Effects of macrophages in OSCC progression

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 14, 2025

Macrophages are crucial immune cells within the tumor microenvironment (TME), involved in regulating proliferation, invasion, metastasis, ECM remodeling, angiogenesis, and immunosuppression. Although more experimental evidence clinical data indicate that macrophages onset progression of oral squamous cell carcinoma (OSCC), exact pathogenesis OSCC associated with has not been fully elucidated. Enhanced knowledge molecular mechanisms involving will aid creation treatments targeted specifically at macrophages. This review outlines pro-tumoral anti-tumoral effects OSCC, emphasizing interaction between It can provide theoretical basis for establishment complex regulatory network centered on explore novel therapeutic strategies OSCC.

Language: Английский

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Baicalin induces ferroptosis in oral squamous cell carcinoma by suppressing the activity of FTH1

The Journal of Gene Medicine, Journal Year: 2024, Volume and Issue: 26(2)

Published: Feb. 1, 2024

Abstract Background This study investigated the role of ferroptosis‐related gene FTH1 in oral squamous cell carcinoma (OSCC) and evaluated therapeutic potential baicalin OSCC treatment. Methods A prognostic model was established by bioinformatic analysis, consisting 12 ferroptosis related genes (FRGs), selected as most significantly up‐regulated FRGs. The clinical correlation samples both immunohistochemical characterizations. effects on migration, invasion, epithelial–mesenchymal transition (EMT) proliferation were determined wound healing assays, transwell western blotting 5′‐ethynl 2′‐deoxyuridine respectively. tested via markers Mito Tracker staining. In addition, cells confirmed using EMT, assays. Results FRGs predictive prognosis for patients, expression identified samples, which highly associated with survival, immune infiltration drug sensitivity. Moreover, knocking down inhibited proliferation, EMT invasive phenotypes, but induced (Cal27 SCC25). Furthermore, directly suppressed cells, effectively promoted well targeting FTH1. Conclusions has demonstrated that is a target treatment, provided evidence offers promising alternative

Language: Английский

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Harnessing tetrahedral framework nucleic acids for enhanced delivery of microRNA‐149‐3p: A new frontier in oral squamous cell carcinoma therapy

Cell Proliferation, Journal Year: 2024, Volume and Issue: 57(8)

Published: April 26, 2024

Abstract Oral squamous cell carcinoma (OSCC), a type of malignant tumour that primarily occurs in the oral mucosa, has drawn considerable attention owing to its aggressive growth and potentially high metastatic rate. Surgical resection is primary treatment method for OSCC typically combined with radiation therapy chemotherapy. microRNA‐149‐3p (miR‐149) negative regulator Pi3k/Akt pathway can effectively inhibit proliferation cells. However, application miR‐149 limited relatively low efficiency cellular uptake poor stability when used alone. To overcome these challenges, this study adopted novel nucleic acid nanostructured material, tetrahedral framework acids (tFNAs). The use tFNAs as carriers assemble T‐miR‐149 complex reduced expression Pi3k Akt involved tumorigenesis alterations proteins related apoptosis. results indicated bionic drug delivery system an effective suppressive effect on mice, revealing potential clinical value OSCC.

Language: Английский

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Extracellular Matrix Ligands Modulate the Endothelial Progenitor Cell Secretome for Enhanced Angiogenesis

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Lycorine hydrochloride Suppresses the Proliferation and Invasion of Esophageal Cancer by Targeting TRIM22 and Inhibiting the JAK2/STAT3 and Erk Pathways

Cancers, Journal Year: 2025, Volume and Issue: 17(5), P. 718 - 718

Published: Feb. 20, 2025

Tumor metastasis and poor drug efficacy are two of the most common causes therapeutic failure in cancer patients. The underlying molecular mechanism requires further exploration, novel effective curative strategies urgently needed. Nature is a rich source drugs, Lycorine hydrochloride (Lyc.HCL) natural alkaloid with tremendous potential. However, mechanisms its antitumor activity still unknown. In current study, we investigated effects Lyc.HCL against esophageal squamous cell carcinomas (ESCCs), which pose serious threats to human life. An MTS assay clone formation were used assess viability ESCC lines after treatment vitro. Apoptosis cycle regulation analyzed using flow cytometry. Wound healing Transwell assays analyze migration, while invasion was Matrigel assay. We detected expression tripartite motif-containing 22 (TRIM22) through immunohistochemistry Western blotting. A docking experiment performed explore targets Lyc.HCL. levels Janus kinase 2 (JAK2)/signal transducer activator transcription 3 (STAT3) phosphoinositide 3-kinase (PI3K)/protein B (AKT)/extracellular signal-regulated (Erk) pathway components rescue determine potential role TRIM22. addition, explored vivo anti-ESCC by it treat tumor-bearing mice. found inhibit carcinoma proliferation both vitro blocking at G2 phase, inhibiting migration invasion. that TRIM22 protein highly expressed ESCCs but not normal tissue. directly targeted TRIM22, decreasing JAK2/STAT3 Erk signaling pathways, vivo. Using animal experiments, observed depletion delayed tumor growth, this effect significantly reversed upon overexpression. Taken together, these findings demonstrate can effectively suppress targeting regulating pathways. These results suggest may serve as for ESCC, emerging promising target treatment.

