Altered Piwi-RNA Profiles of Peripheral Blood in Bone Metastasis of Non-Small Cell Lung Cancer DOI Creative Commons

Wenbin Fan,

Min Li,

Haoran Miao

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: July 5, 2023

Abstract Background PIWI-interacting RNAs (piRNA) are a novel class of non-coding RNAs. They involved in various pathophysiological activities. However, the expression profiles and functions piRNAs bone metastasis non-small cell lung cancer still largely unknown. Methods Blood samples were collected from patients with (NSCLC), NSCLC (BoM) healthy volunteers. To evaluate piRNA these samples, high-throughput microarray was performed. RStudio used for PCA analysis. Quantitative real-time polymerase chain reaction (qRT-PCR) to validate significantly dysregulated piRNAs. predict potential piRNAs, Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses The mRNA targets predicted using TargetScan miRanda software. interaction networks between their constructed Cytoscape Results A total 11026 differentially expressed identified, including 134 up-regulated 45 down-regulated (LCBM) vs. (LC) group 760 975 LCBM volunteers (Ctrl) P ≤0.05, fold change ≥1.0. Twenty-four genes statistically significant differences among all three groups selected be validated by qPCR. qRT-PCR validation confirmed reliability data. combining software analyses. Through GO KEGG analysis, found potentially BoM pathogenesis. In particular, pi-RNA might transcriptional misregulation osteoclast differentiation metastasis. Conclusions conclusion, we have identified that play important functional roles initiating progressing BoM. Bioinformatics suggested may an role tumorigenesis NSCLC, especially We piR-hsa-16644 is biomarkers therapeutic target BoM, piR-hsa-11510 strongly associated EGFR tyrosine kinase inhibitor resistance.

Language: Английский

Pharmacological and mechanistic aspects of quercetin in osteoporosis DOI Creative Commons
Tingting Deng,

Wenyu Ding,

Xi-Xue Lu

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 25, 2024

Osteoporosis (OP) is a bone disease associated with increasing age. Currently, the most common medications used to treat OP are anabolic agents, anti-resorptive and other mechanisms of action. However, many these have unfavorable adverse effects or not intended for long-term use, potentially exerting severe negative impact on patient’s life career placing heavy burden families society. There an urgent need find new drugs that can replace fewer effects. Quercetin (Que) flavonol in nature. Numerous studies examined therapeutic applications Que. comprehensive review anti-osteoporotic Que has yet been conducted. This aimed describe recent Que, including its biological, pharmacological, pharmacokinetic, toxicological properties. The outcomes demonstrated could enhance by osteoblast differentiation activity reducing osteoclast via pathways Wnt/β-catenin, BMP/SMAD/RUNX2, OPG/RANKL/RANK, ERK/JNK, oxidative stress, apoptosis, transcription factors. Thus, promising novel drug treatment OP.

Language: Английский

Citations

14
Exploring the therapeutic potential of quercetin in cancer treatment: Targeting long non-coding RNAs DOI
Farhad Sheikhnia, Ahmad Fazilat,

Vahid Rashidi

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 260, P. 155374 - 155374

Published: May 29, 2024

Language: Английский

Citations

8
Quercetin in Osteoporosis Treatment: A Comprehensive Review of Its Mechanisms and Therapeutic Potential DOI

Yanchen Feng,

Xue Dang,

Pan Zheng

et al.

Current Osteoporosis Reports, Journal Year: 2024, Volume and Issue: 22(3), P. 353 - 365

Published: April 23, 2024

Language: Английский

Citations

5
Regulatory Effects of Quercetin on Bone Homeostasis: Research Updates and Future Perspectives DOI
Yujiang Xi, Jiayan Shen,

Xiahuang Li

et al.

