) had relatively higher chlorophyll content better chloroplast structure than (Mosaic_R). Then, metabolomics, proteomics, RNA transcriptomics were employed analyze uneven leaves. The results metabolomics proteomics revealed decrease carotenoid metabolism, biosynthesis, degradation Mosaic_G region. Pheophorbide a, an intermediate degradation, found be significantly reduced region, accompanied by attenuation chlorophyllase pheophytinase, catalyze formation pheophorbide degradation. Reductions photosynthetic antenna proteins photosystem-associated Mosaic_R consistent Small several RNAs could chlorophyll-degradative genes, but they more effective targeting astaxanthin biosynthetic genes. This finding supported fact can remain up senescence work provides insights into mechanism may contribute specialized utilization plants for metabolic engineering.

Language: Английский

Citations

0
Reprogramming Schizochytrium cell factory for High-Yield production of astaxanthin via mutagenesis and metabolic engineering DOI
Yi Hu, Yu Duan, Yuan Zhang

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 163556 - 163556

Published: May 1, 2025

Language: Английский

Citations

0
Recent Advances on Key Enzymes of Microbial Origin in the Lycopene Biosynthesis Pathway DOI

Bangmian Du,

Mengjuan Sun,

Wenyang Hui

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(35), P. 12927 - 12942

Published: Aug. 23, 2023

Lycopene is a common carotenoid found mainly in ripe red fruits and vegetables that widely used the food industry due to its characteristic color health benefits. Microbial synthesis of lycopene gradually replacing traditional methods plant extraction chemical as more economical productive manufacturing strategy. The biosynthesis typical multienzyme cascade reaction, it important understand characteristics each key enzyme involved how they are regulated. In this paper, catalytic enzymes pathway related studies first discussed detail. Then, strategies applied synthesis, including fusion proteins, screening, combinatorial engineering, CRISPR/Cas9-based gene editing, DNA assembly, scaffolding technologies purposefully illustrated compared terms both emerging regulatory strategies. Finally, future developments options for similar secondary metabolites also discussed.

Language: Английский

Citations

4