Medicine International,
Journal Year:
2024,
Volume and Issue:
5(2)
Published: Dec. 20, 2024
The
limitations
of
two‑dimensional
(2D)
models
in
cancer
research
have
hindered
progress
fully
understanding
the
complexities
drug
resistance
and
therapeutic
failures.
However,
three‑dimensional
(3D)
provide
a
more
accurate
representation
in
vivo
environments,
capturing
critical
cellular
interactions
dynamics
that
are
essential
evaluating
efficacy
toxicity
tyrosine
kinase
inhibitors
(TKIs).
These
advanced
enable
researchers
to
explore
mechanisms
with
greater
precision,
optimizing
treatment
strategies
improving
predictive
accuracy
clinical
outcomes.
By
leveraging
3D
models,
it
will
be
possible
deepen
current
TKIs
drive
forward
innovations
treatment.
present
review
discusses
2D
transformative
impact
on
oncology
research,
highlighting
their
roles
addressing
challenges
systems
advancing
TKI
studies.
EJNMMI Physics,
Journal Year:
2024,
Volume and Issue:
11(1)
Published: Nov. 13, 2024
177Lu-based
radiopharmaceuticals
(RPs)
are
the
most
used
for
targeted
radionuclide
therapy
(TRT)
due
to
their
good
response
rates.
However,
worldwide
availability
of
177Lu
is
limited.
161Tb
represents
a
potential
alternative
TRT,
as
it
emits
photons
SPECT
imaging,
β−-particles
therapy,
and
also
releases
significant
yield
internal
conversion
(IE)
Auger
electrons
(AE).
This
research
aimed
evaluate
cell
dosimetry
with
MIRDcell
code
considering
realistic
localization
three
161Tb-
177Lu-somatostatin
(SST)
analogs
in
different
subcellular
regions
reported
literature,
various
cluster
sizes
(25–1000
µm
radius)
percentage
labeled
cells.
Experimental
values
α-
β-survival
coefficients
determined
by
external
beam
photon
irradiation
were
estimate
survival
fraction
(SF)
AR42J
pancreatic
clusters
micrometastases.
The
RPs
same
within
cells,
resulted
only
slight
variations
dose
absorbed
nuclei
(ADN)
cells
no
differences
observed
either
unlabeled
or
SF.
ADN
(MDLC)
produced
161Tb-RPs
from
2.8–3.7
times
higher
than
those
delivered
177Lu-RPs
radius
lower
0.1
mm
10%
amount
energy
emitted
161Tb-disintegration
form
IE
AE.
161Tb-RPs/177Lu-RPs
MDLC
ratio
decreased
below
1.6
larger
(0.5–1
mm)
>
40%
significantly
cross-irradiation
contribution.
Using
fixed
number
disintegrations,
SFs
177Lu-RPs,
but
when
was
SF
between
177Lu-
161Tb-RPs,
except
smallest
sizes.
Despite
emissions
AE
exerted
negligible
influence
on
ADN.
damage
could
be
achieved
using
smaller
quantities
thus
making
suitable
TRT.
Journal of Biomedical Materials Research Part A,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 16, 2024
ABSTRACT
Multidrug
resistance
remains
one
of
the
major
challenges
in
breast
cancer
research,
often
leading
to
treatment
failure.
To
better
understand
this
mechanism,
sophisticated
three‐dimensional
(3D)
tumor
models
are
necessary,
as
they
offer
several
advantages
over
traditional
bidimensional
(2D)
cultures.
In
study,
poly‐
l
‐lactic‐acid
porous
scaffolds
were
produced
using
a
thermally
induced
phase
separation
technique
and
employed
3D
for
cell
lines:
MDA‐MB‐231,
MCF‐7,
its
multidrug‐resistant
variant,
MCF‐7R.
The
MTS
assay
was
used
compare
growth
inhibition
following
doxorubicin
2D
3D.
Remarkably,
IC
50
values
increased
cultures
compared
2D:
MDA‐MB‐231
(445
vs.
54.5
ng/mL),
MCF‐7
(7.45
0.75
μg/mL),
MCF‐7R
(165
39
μg/mL).
MCF‐7R,
which
usually
shows
greater
2D,
demonstrated
even
higher
fact,
not
reached
within
3
days
with
other
models,
but
only
after
6
days.
Cellular
morphology
also
played
crucial
role.
When
treated
concentrations
than
,
cells
lost
their
physiological
clustered
structure,
while
resistant
variant
exhibited
disrupted
layers.
All
lines
showed
chemoresistance,
suggesting
more
biomimetic
spatial
architecture.
Our
work
bridges
gap
between
monolayer
animal
highlighting
potential
polymeric
research.
Understanding
the
biological
activity
of
molecules
is
pivotal
for
drug
discovery
and
development.
In
field
pharmaceutical
development
safety,
there
a
critical
need
accurate
reliable
testing
methodologies.
These
methodologies
are
essential
evaluating
purity
performance
compounds
identifying
potential
adverse
effects.
Here,
we
discuss
various
their
applications.
We
examine
cell
culture
methodologies,
which
involve
drugs
on
cultured
human
or
animal
cells.
also
explore
live
animals
to
assess
safety
efficacy.
Additionally,
emerging
3D
use
three-dimensional
cultures
better
mimic
complex
microenvironments
found
in
vivo.
Furthermore,
address
ethical
considerations
surrounding
propose
solutions
mitigate
these
concerns.
This
comprehensive
review
paper
offers
an
extensive
exploration
within
context
culture,
shedding
light
methods
practices
employed
this
domain
biomedical
research
it
will
serve
as
valuable
resource
scientists,
clinicians,
researchers,
facilitating
deeper
understanding
realm
crucial
role
advancing
science
therapeutic
Medicine International,
Journal Year:
2024,
Volume and Issue:
5(2)
Published: Dec. 20, 2024
The
limitations
of
two‑dimensional
(2D)
models
in
cancer
research
have
hindered
progress
fully
understanding
the
complexities
drug
resistance
and
therapeutic
failures.
However,
three‑dimensional
(3D)
provide
a
more
accurate
representation
in
vivo
environments,
capturing
critical
cellular
interactions
dynamics
that
are
essential
evaluating
efficacy
toxicity
tyrosine
kinase
inhibitors
(TKIs).
These
advanced
enable
researchers
to
explore
mechanisms
with
greater
precision,
optimizing
treatment
strategies
improving
predictive
accuracy
clinical
outcomes.
By
leveraging
3D
models,
it
will
be
possible
deepen
current
TKIs
drive
forward
innovations
treatment.
present
review
discusses
2D
transformative
impact
on
oncology
research,
highlighting
their
roles
addressing
challenges
systems
advancing
TKI
studies.
