Transcriptome-wide analysis reveal dynamic expression changes during endoplasmic reticulum stress in tomato DOI

Elif Pulat,

Özgür Çakır

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 13

Published: Nov. 5, 2024

Endoplasmic reticulum (ER) stress is a cellular condition induced by environmental stressors. Transcriptomic approaches reveal the response of plants to ER and provide broadened view. Tomato were treated with tunicamycin, total RNA isolated from tomato leaves was sequenced on an RNA-sequencing platform be analyzed for differential expressions. A 856 differentially expressed genes (DEGs) discovered in tunicamycin-treated leaves. Upregulation marker gene expressions detected after 2h, while prolonged downregulated most protein-coding genes. Pathways such as: stress, jasmonic acid mediated signaling, signal transduction regulation, plant hormone transduction, protein processing enriched. Prolonged mostly resulted reduced transcript levels, possibly related mechanism functioning lower load nascent transcripts ER. Our findings emphasize key role JA-regulated signaling responses due pathway components, along other hormones signaling-related or regulatory being common many stressors plants, transcriptome data obtained here will further studies understanding tolerance generating stress-resistant plants.

Language: Английский

Revitalizing agriculture: next-generation genotyping and -omics technologies enabling molecular prediction of resilient traits in the Solanaceae family DOI Creative Commons
Matteo Martina, Valeria De Rosa, Gabriele Magon

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 5, 2024

This review highlights -omics research in Solanaceae family, with a particular focus on resilient traits. Extensive has enriched our understanding of genomics and genetics, historical varietal development mainly focusing disease resistance cultivar improvement but shifting the emphasis towards unveiling resilience mechanisms genebank-preserved germplasm is nowadays crucial. Collecting such information, might help researchers breeders developing new experimental design, providing an overview state art most advanced approaches for identification genetic elements laying behind resilience. Building this starting point, we aim at useful tool tackling global agricultural goals these crops.

Language: Английский

Citations

5
Exploring the Antifungal Activity of Moroccan Bacterial and Fungal Isolates and a Strobilurin Fungicide in the Control of Cladosporium fulvum, the Causal Agent of Tomato Leaf Mold Disease DOI Creative Commons
Zineb Belabess,

Bilale Gajjout,

Ikram Legrifi

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(16), P. 2213 - 2213

Published: Aug. 9, 2024

The causal agent of tomato leaf mold, Cladosporium fulvum, is prevalent in greenhouses worldwide, especially under high humidity conditions. Despite its economic impact, studies on antifungal agents targeting C. fulvum remain limited. This study evaluates biocontrol (BCAs) as alternatives to chemical controls for managing this disease, alongside the strobilurin fungicide azoxystrobin. From a Moroccan collection potential BCAs, five bacterial isolates (Alcaligenes faecalis ACBC1, Pantoea agglomerans ACBC2, ACBP1, ACBP2, and Bacillus amyloliquefaciens SF14) three fungal (Trichoderma spp. OT1, AT2, BT3) were selected tested. vitro results demonstrated that P. reduced mycelial growth by over 60% at 12 days post-inoculation (dpi), while Trichoderma achieved 100% inhibition just 5 dpi. All produced volatile organic compounds (VOCs) with rates ranging from 38.8% 57.4%. Likewise, cell-free filtrates significantly inhibited pathogen’s growth. Greenhouse tests validated these findings, showing all tested effective reducing disease incidence severity. Azoxystrobin effectively impeded growth, particularly protective treatments. Fourier transform infrared spectroscopy (FTIR) analysis revealed significant biochemical changes treated plants, indicating activity. provides valuable insights into efficacy BCAs azoxystrobin, contributing integrated management strategies mold disease.

