Frontiers in Genetics,
Journal Year:
2025,
Volume and Issue:
16
Published: May 15, 2025
Introduction
Atractylodes
lancea
(Thunb.)
DC.,
a
widely
utilized
herb
in
traditional
Chinese
medicine,
contains
sesquiterpenoids
and
polyacetylenes
as
its
primary
bioactive
components.
The
WRKY
gene
family
plays
critical
role
regulating
various
biological
processes
plants.
However,
the
molecular
mechanism
underlying
AlWRKY
regulation
of
terpenoid
synthesis
unclear.
Methods
members
were
identified
through
bioinformatics
approaches.
Gene
structures,
motifs,
phylogenetic
relationships
analyzed.
Subsequently,
their
expression
profiles
across
different
geographical
origins
investigated
using
transcriptome
data.
Furthermore,
preliminary
validation
was
performed
via
methyl
jasmonate
treatment
docking,
with
focus
on
AlWRKY20
AlWRKY37
genes.
Results
In
this
study,
65
genes
conserved
domains
A.
classified
into
three
groups:
Group
I
(17
members),
II
(33
III
(15
members).
Tissue-specific
profiling
revealed
five
rhizome-enriched
(
AlWRKY13
,
AlWRKY21
AlWRKY49
)
highly
expressed
Hubei
accessions
compared
to
Jiangsu
accessions,
co-expression
analysis
demonstrated
strong
correlation
16
AlTPS
Quantitative
PCR
(qPCR)
confirmed
specific
upregulation
rhizomes,
consistent
accumulation
patterns
sesquiterpenes
(hinesol,
γ-eudesmol,
elemol).
Methyl
(MeJA)
induction
experiments
(12
h)
coordinated
AlTPS70
AlTPS71
concomitant
significantly
increased
cis-β-farnesene
α-curcumene
content.
Molecular
docking
binding
affinities
AlTPS70/AlTPS71
promoter
promoter.
Subcellular
localization
that
both
are
localized
nucleus.
These
results
suggest
likely
function
regulators
sesquiterpene
biosynthesis,
positively
production
modulation.
Discussion
This
study
lays
groundwork
for
further
exploration
mechanisms
functional
transcription
factors
.
Plants,
Journal Year:
2024,
Volume and Issue:
13(5), P. 626 - 626
Published: Feb. 24, 2024
Plant-plant
interactions
are
a
central
driver
for
plant
coexistence
and
community
assembly.
Chemically
mediated
plant-plant
represented
by
allelopathy
allelobiosis.
Both
allelobiosis
achieved
through
specialized
metabolites
(allelochemicals
or
signaling
chemicals)
produced
released
from
neighboring
plants.
Allelopathy
exerts
mostly
negative
effects
on
the
establishment
growth
of
plants
allelochemicals,
while
provides
neighbor
detection
identity
recognition
chemicals.
Therefore,
can
chemically
affect
performance
that
frequently
occur
in
intra-specific
inter-specific
interactions.
two
probably
inseparable
processes
together
chemical
Here,
we
comprehensively
review
interactions,
including
allelochemicals
their
application
sustainable
agriculture
forestry,
recognition,
root-soil
plant-soil
feedback,
biosynthesis
molecular
mechanisms
Altogether,
these
efforts
provide
recent
advancements
wide
field
allelobiosis,
new
insights
into
New Phytologist,
Journal Year:
2024,
Volume and Issue:
244(3), P. 914 - 933
Published: Sept. 2, 2024
Summary
Trichomes
are
specialized
epidermal
outgrowths
covering
the
aerial
parts
of
most
terrestrial
plants.
There
is
a
large
species
variability
in
occurrence
different
types
trichomes
such
that
molecular
regulatory
mechanism
underlying
formation
and
biological
function
plant
remain
unexplored.
Here,
we
used
Chrysanthemum
morifolium
as
model
to
explore
network
trichome
terpenoid
synthesis
unravel
physical
chemical
roles
constitutive
defense
against
herbivore
feeding.
By
analyzing
trichome‐related
genes
from
transcriptome
database
trichomes‐removed
leaves
intact
leaves,
identified
CmMYC2
positively
regulate
both
development
T
‐shaped
glandular
well
content
terpenoids
stored
trichomes.
Furthermore,
found
role
terpene
was
mediated
by
interaction
with
CmMYBML1.
Our
results
reveal
sophisticated
wherein
CmMYC2–CmMYBML1
feedback
inhibition
loop
regulates
(non‐glandular
glandular)
biosynthesis,
collectively
contributing
enhanced
resistance
Spodoptera
litura
larvae
findings
provide
new
insights
into
novel
which
synchronously
density
for
herbivory.
BMC Plant Biology,
Journal Year:
2024,
Volume and Issue:
24(1)
Published: Feb. 2, 2024
Abstract
Background
Cinnamomum
cassia
Presl,
classified
in
the
Lauraceae
family,
is
widely
used
as
a
spice,
but
also
medicine,
cosmetics,
and
food.
Aroma
an
important
factor
affecting
medicinal
flavoring
properties
of
C.
,
mainly
determined
by
volatile
organic
compounds
(VOCs);
however,
little
known
about
composition
aromatic
VOCs
their
potential
molecular
regulatory
mechanisms.
Here,
integrated
transcriptomic
metabolomic
analyses
were
employed
to
provide
insights
into
formation
regularity
bark
at
five
different
harvesting
times.
Results
The
thickness
oil
content
significantly
increased
along
with
development
bark.
A
total
724
differentially
accumulated
volatiles
(DAVs)
identified
samples,
most
which
terpenoids.
Venn
analysis
top
100
each
period
showed
that
twenty-eight
abundant
VOC,
cinnamaldehyde,
peaked
120
months
after
planting
(MAP)
dominated
aroma
qualities.
Five
terpenoids,
α-copaene,
β-bourbonene,
α-cubebene,
α-funebrene,
δ-cadinene,
240
MAP
could
be
creating
’s
characteristic
aroma.
list
43,412
expressed
genes
(DEGs)
involved
biosynthetic
pathways
identified,
including
phenylpropanoids,
mevalonic
acid
(MVA)
methylerythritol
phosphate
(MEP).
gene-metabolite
network
for
terpenoid
phenylpropanoid
metabolism
was
constructed
show
key
candidate
structural
transcription
factors
biosynthesis
terpenoids
phenylpropanoids.
Conclusions
results
our
research
revealed
changes
stages,
differentiated
components
cinnamon,
illuminated
mechanism
formation.
These
foundational
will
technical
guidance
quality
breeding
.
