TRIM44 Promotes Rabies Virus Replication by Autophagy-Dependent Mechanism DOI Open Access

Hongling He,

Ting Cai,

Qiaozhu Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4616 - 4616

Published: April 23, 2024

Tripartite motif (TRIM) proteins are a multifunctional E3 ubiquitin ligase family that participates in various cellular processes. Recent studies have shown TRIM play important roles regulating host-virus interactions through specific pathways, but their involvement response to rabies virus (RABV) infection remains poorly understood. Here, we identified several upregulated mouse neuroblastoma cells (NA) after with the using RNA-seq sequencing. Among them, TRIM44 was found regulate RABV replication. This is supported by observations downregulation of inhibits replication, while overexpression promotes Mechanistically, TRIM44-induced replication brought about activating autophagy, as inhibition autophagy 3-MA attenuates Additionally, rapamycin reverses TRIM44-knockdown-induced decrease LC3B expression and autophagosome formation well The results suggest an autophagy-dependent mechanism. Our work identifies key host factor for targeting may represent effective therapeutic strategy.

Language: Английский

Human Rabies Treatment—From Palliation to Promise DOI Creative Commons
M. Lacy, Nonthapan Phasuk, Stephen J. Scholand

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(1), P. 160 - 160

Published: Jan. 22, 2024

Rabies encephalitis has plagued humankind for thousands of years. In developed countries, access to preventive care, both pre-exposure and post-exposure, significantly reduced the burden suffering disease. However, around world, rabies remains a neglected tropical disease, largely due uncontrolled dog rabies, tens perish each year. Currently, standard care management is palliation. Heroic attempts treat human patients over last few decades have yielded glimpses into our understanding pathophysiology, opening door development new antiviral therapies modalities treatment. Researchers continue investigate compounds approaches therapy, yet there remain real challenges given complexity We explore review some promising on horizon in pursuit salvage treatment rabies.

Language: Английский

Citations

6
TRIM21 Promotes Oxidative Stress and Ferroptosis through the SQSTM1-NRF2-KEAP1 Axis to Increase the Titers of H5N1 Highly Pathogenic Avian Influenza Virus DOI Open Access
Yifan Wei,

Yongxia Gu,

Ziwei Zhou

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3315 - 3315

Published: March 14, 2024

Tripartite motif-containing protein 21 (TRIM21) is involved in signal transduction and antiviral responses through the ubiquitination of targets. TRIM21 was reported to be related imbalance host cell homeostasis caused by viral infection. Our studies indicated that H5N1 highly pathogenic avian influenza virus (HPAIV) infection up-regulated expression A549 cells. Western blot qPCR results showed knockdown alleviated oxidative stress ferroptosis induced HPAIV promoted activation antioxidant pathways. Co-IP regulating SQSTM1-NRF2-KEAP1 axis increasing SQSTM1 K63-linked polyubiquitination under condition In addition, attenuated inhibitory effect NAC on titers enhanced promoting agonist Erastin titers. findings provide new insight into role

Language: Английский

Citations

5
Role of cytokines in rabies virus infection DOI

Sai Praneet Nalla,

Neetesh Mandal,

Rajaneesh Anupam

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 247 - 257

Published: Jan. 1, 2025

Language: Английский

Citations

0
Selective Autophagy Mediated by Protein Ubiquitination in Major Prevalent Zoonoses DOI Creative Commons

Chi Meng,

Fengyuan Jiao,

Gengxu Zhou

et al.

Transboundary and Emerging Diseases, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Zoonotic diseases not only cause great harm to animal health but also involve the development of husbandry, which in turn endangers human life and public safety. Protein ubiquitination autophagy are important ways for body degrade invading pathogens, correspond ubiquitin (Ub)‐proteasome system (UPS) autophagic lysosomal pathway (ALP), respectively, play an role occurrence diseases. For UPS, substrate is delivered 26S proteasome via a cascade subsequently degraded removed. ALP, encapsulated form autophagosomes, fuse with lysosomes autophagolysosomes, eventually cleared. However, variety zoonotic pathogens can interfere protein process promote self‐replication survival, resist host immune defense. This article reviews mechanisms by multiple degradation pathways, providing new perspective treatment prevention

Language: Английский

Citations

0
Friend or foe of tripartite motif-containing protein 21 in cardiovascular disease: A review DOI

Xiangmei Gong,

Lei Xu, Pengcheng Cai

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142682 - 142682

Published: March 1, 2025

Language: Английский

Citations

0
TRIM44 Promotes Rabies Virus Replication by Autophagy-Dependent Mechanism DOI Open Access

Hongling He,

Ting Cai,

Qiaozhu Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4616 - 4616

Published: April 23, 2024

Tripartite motif (TRIM) proteins are a multifunctional E3 ubiquitin ligase family that participates in various cellular processes. Recent studies have shown TRIM play important roles regulating host-virus interactions through specific pathways, but their involvement response to rabies virus (RABV) infection remains poorly understood. Here, we identified several upregulated mouse neuroblastoma cells (NA) after with the using RNA-seq sequencing. Among them, TRIM44 was found regulate RABV replication. This is supported by observations downregulation of inhibits replication, while overexpression promotes Mechanistically, TRIM44-induced replication brought about activating autophagy, as inhibition autophagy 3-MA attenuates Additionally, rapamycin reverses TRIM44-knockdown-induced decrease LC3B expression and autophagosome formation well The results suggest an autophagy-dependent mechanism. Our work identifies key host factor for targeting may represent effective therapeutic strategy.

Language: Английский

Citations

2