Detection of Candidate Genes and Development of KASP Markers for Pod Length and Pod Width by Combining Genome-Wide Association and Transcriptome Sequencing in Vegetable Soybean DOI Creative Commons
Dongqing Dai, Lu Huang, Xiaoyan Zhang

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(11), P. 2654 - 2654

Published: Nov. 11, 2024

Vegetable soybeans are one of the most important vegetable types in East Asia. The yield is considerably influenced by size their pods. To facilitate understanding genetic basis pod length and width soybeans, we conducted a genome-wide association study (GWAS) transcriptome sequencing. Four quantitative trait loci, namely, qGPoL1, qGPoL2, qGPoW1, qGPoW2, were mapped via GWAS analysis. Through integration gene function annotation, sequencing, expression pattern analysis, identified Glyma.06G255000 Glyma.13G007000 as key determinants respectively. Furthermore, two kompetitive allele-specific polymerase chain reaction (KASP) markers, S06-42138365 (A/T) S13_628331 (A/T), developed effectively validated 27 soybean accessions. Overall, our research genes that regulate determined KASP markers for molecular marker-assisted selection breeding. These findings have crucial implications improvement crops can contribute to development efficient breeding strategies.

Language: Английский

Conjunctive BSA-Seq and BSR-Seq to Map the Genes of Yellow Leaf Mutations in Hot Peppers (Capsicum annuum L.) DOI Open Access

Guosheng Sun,

Changwei Zhang,

Xi Shan

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(9), P. 1115 - 1115

Published: Aug. 23, 2024

Yellow leaf mutations have been widely used to study the chloroplast structures, pigment synthesis, photosynthesis mechanisms and chlorophyll biosynthesis pathways across various species. For this study, a spontaneous mutant with yellow color named 96-140YBM was employed explore primary genetic elements that lead variations in of hot peppers. To identify genes associated phenotypes, we applied sequencing-based Bulked Segregant Analysis (BSA-Seq) combined BSR-Seq. We identified 4167 differentially expressed (DEGs) pool compared wild-type pool. The results indicated DEGs were involved zeatin biosynthesis, plant hormone signal transduction, transduction mechanisms, post-translational modification protein turnover. A total 437 candidates by BSA-Seq, while BSR-Seq pinpointed four candidate regions chromosomes 8 9, containing 222 genes. Additionally, combination BSA-Seq showed there 113 overlapping between two methods, among which common previously reported be related development chloroplasts, photomorphogenesis formation chloroplasts biogenesis. qRT-PCR analysis higher expression levels Among candidates, DEG

Language: Английский

Citations

2
Identification and functional analysis of GmPasL regulating pod color in vegetable soybean DOI Creative Commons
Dongqing Dai, Lu Huang, Xiaoyan Zhang

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 4, 2024

Language: Английский

Citations

1
Genetic Mapping and Characterization of the Clubroot Resistance Gene BraPb8.3 in Brassica rapa DOI Open Access
Liyan Kong, Yi Yang,

Yufei Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10462 - 10462

Published: Sept. 28, 2024

Clubroot, a significant soil-borne disease, severely impacts the productivity of cruciferous crops. The identification and development clubroot resistance (CR) genes are crucial for mitigating this disease. This study investigated genetic inheritance within an F2 progeny derived from cross resistant parent, designated “377”, susceptible “12A”. Notably, “377” exhibited robust to “KEL-23” strain Plasmodiophora brassicae, causative agent clubroot. Genetic analyses suggested that observed is controlled by single dominant gene. Through Bulked Segregant Analysis sequencing (BSA-seq) preliminary gene mapping, we localized CR locus, as BraPb8.3, 1.30 Mb genomic segment on chromosome A08, flanked markers “333” “sau332-1”. Further fine mapping precisely narrowed down position BraPb8.3 173.8 kb region between “srt8-65” “srt8-25”, where identified 22 genes, including Bra020861 with TIR-NBS-LRR domain Bra020876 LRR domain. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) confirmed both exhibit increased expression levels in parent following inoculation P. thereby underscoring their potential key implicated BraPb8.3-mediated resistance. not only identifies molecular associated but also enriches resources available breeding programs aimed at enhancing

Language: Английский

Citations

0
Detection of Candidate Genes and Development of KASP Markers for Pod Length and Pod Width by Combining Genome-Wide Association and Transcriptome Sequencing in Vegetable Soybean DOI Creative Commons
Dongqing Dai, Lu Huang, Xiaoyan Zhang

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(11), P. 2654 - 2654

Published: Nov. 11, 2024

Vegetable soybeans are one of the most important vegetable types in East Asia. The yield is considerably influenced by size their pods. To facilitate understanding genetic basis pod length and width soybeans, we conducted a genome-wide association study (GWAS) transcriptome sequencing. Four quantitative trait loci, namely, qGPoL1, qGPoL2, qGPoW1, qGPoW2, were mapped via GWAS analysis. Through integration gene function annotation, sequencing, expression pattern analysis, identified Glyma.06G255000 Glyma.13G007000 as key determinants respectively. Furthermore, two kompetitive allele-specific polymerase chain reaction (KASP) markers, S06-42138365 (A/T) S13_628331 (A/T), developed effectively validated 27 soybean accessions. Overall, our research genes that regulate determined KASP markers for molecular marker-assisted selection breeding. These findings have crucial implications improvement crops can contribute to development efficient breeding strategies.

Language: Английский

Citations

0