Bioinformatics analysis of genes associated with disulfidptosis in spinal cord injury

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0318016 - e0318016

Published: Feb. 14, 2025

Research findings indicate that programmed cell death (PCD) plays a pivotal role in the pathophysiology of spinal cord injury (SCI), and recently discovered form death, disulfidptosis, has emerged as novel phenomenon. However, characterization disulfidptosis-related genes SCI remains insufficiently explored. We retrieved SCI-related data from Gene Expression Omnibus (GEO) database identified three key associated with disulfidptosis human (CAPZB, SLC3A2, TLN1), whose mediated signaling pathways are closely intertwined SCI. Subsequent functional enrichment analysis suggested these may regulate multiple exert corresponding roles pathology. Moreover, we predicted potential targeted drugs for along their transcription factors constructed an intricate regulatory network. CIBERSORT revealed CAPZB, TLN1 might be implicated modulating changes within immune microenvironment individuals Our study provides compelling evidence confirming significant involvement following while offering valuable insights into its underlying pathological mechanisms.

Language: Английский

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Proteomics and Expression of HIF2α/BNIP3L Signaling in Yak Brains at Different Altitudes

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1675 - 1675

Published: Feb. 16, 2025

The yak, a unique inhabitant of low-oxygen environments, exhibits brain adaptability to hypoxic conditions. However, the impact hypoxia on yak proteomics and expression HIF2α/BNIP3L signaling pathway remains unexplored. This study utilized TMT-based analysis identify differentially expressed proteins (DEPs) in cerebral cortexes 9-month-old yaks at high (n = 3) low altitudes. Additionally, qRT-PCR, Western blot, immunohistochemistry, immunofluorescence were used analyze HIF2α, BNIP3L, Beclin1, LC3-II, cleaved caspase-3 various regions from both altitude groups. KEGG revealed that DEPs mainly concentrated synthesis metabolism, DNA replication, repair pathways. Specifically, autophagy attracted our attention due its absence other animals. LC3-II increased significantly. Furthermore, results qRT-PCR blot showed that, same altitude, mRNA protein levels Beclin1 hippocampi significantly higher than those thalami, medulla oblongatae, cerebella (p < 0.05), while was not different among > 0.05). within region, high-altitude low-altitude yaks. Moreover, there no difference between Immunohistochemistry HIF2α-positive nucleus cytoplasm neurons, cytoplasm. caspase-3, NeuN co-located neurons cortex, hippocampus, thalamus, oblongata, cerebellum, respectively. offers complete characterization cortex proteome indicates these are more resistant hypoxia. In addition, may enhance tissue adaptation

Language: Английский

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Decoding Quantitative Traits in Yaks: Genomic Insights for Improved Breeding Strategies

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(5), P. 350 - 350

Published: May 12, 2025

The yak (Bos grunniens), the only large domesticated species endemic to Qinghai–Tibet Plateau, is a vital resource for local livelihoods and regional economic sustainability. However, breeding faces significant challenges, including limited understanding of genetic architecture underlying quantitative traits, inadequate advanced strategies, sterility hybrid offspring from yak–cattle crosses. These constraints have hindered progress in key production traits. To address these issues, integrating modern genomic tools into programs imperative. This review explores application potential molecular marker-assisted selection (MAS) prediction (GP) improvement. We systematically evaluate critical components pipelines, including: (1) phenotypic trait assessment, (2) sample collection (3) reference population design, (4) high-throughput genotyping (via genome sequencing SNP arrays), (5) predictive model development, (6) heritability estimation. By synthesizing current advances methodologies, this work aims provide framework leveraging technologies enhance efficiency, preserve diversity, accelerate gains populations.

Language: Английский

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Localized delivery of Roxadustat via cubosomes-based thermosensitive in-situ hydrogel enhances diabetic wound healing by stabilizing HIF-1α in rats

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 100, P. 106127 - 106127

Published: Aug. 29, 2024

Language: Английский

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Genetic structure analysis of yak breeds and their response to adaptive evolution

Genomics, Journal Year: 2024, Volume and Issue: 116(5), P. 110933 - 110933

Published: Aug. 30, 2024

Yaks are crucial genetic resources in the Tibetan Plateau and surrounding regions. Throughout long process of domestication, natural artificial selection pressures have enabled yaks to demonstrate adaptive characteristics environment terms physiological structure molecules, but no systematic cell analysis has been carried out on this phenomenon yaks. Here, population diversity yak were studied by WGRS, genes related adaptability excavated. Combined with scRNA-seq method, transcription map lung tissue skin was constructed, which provided a new comprehensive insight into adaptability. The showed that there obvious differentiation between TZ _ other seven populations, while significant exchange PL SB at high altitude. WGRS revealed gene HIF1A altitude adaptation expressed various types, EPAS1 predominantly epithelial endothelial cells tissue. Endothelial play critical role hypoxia-adapted VEGF signaling, correlates closely expression KDR VEGFA monocytes. Furthermore, signal High vs Low yak, 19.8 % overlapped screened scRNA-seq, including coat color such as RORA, BNC2, KIT. Notably, BNC2 is associated melanin deposition shows levels HS cells. Additionally, GRN melanocytes SORT1 IRS an important communication IRS. These findings offer insights polymorphism provide valuable reference for future research high-altitude mammals, potentially even human genetics.

Language: Английский

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Development and characterization of an immortalized nasopharyngeal epithelial cell line to explore airway physiology and pathology in yak (Bos grunniens)

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: July 17, 2024

Airway epithelial cells play a crucial role in investigating the physiological and pathological mechanisms of respiratory tract yaks, species whose unique system has garnered extensive interest. Despite this growing interest, there currently are no available airway cell lines from underscoring need to establish yak line. Therefore, our objective was isolate population primary nasopharyngeal (pYNE) transform them into immortalized (iYNE), assessing their suitability as an

Language: Английский

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PMMA-induced biofilm promotes Schwann cells migration and proliferation mediated by EGF/Tnc/FN1 to improve sciatic nerve defect

Heliyon, Journal Year: 2024, Volume and Issue: 10(17), P. e37231 - e37231

Published: Sept. 1, 2024

The purpose of this study is to investigate the role PMMA-induced biofilm in nerve regeneration compared with silicone-induced involved mechanism.

Language: Английский

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Silk fibroin/gelatin electrospun nanofibrous dressing loaded with Roxadustat accelerates wound healing in diabetic rats via HIF-1α stabilization

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 106439 - 106439

Published: Nov. 1, 2024

Language: Английский

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