Diselenide bond-stapled β-hairpin peptide-loaded hydrogel system for boosting oncolytic immunotherapy DOI

Shengxin Lu,

Xiao-kun Zhang, Jiayi Lin

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 159076 - 159076

Published: Dec. 1, 2024

Language: Английский

Engineering a genomically recoded organism with one stop codon DOI Creative Commons

Michael W. Grome,

Michael Nguyen,

Daniel W. Moonan

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

The genetic code is conserved across all domains of life, yet exceptions have revealed variations in codon assignments and associated translation factors1-3. Inspired by this natural malleability, synthetic approaches demonstrated whole-genome replacement synonymous codons to construct genomically recoded organisms (GROs)4,5 with alternative codes. However, no efforts fully leveraged factor plasticity degeneracy compress function a single assess the possibility non-degenerate code. Here we describe construction characterization Ochre, GRO that compresses translational into codon. We replaced 1,195 TGA stop TAA ∆TAG Escherichia coli C321.∆A4. then engineered release 2 (RF2) tRNATrp mitigate native UGA recognition, translationally isolating four for functions. Ochre thus utilizes UAA as sole codon, UGG encoding tryptophan UAG reassigned multi-site incorporation two distinct non-standard amino acids proteins more than 99% accuracy. degenerate represents an important step toward 64-codon will enable precise production multi-functional unnatural encoded chemistries broad utility biotechnology biotherapeutics.

Language: Английский

Citations

3
tRNA engineering strategies for genetic code expansion DOI Creative Commons

YouJin Kim,

Suho Cho,

Joo‐Chan Kim

et al.

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: March 7, 2024

The advancement of genetic code expansion (GCE) technology is attributed to the establishment specific aminoacyl-tRNA synthetase/tRNA pairs. While earlier improvements mainly focused on synthetases, recent studies have highlighted importance optimizing tRNA sequences enhance both unnatural amino acid incorporation efficiency and orthogonality. Given crucial role tRNAs in translation process their substantial impact overall GCE efficiency, ongoing efforts are dedicated development engineering techniques. This review explores diverse approaches provides illustrative examples context GCE, offering insights into user-friendly implementation technology.

Language: Английский

Citations

12
Understanding Selenoproteins: Structural insights, biological functions and transformative applications in therapeutics DOI

Bhairav Prasad,

Akanksha Akanksha,

Palki Sahib Kaur

et al.

Process Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Facile Generation of Cyanoselenocysteine as a Vibrational Label for Measuring Protein Dynamics on Longer Time Scales by 2D IR Spectroscopy DOI
Noor Ali, Swapnil Singh, Chaitrali Sengupta

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Two-dimensional infrared (2D IR) spectroscopy is a powerful technique for measuring molecular heterogeneity and dynamics with high spatiotemporal resolution. The methods can be applied to characterize specific residues of proteins by incorporating frequency-resolved vibrational labels. However, the time scale that 2D IR measure limited label's excited-state lifetime due decay absorption bands. To extend this scale, labels longer lifetimes are sought. An effective approach inhibiting intramolecular energy relaxation isolate vibration from rest molecule inserting heavy atom bridge. Although strategy has been demonstrated through generation functionalized amino acids, straightforward route their selective incorporation into often unclear. A facile attachment cyano group at cysteine generate thiocyanate contributed its adoption as label proteins. We demonstrate an analogous used introducing cyanoselenocysteine selenocyanate containing heavier bridge atom. confirm pump–probe 100–250 ps, depending on solvent, which enable collection spectra frequency scales.

Language: Английский

Citations

0
Tuning tRNAs for improved translation DOI Creative Commons

Joshua L. Weiss,

J. C. Decker,

Ariadna Bolano

et al.

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: June 25, 2024

Transfer RNAs have been extensively explored as the molecules that translate genetic code into proteins. At this interface of genetics and biochemistry, tRNAs direct efficiency every major step translation by interacting with a multitude binding partners. However, due to variability tRNA sequences abundance diverse post-transcriptional modifications, guidebook linking specific translational outcomes has yet be elucidated. Here, we review substantial efforts collectively uncovered engineering principles can used guide for tuning fidelity. These allowed development basic research, expansion non-canonical amino acids, therapeutics.

Language: Английский

Citations

3
Selenoprotein K at the intersection of cellular pathways DOI

Atinuke Odunsi,

Mariia A. Kapitonova,

G Woodward

et al.

Archives of Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: unknown, P. 110221 - 110221

Published: Nov. 1, 2024

Language: Английский

Citations

0
Diselenide bond-stapled β-hairpin peptide-loaded hydrogel system for boosting oncolytic immunotherapy DOI

Shengxin Lu,

Xiao-kun Zhang, Jiayi Lin

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 159076 - 159076

Published: Dec. 1, 2024

Language: Английский

Citations

0