Embedding the Entrepreneurial Mindset into Undergraduate Bioengineering Courses: Two Instructional Laboratory Case Studies DOI
Caroline Cvetkovic,

Keilin Jahnke,

Sarah Lindley

et al.

Published: Aug. 4, 2024

Abstract Experimental laboratory courses are foundational to undergraduate instruction in various engineering disciplines. Traditionally, students demonstrate competence curricula through protocol-based experimentation as well assignments such lab reports, but generally do not gain experience open-ended design or entrepreneurial innovation – skills which may be highly valued by future employers graduate schools, and even upper-level project-based capstone design. Biofabrication Lab is an elective course offering hands-on advanced cell culture, computer aided design, additive manufacturing, prototyping related translational bioengineering. Quantitative Physiology a required third-year bioengineering conduct experiments measure model human physiological systems, while gaining data analysis presentation. To expand the breadth of knowledge gained students, project with new learning objectives was incorporated into both courses. Through expert lectures robust project, were introduced technology commercialization mindset, that aid development career-ready innovative engineers. Students applied these concepts laboratory-based participating product pitch competition justify value their panel experts field who exemplified potential investors. outcomes, we assessed students' self-reported expertise components disciplines surveys administered at multiple points throughout modules gathered anonymous feedback end-of-semester evaluations. In this paper, detail projects courses, resource introduce basics mindset technical settings, highlight observed outcomes. Finally, providing evidence module's implementation two distinct vary class size, topic, scope, organization, aim provide suggestions encouragement for translation module variety STEM

Language: Английский

Recent Research Trends in Neuroinflammatory and Neurodegenerative Disorders DOI Creative Commons
Jessica R. Cohen,

Annette Mathew,

Kirk D. Dourvetakis

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(6), P. 511 - 511

Published: March 14, 2024

Neuroinflammatory and neurodegenerative disorders including Alzheimer’s disease (AD), Parkinson’s (PD), traumatic brain injury (TBI) Amyotrophic lateral sclerosis (ALS) are chronic major health disorders. The exact mechanism of the neuroimmune dysfunctions these pathogeneses is currently not clearly understood. These show dysregulated inflammatory responses, activation neurons, glial cells, neurovascular unit damage associated with excessive release proinflammatory cytokines, chemokines, neurotoxic mediators, infiltration peripheral immune cells into brain, as well entry mediators through damaged endothelial blood–brain barrier tight junction proteins. Activation leads to many molecules that cause neuroinflammation neurodegeneration. Gulf War Illness (GWI) myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) also dysfunctions. Currently, there no effective disease-modifying therapeutic options available for diseases. Human induced pluripotent stem cell (iPSC)-derived astrocytes, microglia, pericytes used models drug discovery. This review highlights certain recent trends in neuroinflammatory responses iPSC-derived applications

Language: Английский

Citations

29
The Promise and Potential of Brain Organoids DOI Creative Commons
Lena Smirnova, Thomas Härtung

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(21)

Published: Jan. 22, 2024

Brain organoids are 3D in vitro culture systems derived from human pluripotent stem cells that self-organize to model features of the (developing) brain. This review examines techniques behind organoid generation, their current and potential applications, future directions for field. possess complex architecture containing various neural cell types, synapses, myelination. They have been utilized toxicology testing, disease modeling, infection studies, personalized medicine, gene-environment interaction studies. An emerging concept termed Organoid Intelligence (OI) combines with artificial intelligence generate learning memory, goals modeling cognition enabling biological computing applications. allow neuroscience studies not previously achievable traditional techniques, transform drug development, understanding brain development disorders. The aspirational vision OI parallels origins intelligence, efforts underway map a roadmap toward its realization. In summary, constitute disruptive technology is rapidly advancing gaining traction across multiple disciplines.

Language: Английский

Citations

22
Potential Consciousness of Human Cerebral Organoids: on Similarity-Based Views in Precautionary Discourse DOI Creative Commons
Sarah Diner

Neuroethics, Journal Year: 2023, Volume and Issue: 16(3)

Published: Sept. 18, 2023

Abstract Advances in research on human cerebral organoids (HCOs) call for a critical review of current policies. A challenge the evaluation necessary regulations lies severe uncertainty about future trajectories currently very rudimentary stages neural cell cultures might take as technology progresses. To gain insights into organotypic cultures, ethicists, legal scholars, and neuroscientists rely resemblances to brain. They refer similarities structural or functional terms that have been established scientific practice validate models brain research. In ethical discourse, however, such are also used justify assumptions potential risk cause harm HCOs. Ethicists assume advances, will increasingly resemble brain, raising more complex issues. I argue reasoning is not justified given heterogeneity HCOs modified enable scientists pursue their goals. then discuss implications this line thought has advocates precautionary principle, focussing those suggestions which propose adopting presence bodily warning signs deemed worthy protection. doing so, illustrate prevalent similarity discourse ultimately disproportionately restricting conclude technology’s development, benefit from narrowing time frame anticipation.