The American Journal of Chinese Medicine, Journal Year: 2023, Volume and Issue: 51(08), P. 2077 - 2094

Published: Jan. 1, 2023

The imbalance of bone homeostasis has become a major public medical problem amid the background an aging population, which is closely related to occurrence osteoporosis, osteoarthritis, and fractures. Presently, most drugs used in clinical treatment are bisphosphonates, calcitonin, estrogen receptor modulators, biological agents that inhibit resorption or parathyroid hormone analogs promote formation. However, there many adverse reactions. Therefore, it necessary explore potential drugs. Quercetin, as flavonol compound with various activities, widely distributed plants. Studies have found quercetin can regulate through multiple pathways targets. An in-depth exploration pharmacological mechanism great significance for development new This review discusses therapeutic mechanisms on homeostasis, such regulating expression long non-coding RNA, signaling metabolism, types programmed cell death, nutrients supply pathways, anti-oxidative stress, anti-inflammation, activation Sirtuins. We also summarize recent progress improving bioavailability propose some issues worth paying attention to, may help guide future research efforts.

Language: Английский

Citations

11
The role of LncRNAs and CircRNAs in osteoporosis: a focus on osteogenesis and osteoclastogenesis signaling pathways DOI Creative Commons
Suzan M. Ismail,

Mai A. Abd‐Elmawla,

Marwa I. Shabayek

et al.

Future Journal of Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 10(1)

Published: April 29, 2024

Abstract Background Osteoporosis is a crucial health concern interconnected with physical disabilities as well financial burdens. It arises from an imbalance between osteoblasts and osteoclasts, provoking the reduction of bone mass disturbances in structure high fracture risk. Considerable efforts were done to prevent mitigate this public issue. Nonetheless, further understanding etiopathology osteoporosis underlying genetic epigenetic pathways required. Main body Emerging evidence indicates that noncoding RNAs, including long RNAs (lncRNAs) circular (circRNAs), play roles regulators various pathological processes, osteoporosis. LncRNAs are RNA transcripts higher structural complexity developed owing their secondary tertiary structures, which allow them create different binding sites for other biomolecules, such DNA, RNA, proteins. Another class circRNAs, have covalently closed loop without 5′ cap 3′ polyA tail formed by back-splicing pre-mRNAs. Because structure, circRNAs largely stable, resistant RNA-degrading nucleases, possess substantially longer circulatory half-lives than linear RNAs. Interestingly, both lncRNAs serve competing endogenous sponging multiple miRNA interact RNA-binding proteins (RBPs), thereby controlling expression target genes. Several studies indicated altered these could influence many biological processes cells. Conclusion The current review provides opinions on role mechanisms affect osteoblastic osteoclastic activities. deep offers distinctive avenues innovative treatment strategies.

Language: Английский

Citations

4
Combination of dasatinib and quercetin promotes osteogenic differentiation and stemness maintenance of hPDLSCs via YAP/TAZ DOI Creative Commons

Yunge Qiu,

Yajun Zhao, Linglu Jia

et al.

Animal Cells and Systems, Journal Year: 2025, Volume and Issue: 29(1), P. 19 - 29

Published: March 12, 2025

Human periodontal ligament stem cells (hPDLSCs) are candidate seed for tissue regeneration. Enhancing the stemness maintenance and osteogenic differentiation potential of hPDLSCs is conducive to their role in The combination dasatinib quercetin, a type senolytic, has been reported affect cell senescence. However, whether it can regulate hPDLSCs, related mechanisms, remain unknown. present study analyzed optimal concentrations quercetin found that enhanced promoted expression stemness-related markers hPDLSCs. levels TAZ YAP were improved when incubated with quercetin. osteogenesis-promoting effects plus partly attenuated was knocked down, on suppressed inhibited. Taken together, promotes YAP/TAZ may be involved this process. This hold promise improving function

Language: Английский

Citations

0
Cancer chemoprevention: signaling pathways and strategic approaches DOI Creative Commons
Junling Ren,

Guangli Yan,

Le Yang

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)

