Enteric Nervous System: Identification of a Novel Neuronal Sensory Network in the Duodenal Epithelium DOI Open Access
Víctor Salazar, Pura Bolaños, Jesús R. del Castillo

et al.

Journal of Histochemistry & Cytochemistry, Journal Year: 2023, Volume and Issue: 71(11), P. 601 - 630

Published: Oct. 4, 2023

The communication between the intestinal epithelium and enteric nervous system has been considered indirect. Mechanical or chemical stimuli activate enteroendocrine cells inducing hormone secretion, which act on sub-epithelial nerve ends, activating system. However, we identified an epithelial cell that expresses NKAIN4, a neuronal protein associated with β-subunit of Na+/K+-ATPase. This overexpresses Na+/K+-ATPase ouabain-insensitive Na+-ATPase, enzymes involved in active sodium transport. NKAIN4-positive also express markers as NeuN, acetylcholine-esterase, acetylcholine-transferase, α3- α7-subunits ACh receptors, glutamic-decarboxylase, serotonin-receptor-7, suggesting they are neurons. show polarized shape oval body, apical process finished knob-like terminal contact lumen, basal cilia body at base extension, axon-like soma projections connecting terminals, lymphoid nodules, glial cells, enterochromaffin forming network reaches surface. We showed, using retrograde labeling immunofluorescence, these receive afferent signals from Finally, demonstrated acetylcholine activates Ca2+ mobilization probably serotonin secretion cells. neurons would form part duodenal sensory for physiological noxious luminal stimuli.

Language: Английский

Nicotinic Acetylcholine Receptors in Glial Cells as Molecular Target for Parkinson’s Disease DOI Creative Commons
Érica Novaes Soares, Ana Carla dos Santos Costa,

Gabriel de Jesus Ferrolho

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(6), P. 474 - 474

Published: March 7, 2024

Parkinson’s disease (PD) is a progressive neurodegenerative characterized by resting tremor, bradykinesia, rigidity, and postural instability that also includes non-motor symptoms such as mood dysregulation. Dopamine (DA) the primary neurotransmitter involved in this disease, but cholinergic imbalance has been implicated. Current intervention PD focused on replenishing central DA, which provides remarkable temporary symptomatic relief does not address neuronal loss progression of disease. It well established nicotinic receptors (nAChRs) can regulate DA release nicotine itself may have neuroprotective effects. Recent studies identified nAChRs nonneuronal cell types, including glial cells, where they inflammatory responses. Given crucial role neuroinflammation dopaminergic degeneration involvement microglia astrocytes response, provide novel therapeutic target prevention and/or treatment PD. In review, following brief discussion PD, we focus cells and, specifically, their pathology treatment.

Language: Английский

Citations

11
Nicotinic acetylcholine receptors in cancer: Limitations and prospects DOI Creative Commons

Tanja Bele,

Tom Turk, Igor Križaj

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2023, Volume and Issue: 1870(1), P. 166875 - 166875

Published: Sept. 4, 2023

Nicotinic acetylcholine receptors (nAChRs) have long been considered to solely mediate neurotransmission. However, their widespread distribution in the human body suggests a more diverse physiological role. Additionally, expression of nAChRs is increased certain cancers, such as lung cancer, and has associated with cell proliferation, epithelial-to-mesenchymal transition, angiogenesis apoptosis prevention. Several compounds that interact these identified potential therapeutic agents. They tested drugs for treating nicotine addiction, alcoholism, depression, pain Alzheimer's disease. This review focuses on nAChR-mediated signalling presenting opportunities development innovative nAChR-based anticancer drugs. It displays differences each nAChR subunit between normal cancer cells selected types, highlighting possible involvement specific cases. Antagonists could complement existing therapies are summarised critically discussed. We hope this will stimulate further research role potentially leading therapies.