Language: Английский

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TIMP4 serves as a novel potential prognostic biomarker for oral squamous cell carcinoma

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 21, 2025

Language: Английский

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Identifying pathological myopia associated genes with GenePlexus in protein-protein interaction network

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: March 5, 2025

Pathological myopia, a severe form of is characterized by an extreme elongation the eyeball, leading to various vision-threatening complications. It broadly classified into two primary types: high which primarily involves excessive axial length eye with potential for reversible vision loss, and degenerative associated progressive irreversible retinal damage. Leveraging data from DisGeNET, reporting 184 genes linked myopia 39 we employed GenePlexus methodology in conjunction screening tests further explore genetic landscape pathological myopia. Our comprehensive analysis resulted discovery 21 new 133 significant confidence. Among these findings, such as ADCY4, regulator cAMP pathway, were functionally while THBS1, involved collagen degradation, was closely pathophysiology These previously unreported play crucial roles underlying mechanisms thereby emphasizing complexity multifactorial nature this condition. The importance our study resides uncovering associations provision biomarkers early screening, identification therapeutic targets.

Language: Английский

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Engineering a dynamic extracellular matrix using thrombospondin-1 to propel hepatocyte organoids reprogramming and improve mouse liver regeneration post-transplantation

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101700 - 101700

Published: March 1, 2025

Hepatocyte organoids (HOs) hold significant potential for constructing bioartificial liver construction, toxicology research, and failure therapies. However, challenges such as difficulties in induced pluripotent stem cells (iPSCs) harvest differentiation, safety concerns of tumor-derived matrices, limited primary cell regulation hinder clinical applications. In this study, we developed a non-tumor-derived decellularized extracellular matrix (dECM) system with tunable mechanical properties viscoelasticity to enhance proliferation organoid functionality using thrombospondin-1 (THBS1). Nanoindentation transcriptomic analysis revealed that THBS1 mediates adaptation remodeling between ECM proteins, exhibiting native tissue-like up-regulated reprogramming transcriptional factors KLF4 SOX2 via the YAP/TAZ pathway. Transplanting HOs presenting effects into 70 % hepatectomy model demonstrated improved regeneration, underscoring THBS1-based dynamic manipulation regeneration.

Language: Английский

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LncRNA HOTAIR Interaction With WTAP Promotes m⁶A Methyltransferase Complex Assembly and Posterior Capsule Opacification Formation by Increasing THBS1

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(5), P. 20 - 20

Published: May 9, 2025

To explore the role of long non-coding RNAs (lncRNAs) and N6-methyladenosine (m6A) in posterior capsule opacification (PCO) their underlying mechanisms. The localization lncRNAs proteins was analyzed using fluorescence situ hybridization immunofluorescence staining. RNA m6A quantification, immunoprecipitation, co-immunoprecipitation, MeRIP-seq, MeRIP-qPCR, western blotting, wound healing, Transwell assays were applied to elucidate levels lncRNA HOX transcript antisense intergenic (HOTAIR) methylation increased significantly during epithelial-mesenchymal transition (EMT) lens epithelial cells (LECs). HOTAIR promoted EMT methyltransferase activity but had no effect on not modified by m6A. Nevertheless, interacted with WT1-associated protein (WTAP), a key writer protein, facilitating WTAP-mediated recruitment METTL3-METTL14 heterodimers enhancing modification. HOTAIR/WTAP complex elevated levels, thrombospondin 1 (THBS1) expression, LECs. LncRNA enhances assembly WTAP/METTL3/METTL14 promotes LECs upregulating modification THBS1 expression. Targeting HOTAIR/WTAP/THBS1 pathway may prevent or treat PCO.

Language: Английский

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Forsythiaside A suppresses renal fibrosis and partial epithelial-mesenchymal transition by targeting THBS1 through the PI3K/AKT signaling pathway

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 129, P. 111650 - 111650

Published: Feb. 10, 2024

Renal fibrosis is a key feature of chronic kidney disease (CKD) progression, whereas no proven effective anti-fibrotic treatments. Forsythiaside A (FTA), derived from Forsythia suspense, has been found to possess nephroprotective properties. However, there limited research on its effects, and mechanism action remains unknown. This study aimed investigate the suppressive effects FTA renal explore underlying mechanisms. In vitro, we established HK2 cell model induced by transforming growth factor β1 (TGF-β1), in vivo, used mice unilateral ureteral obstruction (UUO). CCK-8 assay, qRT-PCR, Western blotting, immunofluorescence, flow cytometry, histological staining, immunohistochemistry, TUNEL RNA transcriptome sequencing, molecular docking were performed. The results showed that (40 μM or 80 μM) treatment improved viability suppressed TGF-β1-induced fibrotic changes partial epithelial-mesenchymal transition (EMT). Furthermore, reversed activation PI3K/AKT signaling pathway, THBS1 was identified as target gene. We knockdown pathway reduced EMT-related protein level. Conversely, overexpression activated exacerbated EMT. administered (30 60 mg/kg) for 2 weeks, demonstrated administration significantly mitigated tubular injury, tubulointerstitial fibrosis, EMT, apoptosis. conclusion, inhibited EMT targeting inhibiting pathway.

Language: Английский

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