Language: Английский

Citations

3
Tomato mitogen-activated protein kinase: mechanisms of adaptation in response to biotic and abiotic stresses DOI Creative Commons

Yumei Shi,

Zhifang Zhang, Zhiping Yan

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 3, 2025

Plants live under various biotic and abiotic stress conditions, to cope with the adversity severity of these they have developed well-established resistance mechanisms. These mechanisms begin perception stimuli, followed by molecular, biochemical, physiological adaptive measures. Tomato ( Solanum lycopersicum ) is a globally significant vegetable crop that experiences several events can adversely impact its quality production. Mitogen-activated protein kinases (MAPKs) in tomato plants crucial functions mediating responses environmental cues, internal signals, defense mechanisms, cellular processes, plant development growth. MAPK cascades respond factors modulating associated gene expression, influencing hormone synthesis, facilitating interactions other stressors. Here, we review evolutionary relationships 16 SlMAPK family members emphasize on recent studies describing regulatory SlMAPKs both conditions. This could enhance our comprehension network conditions provide theoretical support for breeding tomatoes agronomic traits excellent resistance.

Language: Английский

Citations

0
Moss-pathogen interactions: a review of the current status and future opportunities DOI Creative Commons
Huan Zhang, Qilin Yang, Le Yi Wang

et al.

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 11, 2025

In complex and diverse environments, plants face constant challenges from various pathogens, including fungi, bacteria, viruses, which can severely impact their growth, development, survival. Mosses, representing early divergent lineages of land plants, lack traditional vascular systems yet demonstrate remarkable adaptability across habitats. While sharing the fundamental innate immune common to all mosses have evolved distinct chemical physical defense mechanisms. Notably, they exhibit resistance many pathogens that typically affect plants. Their evolutionary significance, relatively simple morphology, well-conserved mechanisms make excellent model organisms for studying plant-pathogen interactions. This article reviews current research on moss-pathogen interactions, examining host-pathogen specificity, characterizing infection phenotypes physiological responses, comparing pathogen susceptibility between angiosperms. Through this analysis, we aim deepen our understanding plant system evolution potentially inform innovative approaches enhancing crop disease resistance.

Language: Английский

Citations

0
Sustainable responses to open field tomato (Solanum lycopersicum L.) stress impacts DOI Creative Commons
Mohammed Mustafa,

Ruth W. Mwangi,

Zita Szalai

et al.

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101825 - 101825

Published: March 1, 2025

Language: Английский

Citations

0
Hazelnut allergome overview and Cor a gRNAs identification DOI Creative Commons
Ciro Gianmaria Amoroso,

Giuseppe Andolfo

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 19, 2025

Corylus species (hazelnuts) are a valuable source of nutrients and widely consumed worldwide. Nevertheless, avellana (Cor a) contains 13 allergens 1, Cor 2, 6, 8, 9, 10, 11, 12, 13, 14, 15, 16, TLP) that have been deposited into the official database (WHO/IUIS) for allergen nomenclature. The recent availability several genomes provided opportunities to explore allergome variability, thus develop hypoallergenic varieties using modern biotech approaches. Certainly, identification CRISPR-Cas9 guide RNA (gRNA) is pivotal step in achieving this goal. User-friendly web tools include limited reference design gRNAs, while bioinformatic software custom analysis require advanced command-line skills. This work explored evolutionary trajectories allergenic homologs C. avellana, americana, heterophylla, mandshurica genome assemblies. 52 orthologs were found analyzed species, tandem duplication 1 was americana. Three new gene models predicted 16 10. Additionally, we identified 56 isoallergens, which ten isoforms. Furthermore, phylogenetic sheds light on dynamics three hazelnut revealing complexity TLP within genus. A list multiple gRNAs designed system singular silencing each genome. study enhances our knowledge path among provides highly accurate on-target guides targeting allergome.

Language: Английский

Citations

0
Seed storage allergens tackled via next-generation research assistant DOI Creative Commons

Adriana Rita Evangelista,

Ciro Gianmaria Amoroso, Chiara Nitride

et al.