Language: Английский

Citations

12
Ionotropic and metabotropic responses by alpha 7 nicotinic acetylcholine receptors DOI Creative Commons
Patricia Sinclair, Nadine Kabbani

Pharmacological Research, Journal Year: 2023, Volume and Issue: 197, P. 106975 - 106975

Published: Oct. 29, 2023

Nicotinic acetylcholine receptors (nAChRs) belong to a superfamily of cys-loop characterized by the assembly five subunits into multi-protein channel complex. Ligand binding nAChRs activates rapid allosteric transitions receptor leading opening and ion flux in neuronal non-neuronal cell. Thus, while ionotropic properties are well recognized, less is known about ligand-mediated intracellular "metabotropic" signaling nAChRs. Studies neural non-neural cells confirm that metabotropic responses observable following ligand binding. In this review we summarize evidence on existence homopentameric α7 various cell types. We explore how coordinated calcium entry through release from nearby stores gives rise important for modulation cytoskeletal motility growth. Amino acid residues for, protein within human nAChR support engagement including heterotrimeric G proteins immune cells. Understanding dual likely essential advancing drug development other mechanistic treatments disease.

Language: Английский

Citations

12
Role of Glial Cells and Receptors in Schizophrenia Pathogenesis DOI
Yousef Tizabi, Marta C. Antonelli,

Daniela Tizabi

et al.

Neurochemical Research, Journal Year: 2025, Volume and Issue: 50(2)

Published: Jan. 27, 2025

Language: Английский

Citations

0
Perspectives on the α5 nicotinic acetylcholine receptor in lung cancer progression DOI Creative Commons

Jiaying Cai,

Jingting Wang,

Z. Wang

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: March 12, 2025

Nicotinic acetylcholine receptors (nAChRs) are widely expressed in a variety of cell types and involved multiple physiological regulatory mechanisms cells, tissues systems. Increasing evidence suggests that the α5 nicotinic receptor (α5-nAChR), encoded by CHRNA5 gene, is one key mediator lung cancer development immune responses. Several studies have shown it regulator stimulates processes via various signaling pathways, including STAT3 cancer. In addition, α5-nAChR has profound effect on response through immune-related factor pathways. this review, we focus perspectives progression, which indicates targeting could provide novel anticancer therapy strategies for

Language: Английский

Citations

0
Choline Levels in the Pregenual Anterior Cingulate Cortex Associated with Unpleasant Pain Experience and Anxiety DOI Creative Commons
Lina Hudhud, Jón Hauksson, Michael Haney

et al.

NeuroImage, Journal Year: 2025, Volume and Issue: unknown, P. 121153 - 121153

Published: March 1, 2025

In vivo proton magnetic resonance spectroscopy is a non-invasive technique used to measure biochemical molecules such as choline, glutamate, glutamine, and γ-Aminobutyric acid (GABA), many of which are relevant anxiety pain. However, the relationship between these neurotransmitters/metabolites their implications for subjective experience pain not yet fully understood. The objective this cross-sectional study was investigate association ratings with levels total glutamate GABA in brain regions known be involved emotional pain, specifically pregenual anterior cingulate cortex (pgACC) dorsal (dACC). were measured using GABA-edited Mescher-Garwood PRESS measurements, OFF-sequence measurements choline (tCho) Glx (combined + glutamine). signal our analysis included glycerophosphocholine (GPC) phosphocholine (PC), consistent standard practices MRS studies. This approach ensures robust estimation tCho concentrations across participants. collected data from 38 participants (17 males 21 females). revealed significant correlation before standardized provocation rated unpleasantness during provocation. correlated negatively parameters pgACC. A linear regression indicated that pgACC has negative perceived when controlling age, depressive symptoms, alcohol tobacco intake. We also found sex significantly moderates unpleasantness. suggests an essential precursor acetylcholine, associated These may influence how contribute affective experiences by modulating balance excitatory inhibitory signals. future research needed identify mechanisms involved. Furthermore, indicates factor relationship, lower being higher females but males. highlights significance addressing biological better understand different responses treatments facilitate development more effective interventions future.

Language: Английский

Citations

0
Synthesis and Characterization of Chitosan Acetylcholine Nanoparticles for Neural Disorders Associated with Cancer Treatment DOI
Mamilla Ravi Sankar, R. Karthikeyan,

S. Vigneshkumar

et al.

