Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 174, P. 104189 - 104189
Published: Sept. 27, 2024
Language: Английский
Physiological and Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 131, P. 102274 - 102274
Published: March 25, 2024
Language: Английский
Citations
7
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3271 - 3271
Published: March 13, 2024
The advent of CRISPR/Cas9 technology has revolutionized genome editing, enabling the attainment once-unimaginable goals. CRISPR/Cas's groundbreaking attributes lie in its simplicity, versatility, universality, and independence from customized DNA-protein systems, erasing need for specialized expertise broadening scope applications. It is therefore more used modification including generation mutants. Beyond such editing scopes, recent development novel or modified Cas-based systems spawned an array additional biotechnological tools, empowering both fundamental applied research. Precisely targeting DNA RNA sequences, CRISPR/Cas system been harnessed fields as diverse gene regulation, deepening insights into expression, epigenetic changes, spatial organization, chromatin dynamics. Furthermore, it aids imaging sequencing, well effective identification countering viral pathogens plants animals. All all, non-editing aspect exhibits tremendous potential across domains, diagnostics, biotechnology, This article reviews critically evaluates primary CRISPR/Cas-based tools developed animals, underlining their transformative impact.
Language: Английский
Citations
5
Foods, Journal Year: 2025, Volume and Issue: 14(2), P. 286 - 286
Published: Jan. 16, 2025
Tomato, as the vegetable queen, is cultivated worldwide due to its rich nutrient content and unique flavor. Nondestructive technology provides efficient noninvasive solutions for quality assessment of tomatoes. However, processing substantial datasets achieve a robust model enhance detection performance nondestructive great challenge until deep learning developed. The aim this paper provide systematical overview principles application three categories techniques based on mechanical characterization, electromagnetic well electrochemical sensors. Tomato analyzed, characteristics different are compared. Various data analysis methods explored applications in tomato using with also summarized. Limitations future expectations industry by along discussed. ongoing advancements optical equipment lead promising outlook agricultural engineering.
Language: Английский
Citations
0
Plant Biotechnology Reports, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 29, 2025
Language: Английский
Citations
0
Published: April 28, 2024
Viral diseases pose a significant threat to tomato crops (Solanum lycopersicum L.), one of the world's most economically important vegetable crops. The limited genetic diversity cultivated tomatoes contributes their high susceptibility viral infections. To address this challenge, breeding programs must harness resources found in native populations and wild relatives. Breeding efforts may aim develop broad-spectrum resistance against virome. identify viruses naturally infecting 19 advanced lines, derived from tomatoes, high-throughput sequencing (HTS) small RNAs validation by PCR RT-PCR were used. Single mixed infections with Tomato mosaic virus (ToMV), golden (ToGMoV), Pepper huasteco yellow vein (PHYVV) detected. complete consensus genomes three variants Mexican ToMV isolates reconstructed, potentially forming new clade distinct 3' UTR. absence reported mutations associated resistance-breaking suggests that Tm-1, Tm-2, Tm-22 genes could theoretically be used confer resistance. However, mutation rates 63 nucleotide insertion UTR suggest need evaluate known these variants. precise identification characterization infect germplasm as strategy for selecting appropriate disease management guiding efforts.
Language: Английский
Citations
2
Food Bioscience, Journal Year: 2024, Volume and Issue: 59, P. 104241 - 104241
Published: April 30, 2024
Language: Английский
Citations
1
Viruses, Journal Year: 2024, Volume and Issue: 16(6), P. 888 - 888
Published: May 31, 2024
Viral diseases pose a significant threat to tomato crops (Solanum lycopersicum L.), one of the world’s most economically important vegetable crops. The limited genetic diversity cultivated tomatoes contributes their high susceptibility viral infections. To address this challenge, breeding programs must harness resources found in native populations and wild relatives. Breeding efforts may aim develop broad-spectrum resistance against virome. identify viruses naturally infecting 19 advanced lines, derived from tomatoes, high-throughput sequencing (HTS) small RNAs confirmation with PCR RT-PCR were used. Single mixed infections mosaic virus (ToMV), golden (ToGMoV), pepper huasteco yellow vein (PHYVV) detected. complete consensus genomes three variants Mexican ToMV isolates reconstructed, potentially forming new clade distinct 3’ UTR. absence reported mutations associated resistance-breaking suggests that Tm-1, Tm-2, Tm-22 genes could theoretically be used confer resistance. However, mutation rates 63 nucleotide insertion UTR, as well amino acid ORFs encoding 126 KDa, 183 MP isolates, suggest it is necessary evaluate capacity these overcome genes. This evaluation, along characterization lines using molecular markers linked resistant genes, will addressed future studies part strategy. study emphasizes importance HTS for accurate identification plant infect germplasm based on genome sequences. provides crucial insights select appropriate disease management strategies guide toward development virus-resistant varieties.
Language: Английский
Citations
1
Agriculture, Journal Year: 2024, Volume and Issue: 14(7), P. 1192 - 1192
Published: July 19, 2024
Tomato (Solanum lycopersicum L.) is a crop that affected by more than hundred viral species. De la pera local varietal type of tomato very popular in southeastern Spain. However, it highly susceptible to several viruses, such as yellow leaf curl virus (TYLCV), which considered one the most important diseases crops and limiting factor for production both outdoor protected crops, making difficult eradicate. This study shows effect gene introgression on performance traditional lines combining two genes offer tolerance TYLCV, Ty-1 ty-5, some yield quality traits antioxidant capacity fruits. Two pear breeding families, UMH175 UMH220, were evaluated. Four from each families with all homozygous combinations ty-5 studied. The results showed allele produced slight negative yield, mean fruit weight, total soluble solids, titratable acidity, contrast Ty-1, large effect. None introgressions compounds. promising use programs.
Language: Английский
Citations
1
Plants, Journal Year: 2024, Volume and Issue: 13(16), P. 2269 - 2269
Published: Aug. 15, 2024
Tomato (Solanum lycopersicon L.) is one of the most widely consumed and produced vegetable crops worldwide. It offers numerous health benefits due to its rich content many therapeutic elements such as vitamins, carotenoids, phenolic compounds. Biotic stressors bacteria, viruses, fungi, nematodes, insects cause severe yield losses well decreasing fruit quality. Conventional breeding strategies have succeeded in developing resistant genotypes, but these approaches require significant time effort. The advent state-of-the-art genome editing technologies, particularly CRISPR/Cas9, provides a rapid straightforward method for high-quality biotic stress-resistant tomato lines. advantage over other ability make precise, minute adjustments without leaving foreign DNA inside transformed plant. has been precisely modified via CRISPR/Cas9 induce resistance genes or knock out susceptibility genes, resulting lines common bacterial, fungal, viral diseases. This review recent advances application with stress.
Language: Английский
Citations
1
Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 338, P. 113687 - 113687
Published: Sept. 29, 2024
Language: Английский
Citations
1