Published: April 17, 2025

Abstract Although cancer chemopreventive agents have been confirmed to effectively protect high-risk populations from invasion or recurrence, only over ten drugs approved by the U.S. Food and Drug Administration. Therefore, screening potent is crucial reduce constantly increasing incidence mortality rate of cancer. Considering lengthy prevention process, an ideal agent should be nontoxic, inexpensive, oral. Natural compounds become a natural treasure reservoir for chemoprevention because their superior ease availability, cost-effectiveness, safety. The benefits as in various studies. In light this, present review intended fully delineate entire scope chemoprevention, primarily focuses on aspects based compounds, specifically focusing mechanism action prevention, discussing detail how they exert effects affecting classical signaling pathways, immune checkpoints, gut microbiome. We also introduce novel strategies summarize role improving chemotherapy regimens. Furthermore, we describe discovering anticancer with low abundance high activity, revealing broad prospects drug discovery chemoprevention. Moreover, associate precision medicine, discuss challenges encountered Finally, emphasize transformative potential advancing field ability more effective less toxic preventive options oncology.

Language: Английский

Citations

0
Quercetin-Based Biomaterials for Enhanced Bone Regeneration and Tissue Engineering DOI

Mohammad-Sadegh Lotfi,

Mohammad Sheibani, Majid Jafari‐Sabet

et al.

Tissue and Cell, Journal Year: 2024, Volume and Issue: 91, P. 102626 - 102626

Published: Nov. 20, 2024

Language: Английский

Citations

3
Sea buckthorn and its flavonoids isorhamnetin, quercetin, and kaempferol favorably influence bone and breast tissue health DOI Creative Commons
Monika Martiniaková,

Noémi Pénzes,

Roman Bíro

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 9, 2024

Bone tissue and breast are interrelated, as demonstrated by microcalcifications, cancer bone metastases, morphogenetic proteins, Wnt signaling. In addition, osteoblasts osteoclasts represent an important switch of tumor cell dormancy during metastasis. Damage to both types tissues mentioned above is highly prevalent, especially in postmenopausal women, manifests itself osteoporosis cancer. Sea buckthorn ( Elaeagnus rhamnoides L.), a botanical drug with high antioxidant, antitumor, anti-inflammatory, immunomodulatory, regenerative properties, has great therapeutic potential due the unique composition its bioactive metabolites. This review aimed summarize current knowledge from vitro vivo studies on effect sea buckthorn, well most widespread flavonoids isorhamnetin, quercetin, kaempferol, health. have revealed beneficial impacts aforementioned health (bone remodeling, mineralization, oxidative stress) (cancer proliferation, apoptosis, growth, metastatic behavior). documented their protective effects against disturbed microarchitecture reduced strength animal models osteoporosis, expansion properties xenograft models. any case, further research clinical trials needed carefully evaluate benefits flavonoids. Based available information, however, it can be concluded that these metabolites favorably affect

Language: Английский

Citations

0
MiR-378 exaggerates angiogenesis and bone erosion in collagen-induced arthritis mice by regulating endoplasmic reticulum stress DOI Creative Commons

Zhengmeng Yang,

Nan Hou,

Wenxiang Cheng

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(12)

Published: Dec. 18, 2024

Abstract Rheumatoid arthritis (RA) is a chronic autoimmune disorder marked by pain, inflammation, and discomfort in the synovial joints. It critical to understand pathological mechanisms of RA progression. MicroRNA-378 (miR-378) highly expressed synovium patients positively correlated with disease severity, but its function underlying remain poorly understood. In this study, miR-378 transgenic (miR-378 high ) mice were used construct collagen-induced (CIA) model for exploring role development. CIA showed accelerated development, as evidenced exaggerated joint swelling bone structural deformities. More severe endoplasmic reticulum (ER) stress consequent angiogenesis osteoclastogenesis also activated tissue calcaneus, respectively, group, suggesting that ER plays significant miR-378-mediated pathogenesis. Upon vitro induction, fibroblast-like synoviocytes (FLSs) isolated from higher expression level markers. The conditioned medium (CM) RA-FLSs stimulated more intensive osteoclastogenesis. stress-related protein Crebrf was identified downstream target miR-378. knockdown diminished promoting effect on stress, well activities. Tail vein injection anti-miR-378 lentivirus an established mouse shown ameliorate conclusion, amplified development osteoclastogenesis, thus indicating may be potential therapeutic treatment.

Language: Английский

Citations

0