Journal of Inorganic and Organometallic Polymers and Materials, Journal Year: 2023, Volume and Issue: 33(8), P. 2465 - 2484

Published: May 13, 2023

Language: Английский

Citations

6
Molecular Targets for Cannabinoids in Natural Killer Cells: Do They Modulate the Antitumor Activity? DOI Creative Commons
Miguel Olivas-Aguirre,

Cecilia Gutiérrez-Iñiguez,

Igor Pottosin

et al.

Receptors, Journal Year: 2024, Volume and Issue: 3(2), P. 122 - 144

Published: March 25, 2024

Recent research has emphasized the potential of natural and synthetic cannabinoids as anticancer agents. Yet it remains unclear whether in which sense affect activity NK cells, an important branch immunity. Similar uncertainty exists regarding cells-based immunotherapy. Here we presented overview multiple cannabinoid targets canonical (mainly CB2) non-canonical receptors, ion channels, transporters, enzymes, expressed along with underlying molecular mechanisms. Through them, can viability, proliferation, migration, cytokine production, overall cells. Respective holistic studies are limited, and, mostly, phenomenological, not linking observed effects certain targets. Another problem existing is lack standardisation, so that diverse at variable concentrations ways administration applied, often, instead purified whole lymphocyte population used. Therefore, there urgent need for more focused, systemic, in-depth impact toolkit on cell function, to critically address compatibility synergies between utilization realm interventions.

Language: Английский

Citations

2
Silencing LY6D Expression Inhibits Colon Cancer in Xenograft Mice and Regulates Colon Cancer Stem Cells’ Proliferation, Stemness, Invasion, and Apoptosis via the MAPK Pathway DOI Creative Commons

Jinyue Duan,

Yi Wang, Yuanyuan Chen

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(23), P. 7776 - 7776

Published: Nov. 25, 2023

This study explored the role of lymphocyte antigen 6 family member D (LY6D) in colon cancer stem cells’ (CCSCs) proliferation and invasion. LY6D was knocked down using siRNA, down-regulation verified Western blotting. After knockdown, CCSCs’ proliferation, stemness, invasion were suppressed, whereas apoptosis increased. Gene Ontology (GO) enrichment analysis revealed that differentially expressed genes (DEGs) between siLY6D negative control groups significantly enriched cell–substrate adherens junction, focal adhesion, junction terms. Meanwhile, Kyoto Encyclopedia Genes Genomes (KEGG) DEGs MAPK pathway. In addition, blotting results showed pBRAF pERK1/2, cascade kinases pathway, down-regulated after knockdown. nude mice xenograft experiments treatment decreased tumor sizes weights improved tumor-bearing survival rates compared with group. conclusion, these findings indicate LY6D, which is highly CCSCs, a key factor involved growth development might be potential marker therapeutic target for cancer.

Language: Английский

Citations

4
Nicotinic Acetylcholine Receptors in Glial Cells as Molecular Target for Parkinson’s Disease DOI Open Access
Érica Novaes Soares, Ana Carla dos Santos Costa,

Gabriel de Jesus Ferrolho

et al.

Published: March 4, 2024

Parkinson’s disease (PD) is a progressive neurodegenerative characterized by resting tremor, bradykinesia, rigidity, postural instability, that also includes non-motor symptoms such as mood dysregulation. Dopamine (DA) the primary neurotransmitter involved in this disease, but cholinergic imbalance has been implicated. Current intervention PD focused on replenishing central DA, which provides remarkable temporary symptomatic relief does not address neuronal loss and progression of disease. It well established nicotinic receptors (nAChRs) can regulate DA release nicotine itself may have neuroprotective effects. Recent studies identified nAChRs nonneuronal cell types including glial cells, where they inflammatory responses. Given crucial role neuroinflammation dopaminergic degeneration, involvement microglia astrocytes response, provide novel therapeutic target prevention and/or treatment PD. In review, following brief discussion PD, we focus cells specifically their pathology treatment.

Language: Английский

Citations

1