Critical Reviews in Microbiology,
Journal Year:
2024,
Volume and Issue:
unknown, P. 1 - 20
Published: Sept. 3, 2024
Pseudomonas
aeruginosa
(PA),
an
opportunistic
human
pathogen
that
is
frequently
linked
with
chronic
infections
in
immunocompromised
individuals,
also
metabolically
versatile,
and
thrives
diverse
environments.
Additionally,
studies
report
PA
can
interact
other
microorganisms,
such
as
bacteria,
fungi,
producing
unique
metabolites
modulate
the
host
immune
response,
contribute
to
disease
pathogenesis.
This
review
summarizes
current
knowledge
related
metabolic
interactions
of
microorganisms
(Staphylococcus,
Acinetobacter,
Klebsiella,
Enterococcus,
Candida)
hosts,
importance
these
a
polymicrobial
context.
Further,
we
highlight
potential
applications
studying
toward
designing
better
diagnostic
tools,
therapeutic
strategies
prevent,
treat
caused
by
this
pathogen.
Bacteria,
Journal Year:
2024,
Volume and Issue:
3(3), P. 118 - 135
Published: June 24, 2024
Biofilms
are
accumulations
of
microorganisms
in
an
extracellular
polymeric
substance
matrix
which
composed
polysaccharides,
proteins,
lipids,
and
nucleic
acids.
Many
bacteria
can
switch
between
a
planktonic
form
biofilm
form.
The
have
relatively
high
cell
growth
reproduction
rates
reduced
likelihood
survival
but
adapt
to
occupy
new
habitats.
state
appears
be
natural
predominant
bacteria.
need
for
the
formation
bacterial
is
that
it
enhances
tolerance
harsh
environmental
conditions,
thereby
allowing
avoid
being
washed
away
by
water
flow
or
bloodstream
simply
attaching
surface
tissue,
EPS
protects
cells,
deeper
layers,
against
antimicrobial
agents,
probably
limiting
diffusion
these
agents.
Biofilm
steps
initial
contact/attachment
surface,
followed
micro-colony
formation,
maturation
architecture
biofilm,
finally
detachment/dispersion
biofilm.
Once
formed,
restricts
mobility
increases
density.
Secretions
autoinducers
into
environment
critical
cross-signaling
This
cross-talk
called
quorum
sensing
(QS).
Quorum
cell–cell
communication
mechanism
allows
specific
processes
controlled,
such
as
virulence
factor
expression.
Bacterial
signaling
mainly
consists
acyl-homoserine
lactones
(produced
Gram-negatives),
autoinducing
peptides
Gram-positives),
autoinducer-2
both
Gram-negatives
Gram-positives).
Therefore,
this
review
aimed
at
how
biofilms
work
formed.
Medical Sciences,
Journal Year:
2025,
Volume and Issue:
13(1), P. 6 - 6
Published: Jan. 3, 2025
Pseudomonas
aeruginosa
is
a
clinically
significant
opportunistic
pathogen,
renowned
for
its
ability
to
acquire
and
develop
diverse
mechanisms
of
antibiotic
resistance.
This
study
examines
the
resistance,
virulence,
regulatory
in
extensively
drug-resistant
clinical
strains
P.
aeruginosa.
Antibiotic
susceptibility
was
assessed
using
Minimum
Inhibitory
Concentration
(MIC)
method,
whole-genome
sequencing
(WGS)
performed
on
Illumina
NovaSeq
platform.
The
analysis
demonstrated
higher
prevalence
virulence
genes
compared
resistance
genes.
Key
factors
identified
included
secretion
systems,
motility,
adhesion,
biofilm
formation.
Resistance
observed
comprised
efflux
pumps
beta-lactamases,
while
systems
involved
two-component
transcriptional
regulators,
sigma
factors.
Additionally,
phenotypic
profiles
were
found
correlate
with
through
genotypic
analysis.
underscores
analyzed,
highlighting
urgent
need
alternative
strategies
address
infections
caused
by
bacteria.
Journal of Applied Microbiology,
Journal Year:
2025,
Volume and Issue:
136(3)
Published: Feb. 19, 2025
Abstract
Aim
Pseudomonas
aeruginosa
employs
the
quorum
sensing
(QS)
system,
a
sophisticated
cell-to-cell
communication
mechanism,
to
modulate
synthesis
and
secretion
of
range
virulence
factors,
which
contribute
establishment
acute
or
chronic
infections
in
hosts.
This
study
seeks
attenuate
P.
by
inhibiting
QS
thereby
reducing
its
pathogenicity
as
promising
alternative
traditional
antibiotics.
Methods
results
Two
compounds
with
an
amino-substituted
diphenyl
fumaramide
core,
N1-(4-bromophenyl)-N4-(4′-oxo-3′,4′-dihydro-1′H-spiro
[cyclopentane-1,2′-quinazolin]-6′-yl)
(10D)
N1-(3-chloro-4-fluorophenyl)-N4-(4-oxo-3,4,4′,5′-tetrahydro-1H,2′H-spiro
[quinazoline-2,3′-thiophen]-6-yl)
(12A),
were
identified
through
in-silico
screening.
The
inhibitory
potential
both
was
explored
vitro
vivo.
In
experiments,
neither
compound
exhibited
bactericidal
effects
but
significantly
inhibited
production
QS-regulated
extracellular
protease
pyocyanin.
Quantitative
PCR
analysis
revealed
that
QS-activated
genes
downstream
transcriptionally
suppressed
10D
12A.
Molecular
docking
molecular
dynamics
simulations
predicted
stable
interactions
between
these
key
regulators
LasR
PqsR.
When
combined
polymyxin
B,
kanamycin,
levofloxacin,
12A
synergistic
antibacterial
activity.
Furthermore,
improved
survival
mice
challenged
effectively
reduced
bacterial
load
lungs.
Conclusion
indicates
possess
considerable
potential,
attenuating
aeruginosa.
Moreover,
offers
structural
insights
methodological
guidance
for
advancement
anti-virulence
drug
development.
BMC Microbiology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: Feb. 20, 2025
Pseudomonas
aeruginosa
is
notorious
for
its
complex
virulence
system
and
rapid
adaptive
drug
resistance.
This
study
aimed
to
compare
the
prevalence
genotype
distribution
of
genes
in
multidrug-sensitive
multidrug-resistant
clinical
strains
aeruginosa.
It
possible
better
understand
genetic
characteristics
carry
out
effective
treatment
prevention
measures.
The
phzS,
aprA,
plcH,
toxA,
pilA
exoU
were
detected
amongst
184
strains,
whose
cytotoxicity
biofilm
formation
ability
evaluated
as
well.
Phenotypic
screening
susceptibility
was
conducted
by
standard
antimicrobial
test
interpreted
according
standards
established
CLSI.
A
total
94
90
isolates
included
this
study.
Statistically
significant
relationship
observed
frequency
toxA
(p
=
0.002)
plcH
0.001)
between
strains.
Moreover,
thirteen
genotypes
seven
them
groups.
There
statistically
correlation
found
presence
IV
VII
two
subgroups.
Additionally,
that
III
exhibited
most
obvious
cytotoxicity,
showed
cytotoxicity.
strong
biofilm-formation
accounted
a
relatively
high
proportion
VI
These
could
form
abundant
varieties,
overall
number
greater
multi-sensitive
In
addition,
particular
characteristically
distributed
different
abilities.