Frontiers in Food Science and Technology, Journal Year: 2024, Volume and Issue: 4

Published: March 12, 2024

The expanding consumption of plant proteins in the diet to overcome environmental issues associated with animal is increasing incidence food-induced allergic reactions. One 21st-century research drivers agriculture sciences development and validation concrete approaches for modulating expression allergenic crops before harvesting. food allergies primarily induced by seed storage that clinicians are experiencing recently because more predominant use plant-derived industry. Increased availability high-throughput technologies has generated an ever-growing number omics data, allowing us have better structural knowledge SSPs molecular properties can inform allergenicity assessment. recent systems targeted genome engineering, without double-strand DNA breaks, allow introduction precise modifications directly into commercial species. Artificial intelligence significantly transforming scientific across every stage, assisting scientists, processing large-scale making predictions, automating tasks. During this epochal change, marked encounter between artificial synthetic biology, a next-generation assistant (NGA) coming alive. Here, we propose new conceptual vision facilitate speed up editing cross-reactivity sites obtain hypoallergenic cultivars avoid pleiotropic effects. Finally, discuss potential applications way conceive research. NGA may be undoubtedly capable managing evolution SPP through prediction novel epitopes, as well immunological response mechanisms.

Language: Английский

Citations

2
Genetic engineering in tomato DOI
Sana Tariq, Alvina Gul, Stefano Negri

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 101 - 133

Published: Jan. 1, 2024

Language: Английский

Citations

1
Characterisation of a major QTL for sodium accumulation in tomato grown in high salinity DOI

Alexandre Héreil,

Michel Guillaume,

Renaud Duboscq

et al.

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: 47(12), P. 5089 - 5103

Published: Aug. 15, 2024

Soil salinity is a serious concern for tomato culture, affecting both yield and quality parameters. Although some genes involved in salt tolerance have been identified, their genetic diversity has rarely studied. In the present study, we assessed tolerance-related traits at juvenile adult stages large core collection identified quantitative trait loci (QTLs) by genome-wide association study (GWAS). The results suggested that major QTL leaf sodium accumulation physiological stages. We were able to identify underlying candidate gene, coding well-known transporter, called SlHKT1.2. showed an eQTL expression of this gene roots colocalized with above ground content QTL. A polymorphism putatively responsible its variation was promoter. Finally, extend applicability these results, carried out same analysis on test-cross panel composed crossed distant line. indicated retained functional impact even hybrid context: paves way use breeding programs aimed improving cultivars.

Language: Английский

Citations

1
SEL1L3 as a link molecular between renal cell carcinoma and atherosclerosis based on bioinformatics analysis and experimental verification DOI Creative Commons
Haoyuan Wang,

Xiaopeng Ma,

Sijie Li

et al.

Aging, Journal Year: 2023, Volume and Issue: 15(22), P. 13150 - 13162

Published: Nov. 21, 2023

Renal cancer, the most common type of kidney develops in renal tubular epithelium. Atherosclerosis aorta is primary cause atherosclerosis. However, underlying mechanisms remain unclear.The clear cell carcinoma RNA sequence profile was obtained from The Cancer Genome Atlas (TCGA) database, and atherosclerosis datasets GSE28829 GSE43292 based on GPL570 GPL6244 Gene Expression Omnibus (GEO) database. difference hub genes were identified by Limma protein-protein interaction (PPI) network R software. Functional enrichment, survival, immunoinfiltration analyses performed. role SEL1L3 ErbB/PI3K/mTOR signaling pathway, apoptosis, invasion, cycle, inflammation analyzed using western blotting.764 DEGs TCGA Kidney Clear Cell Carcinoma (KIRC) dataset. A total 344 117 screened GSE14762 GSE53757 datasets, respectively. enrichment analysis results primarily indicated transporter complex, DNA-binding transcription activator activity, morphogenesis embryonic epithelium, stem proliferation, adrenal overactivity so on. Fifteen overlapped among three datasets. PPI revealed that core gene. Survival showed lower expression levels led to a worse prognosis. Immune infiltration significantly correlated with antibody-drug conjugates (aDC), B cells, eosinophils, interstitial dendritic cells (iDC), macrophages, more.SEL1L3 plays an important may be potential link between them.

Language: Английский

Citations

3