ERJ Open Research,
Journal Year:
2024,
Volume and Issue:
11(1), P. 00347 - 2024
Published: Oct. 10, 2024
Sputum
extracellular
DNA
(eDNA)
is
associated
with
disease
severity
in
asthma
and
COPD
therefore
emerging
as
a
potential
therapeutic
target.
The
aim
of
this
study
was
to
investigate
the
effect
10
days
recombinant
human
DNase
(rhDNase)
treatment
eDNA-high
on
sputum
eDNA
levels,
neutrophil-related
inflammation,
lung
function
symptoms.
Adults
(n=80)
or
(n=66)
were
screened
for
presence
high
(>20
µg·mL-1)
those
eligible
(n=18
asthma,
n=17
COPD)
randomised
two-period
crossover
controlled
trial
consisting
daily
nebulised
rhDNase
(2.5
mg/2.5
mL)
placebo
(5
mL
0.9%
saline)
days,
2-week
washout
period.
primary
outcome
eDNA,
secondary
outcomes
included
neutrophil
trap
(NET)-related
biomarkers,
inflammatory
cell
counts,
respiratory
At
screening,
significantly
higher
total
count,
colour
score
inflammation
(HNP1-3,
LL-37
interleukin-1β)
both
compared
low
groups.
In
participants
older
had
poorer
control
eDNA.
Administration
reduced
levels
(median
(Q1-Q3)
Pre:
48.4
(22.1-74.1);
Post:
17.0
(5.0-31.0)
µg·mL-1;
p=0.022)
39.3
(36.7-55.6);
25.4
(11.3-38.6)
p=0.044)
placebo.
Symptoms,
NET
biomarkers
remained
unchanged.
there
reduction
banded
blood
neutrophils
(3.2
(0-7.7)
0.0
(0.0-1.5);
p=0.044).
Targeted
effectively
COPD.
European Journal of Immunology,
Journal Year:
2025,
Volume and Issue:
55(4)
Published: April 1, 2025
Neutrophils
interact
with
and
activate
fibroblasts
through
the
release
of
neutrophil
extracellular
traps
(NETs).
We
investigated
role
NETs-fibroblast
crosstalk
in
cutaneous
wound
healing
type
2
diabetes
(T2D).
Neutrophils/NETs,
serum,
primary
human
skin
(HSFs)
were
obtained
from
individuals
T2D
age/sex-matched
controls.
NET-stimulation
studies
performed
on
neutrophils/HSFs,
without
specific
inhibitors,
while
HSF
capacity
was
assessed
using
a
scratch
assay.
HSFs
display
profibrotic
phenotype,
showing
increased
CCN2/CTGF,
α-smooth
muscle
actin,
collagen
release,
albeit
impaired
capacity,
elevated
I
C-terminal
telopeptide,
degradation
associated
(∼3.5-fold)
matrix
metalloproteinase-9
(MMP-9)
neutrophils/NETs.
IL-8
induced
expression
MMP-9
Moreover,
neutrophils/NETs
exhibited
content,
which
acted
an
autocrine/paracrine
fashion
to
further
augment
its
production
by
neutrophils/HSFs.
The
findings
validated
normoglycemic
during
hyperglycemic
clamp
concomitant
lipid
infusion
corroborated
immunohistochemically
diabetic
plantar
ulcer
biopsies.
This
novel,
vicious
circle
NETs/interleukin-8/MMP-9/HSFs
hindered
or
blockade
via
inhibitors
dismantling
NET-scaffold
DNase
I,
suggesting
candidate
therapeutic
targets
impairment
T2D.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(14), P. 11385 - 11385
Published: July 13, 2023
Mycobacterium
tuberculosis
complex
causes
(TB),
a
disease
that
pulmonary
inflammation
but
can
also
affect
other
tissues.
Despite
macrophages
having
defined
role
in
TB
immunopathogenesis,
innate
immune
cells,
such
as
neutrophils,
are
involved
this
process.
These
cells
have
high
phagocytic
ability
and
microbial-killing
machine
comprised
of
enzymes,
antimicrobial
peptides,
reactive
oxygen
species.
In
the
last
two
decades,
new
neutrophil
response,
extracellular
traps
(NETs),
has
been
intensely
researched.
NETs
comprise
DNA
associated
with
histones,
peptides.
structures
related
to
response
some
immuno-pathogenesis
mechanisms.
This
mini
review
highlights
how
they
be
helpful
diagnostic
tool
and/or
therapeutic
target.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
34(38)
Published: May 2, 2024
Abstract
Excessive
inflammation‐associated
unregulated
coagulation
leads
to
disseminated
intravascular
(DIC)
and
mortality
in
patients
with
sepsis
worldwide,
current
clinical
treatment
is
unsatisfactory.
Recent
studies
have
shown
that
circulating
cell‐free
DNA
(cfDNA)
neutrophil
extracellular
traps
(NETs),
act
as
potent
pro‐inflammation
pro‐coagulation
agents,
revealing
a
mechanistic
link
between
innate
immunity
coagulation.
After
establishing
definite
correlation
cfDNA
dysfunctional
inflammation
disorders
samples
from
sepsis,
novel
2D,
sheet‐like,
cationic
scavenger
using
polyamidoamine
(P‐G1)
antimicrobial
peptides
(AMPs)
covered
black
phosphorus
(BP)
nanosheets,
called
BP‐G1
AMP,
are
fabricated.
It
found
AMP
significantly
suppressed
bacterial
growth
ameliorated
the
systemic
inflammatory
response,
associated
disorder,
DIC,
resulting
improved
survival
cecal
ligation
puncture
(CLP)
mouse
model.
This
study
proposes
strategy
for
fabricating
multifunctional
nanosheets
can
ameliorate
during
treatment.
These
findings
demonstrate
importance
of
2D
nanostructures
construction
multivalent
anti‐bacterial
anti‐coagulation
nanoplatforms.
Nutrients,
Journal Year:
2023,
Volume and Issue:
15(15), P. 3364 - 3364
Published: July 28, 2023
Unripe
Rubus
occidentalis
(uRO)
contains
various
natural
polyphenols
with
beneficial
physiological
activities
and
is
particularly
rich
in
ellagic
acid
(EA).
EA
has
ameliorated
type
2
inflammation
airway
hyperresponsiveness
animal
models
of
eosinophilic
asthma.
metabolized
by
the
gut
microbiota
to
urolithin
A
(UA),
which
exhibits
anti-inflammatory
properties.
However,
it
remains
unclear
whether
uRO,
EA,
UA
reduce
inflammatory
responses
oxidative
stress
respiratory
epithelial
cells
neutrophils.
In
this
study,
was
induced
A549
(human
lung
cells)
dHL-60
(neutrophil-like
differentiated
from
human
promyelocytic
leukemia
HL-60
treated
concentrations
water
extract
uRO
(uRO-w),
UA.
uRO-w
suppressed
cytokine
chemokine
levels
reduced
expression
matrix
metalloproteinase-9
stimulated
IL-1β.
As
a
result
analyzing
mechanism
these
molecules
are
expressed,
found
that
uRO-w,
regulated
corticosteroid-sensitive
mitogen
activated
protein
kinase,
nuclear
factor
κB,
corticosteroid-insensitive
AKT.
addition,
significantly
reactive
oxygen
species
phorbol
12-myristate
13-acetate-stimulated
inhibited
neutrophil
extracellular
trap
formation.
Therefore,
our
results
suggest
potential
therapeutic
agents
for
preventing
treating
diseases.