Language: Английский

Citations

31
Limitations of human brain organoids to study neurodegenerative diseases: a manual to survive DOI Creative Commons

Nerea Urrestizala-Arenaza,

Sonia Cerchio,

Fabio Cavaliere

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: July 9, 2024

In 2013, M. Lancaster described the first protocol to obtain human brain organoids. These organoids, usually generated from human-induced pluripotent stem cells, can mimic three-dimensional structure of brain. While they recapitulate salient developmental stages brain, their use investigate onset and mechanisms neurodegenerative diseases still faces crucial limitations. this review, we aim highlight these limitations, which hinder organoids becoming reliable models study such as Alzheimer’s disease (AD), Parkinson’s (PD), amyotrophic lateral sclerosis (ALS). Specifically, will describe structural biological impediments, including lack an aging footprint, angiogenesis, myelination, inclusion functional immunocompetent microglia—all important factors in neurodegeneration AD, PD, ALS. Additionally, discuss technical limitations for monitoring microanatomy electrophysiology parallel, propose solutions overcome current thereby making a more tool model neurodegeneration.

Language: Английский

Citations

10
Midbrain organoids for Parkinson's disease (PD) - A powerful tool to understand the disease pathogenesis DOI

Harysh Winster Suresh Babu,

Sindduja Muthu Kumar,

Harsimrat Kaur

et al.

Life Sciences, Journal Year: 2024, Volume and Issue: 345, P. 122610 - 122610

Published: April 4, 2024

Language: Английский

Citations

9
Organoid, organ-on-a-chip and traditional Chinese medicine DOI Creative Commons
Jiayue Yang, Yu Jiang, Mingxing Li

et al.

Chinese Medicine, Journal Year: 2025, Volume and Issue: 20(1)

Published: Feb. 12, 2025

Abstract In the past few years, emergence of organoids and organ-on-a-chip (OOAC) technologies, which are complementary to animal models two-dimensional cell culture methods can better simulate internal environment human body, provides a new platform for traditional Chinese medicine (TCM) studies. Organoids OOAC techniques have been increasingly applied in fields drug screening, assessment development, personalized therapies, developmental biology, there some application cases TCM this review, we summarized current status using organoid technologies research provide key insights future study. It is believed that will play more important roles make greater contributions innovative development TCM.

Language: Английский

Citations

1
Lipid Rafts: The Maestros of Normal Brain Development DOI Creative Commons

Barbara Viljetić,

Senka Blažetić, Irena Labak

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(3), P. 362 - 362

Published: March 18, 2024

Lipid rafts, specialised microdomains within cell membranes, play a central role in orchestrating various aspects of neurodevelopment, ranging from neural differentiation to the formation functional neuronal networks. This review focuses on multifaceted involvement lipid rafts key neurodevelopmental processes, including differentiation, synaptogenesis and myelination. Through spatial organisation signalling components, facilitate precise events that determine fate during embryonic development adulthood. The evolutionary conservation underscores their fundamental importance for structural complexity nervous system all species. Furthermore, there is increasing evidence environmental factors can modulate composition function influence processes. Understanding intricate interplay between neurodevelopment not only sheds light mechanisms governing brain but also has implications therapeutic strategies aimed at cultivating networks addressing disorders.

Language: Английский

Citations

7
Biofabrication of Neural Organoids: An Experiential Learning Approach for Instructional Laboratories DOI
Caroline Cvetkovic, Sarah Lindley, Holly Golecki

et al.

Biomedical Engineering Education, Journal Year: 2024, Volume and Issue: 4(2), P. 409 - 419

Published: April 30, 2024

Language: Английский

Citations

4
Transgenerational hormesis in healthy aging and antiaging medicine from bench to clinics: Role of food components DOI
Vittorio Calabrese, Naomi Osakabe, Rosalba Siracusa

et al.

Mechanisms of Ageing and Development, Journal Year: 2024, Volume and Issue: 220, P. 111960 - 111960

Published: July 5, 2024

Language: Английский

Citations

4
The Neuroethical Nexus of Brain Organoids and AI DOI
Suyesha Singh, Paridhi Jain

Advances in computational intelligence and robotics book series, Journal Year: 2025, Volume and Issue: unknown, P. 161 - 178

Published: March 21, 2025

The advancement of brain organoids has revolutionized the fields biotechnology and neuroscience. Brain are 3-D structures human developed from human's pluripotent cells, which mimic functionality brain. Organoid intelligence cumulates artificial intelligence, making it possible to develop a biohybrid system with heightened cognitive capacities. This paper aims critically analyze concerns surrounding organoid highlight scientific progress ethical considerations, ensuring that new technologies contribute community while maintaining fundamental moral principles. findings current work will be useful for decision makers, neuroscientists, ethicists, public promote environmentally conscious innovation practice in an way. serves as call action addressing ongoing challenges, advancing field, utilizing effectively context.

Language: Английский

Citations

0